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61.
62.
This study describes an ultrathin-layer sodium dodecyl sulfate (SDS) disc electrophoresis in polyacrylamide gels of a thickness of only 150 microm. By use of 2-amino-2-methyl-1,3-propanediol/glycine instead of traditional Tris/HCl buffer in the resolving phase of the gel, proteins with a wide range of molecular sizes (10 kDa to over 220 kDa) are separated in unusually low-concentrated gels (4%T, 3.3%C). 2-Amino-2-methyl-1,3-propanediol in the resolving part of the gel contributes to stabilization of the pH value at 8.8, while glycine improves destacking as well as separation of small proteins from the bulk of stacked SDS. This method combines both the advantages of conventional slab-gel electrophoresis and capillary gel electrophoresis. It is easy to apply and well suited for all further miniaturization attempts. 相似文献
63.
The synthesis of C8-glycomimetics is described from C2-symmetrical polyhydroxylated cyclooctenes derived from carbocyclisation of enantiomerically pure 1,9-dienes by ring closing metathesis. Their obtention notably involved either hydroboration followed by oxidation to carbasugars or to cyclooctanones then reductive amination, or formation of a cis-cyclic sulfate followed by successive introduction of an azido group, reduction and subsequent reductive amination. The biological activity of the C8-carbasugars and related aminocyclitols, analogous to voglibose, has been evaluated towards several commercially available glycosidases. 相似文献
64.
The mechanism of photooxidation of phenothiazine in liquid and frozen (77 K) aqueous micellar solutions of the surfactant (sodium dodecyl sulfate) was studied by ESR. The main reaction of electrons formed by the photochemical oxidation of phenothiazine in a liquid micellar solution of the anionic surfactant is the reduction of molecular oxygen dissolved in the aqueous bulk phase. 10-Methylphenothiazine was used as a stable radical cation probe in a liquid solution. The influence of electrolytes on the photoionization yield in both frozen and liquid solutions of the surfactant was studied. 相似文献
65.
Yohannes G Wiedmer SK Tuominen EK Kinnunen PK Riekkola ML 《Analytical and bioanalytical chemistry》2004,380(5-6):757-766
Lipid membranes are well recognized ligands that bind peripheral and integral proteins in a specific manner and regulate their function. Cytochrome c (cyt c) is one of the partner peripheral protein that binds to the lipid membranes via electrostatic and hydrophobic interactions. In this study, asymmetrical flow field-flow fractionation (AsFlFFF) was used to compare the interactions of cyt c with the acidic phospholipid 1,2-dimyristoyl-sn-glycero-3-phospho-rac-glycerol (DMPG), oleic acid (OA), and sodium dodecyl sulfate (SDS). The influence of pH and the cyt c–lipid molar mass ratios were evaluated by monitoring the diffusion coefficients and particle diameter distributions obtained for the free and lipid-bound protein. The hydrodynamic particle diameter of cyt c (pI 10) was 4.1 nm at pH 11.4 and around 4.2 nm at pH 7.0 and 8.0. Standard molar mass marker proteins were used for calibration to obtain the molar masses of free cyt c and its complexes with lipids. AsFlFFF revealed the binding of cyt c to DMPG and to OA to be mainly electrostatic. In the absence of electrostatic interactions, minor complex formation occurred, possibly due to the extended lipid anchorage involving the hydrophobic cavity of cyt c and the hydrocarbon chains of DMPG or SDS. The possibility of the formation of the molten globule state of cyt c, induced by the interaction between cyt c and lipids, is discussed.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
66.
Dynamic interfacial tension values obtained by drop volume tensiometry will be affected under certain experimental conditions by the formation of a neck between the drop and the capillary tip. This phenomenon must be accounted for to obtain accurate values of interfacial tension. In this work, neck formation for a water–mineral oil system is studied under conditions where hydrodynamic effects can be neglected. A model originally developed for the determination of the surface tension of a suspended drop is modified for application to dynamic interfacial tensions of surfactant-containing liquids. The model relates apparent values of interfacial tension calculated from drops possessing necks to actual values. Experiments with Span 80 (sorbitan monooleate) and sodium dodecyl sulfate (SDS) surfactants in a mineral oil–water system are used to test the validity of the developed model. For the small tip diameter used, good agreement is obtained for Span 80 up to the critical micelle concentration, and for low concentrations of SDS, when the surfactant adsorption is diffusion-limited. In both cases, the neck diameter of the growing drop can be considered constant over the range of dynamic interfacial tensions tested. 相似文献
67.
X. Y. Wu R. H. Pelton K. C. Tam D. R. Woods A. E. Hamielec 《Journal of polymer science. Part A, Polymer chemistry》1993,31(4):957-962
Conductometric titration of poly(N-isopropylacrylamide) (polyNIPAM) with sodium dodecyl sulfate (SDS) gave two apparent transitions labeled C1 and C2. The C1 transition was independent of polyNIPAM concentration in the 0.05–0.3 wt % range, whereas C2 was proportional to the polymer concentration. C1 corresponded to the onset of binding of surfactant with polymer. Arguments based on a simple mass action model for micellization are presented to show that C2, the second transition, is not due to any simple explanation such as being the point above which only free micelles are formed with surfactant addition. The cloud point of polyNIPAM increased with the amount of bound surfactant. This was attributed to electrostatic contribution of bound sulfate groups to the increased solubility of polyNIPAM. © 1993 John Wiley & Sons, Inc. 相似文献
68.
Ayumi Yoshida Hitoshi Yamauchi Hideki Sakai Norimichi Kawashima Masahiko Abe 《Colloids and surfaces. B, Biointerfaces》1997,8(6):191-342
Molecular interactions between lipid bilayers (liposomes) and chondroitin sulfate C (CS), a water soluble polymer, have been investigated in terms of zeta-potential, particle size, microscopic-viscosity, microscopic-polarity of liposomes and permeability of calcein. Microscopic morphology is dramatically changed by the addition of CS to the positively charged liposomes (Pos.L), while it is not changed by the addition to uncharged liposomes (Unc.L) or negatively charged liposomes (Neg.L). The absolute value of the particle size of Pos.L increases with the addition of CS, while the zeta- potential of Pos.L decreases. Permeability of Pos.L decreases with an increase in the concentration of CS. Phase transition temperature of Pos.L is changed after the addition of CS. These values, however, are not changed for the other liposomes by the addition of CS. The results of gel filtration chromatography show that CS is absorbed on the Pos.L surface. Microscopic viscosity is also increased by the addition of CS to Pos.L due to the adsorption of CS. 相似文献
69.
A. Aagli N. Tamer A. Atbir L. Boukbir M. El Hadek 《Journal of Thermal Analysis and Calorimetry》2005,82(2):395-399
Summary Solubility of calcium sulfate in concentrated aqueous chloride solutions is of particular significance in chloride hydrometallurgy
and various crystallization processes, such as the production of potassium sulfate from phosphogypsum and potassium chloride.
This paper examines an example of the second type of application in which gypsum and potassium chloride are reacted to form
K2SO4. The solubility of phosphogypsum in aqueous solutions of KCl, HCl, and mixtures of both has first been measured at various
temperatures and concentrations. The parameters investigated are HCl concentration up to 6M, KCl concentration up to 180 g
L-1 and temperature from 25 to 80°C. In addition, the influence of co-existing chloride salts, such as (HCl+KCl), on the solubility
of calcium sulfate is estimated from 25 to 80°C. The solubility increases obviously with the temperature increment as it does
initially with acid concentration, reaching a maximum of about 3M HCl, 130 g L-1 KCl and then drops. At the same time, the solubility of CaSO4·2H2O decreases with increasing KCl concentration. 相似文献
70.
AbstractAnilines are rapidly, often within 60?minutes, converted into the corresponding phenols in up to 87% isolated yield. The presented experimentally simple protocol display broad compatibility with a variety of functional groups, and in particular, well suited for the preparation of methyl-substituted phenols. Such phenols are not easily available by other synthetic approaches. The formation of phenolic radical coupling products was not observed even for activated anilines using this open flask method. 相似文献