生物神经网络系统动力学与功能研究

生物神经系统是由数量极其巨大的神经元相互联结的信息网络系统,在生物体的感觉、认知和运动控制中发挥关键性的作用.首先介绍神经元、大脑和一些生物神经网络的生理结构和理论模型,然后分别介绍其放电活动和网络动态特性的一些重要问题,包括神经元的复杂放电模式、耦合神经元网络系统的同步活动和时空动力学、大脑联合皮层神经微回路的网络结构特征,以及工作记忆和抉择过程的动力学机制等. 最后对今后研究给出一些展望.      

Measurement of the choroidal blood flow-time curve and its applications

So far it has been difficult to show up the state of blood flow in choroid due to its position underlying behind the retina anatomically. This paper presents the principle and the method of data analysis in the measurement of the choroidal blood flow-time curve by means of laser interferometry. Preliminary investigations show that this technique may be important in the diagnosis of multiple arteritis and prediction of cerebral ischemic disturbances.     

近红外空间分辨光谱技术及其对新生猪脑缺氧缺血的检测

近红外光谱(NIRS)技术作为一种无创的组织氧检测手段,近年来在脑缺氧缺血的检测方面日益受到重视。文章介绍了自行研制的NIRS仪器(TSAH-100近红外组织血氧无损监测仪)的基本原理及用于新生猪脑氧检测时如何实现传感器与待测脑组织的最佳耦合。检测了28例新生猪在不同氧合状态下的脑组织氧饱和度(regional cerebral oxygen saturation, rSO₂),在缺氧结束后进行了有创的动脉氧饱和度及生理参数的检测。结果表明,NIRS无创测得的脑rSO₂与血气分析有创测得的动脉血氧饱和度(SaO₂)有很好的相关性(p<0.001),并且脑rSO₂与缺氧程度及缺氧后生理参数的变化一致。因此NIRS无创测得的脑rSO₂能直接判断脑氧合状态,可在一定情况下替代有创血气分析,帮助临床无创、简便地诊断脑缺氧缺血。     

Measurement of the choroidal blood flow-time curve and its applications

So far it has been difficult to show up the state of blood flow in choroid due to its position underlying behind the retina anatomically. This paper presents the principle and the method of data analysis in the measurement of the choroidal blood flow-time curve by means of laser interferometry. Preliminary investigations show that this technique may be important in the diagnosis of multiple arteritis and prediction of cerebral ischemic disturbances.     

Protein array for assist diagnosis of acute myocardial infarction

A nanogold probe immunoassay for cardiac troponin I (cTnI) combining the concepts of the one-step dual monoclonal antibody “sandwich” principle, the low density protein array, and silver enhancement on the gold particle is described. Two main substrates, namely the capture antibody (IgG1) coated supporting nitrocellulose membrane and the colloidal gold-labeled detection antibody (cAu–IgG2), were prepared before the detection. The detection procedure involved two steps, i.e. immunoreaction and silver amplification. The assay needs only small amounts of serum samples of patients. The detection results could be easily imaged with a simple flatbed scanner or even the naked eye. The whole detection procedure of the assay could be fulfilled within 40 min (much faster than the routine enzyme-linked immunosorbent assay (ELISA) that takes usually at least 3 h for a turnaround test). The detection limit of cTnI was found to be 1 ng/ml. The detecting results of cTnI in serum samples were similar to those detected by ELISA.     

脑血栓形成患者血清微量元素含量分析

为了解脑血栓形成与微量元素的关系 ,测定了 1 4例脑血栓形成患者和 1 4例健康成人的血清Zn、Cu、Mn、Mg、Cr的含量。结果显示 ,与健康成人相比 ,脑血栓形成患者的Zn、Cr含量下降 (P <0 0 5或P <0 0 1 ) ,ρ(Zn) /ρ(Cu)比值下降 (P <0 0 0 1 )。提示脑血栓形成时血清微量元素水平有一定的改变。采取措施对体内微量元素进行调控 ,应不失为一种防治脑血栓形成的方法     

治疗小儿感染性中毒性脑病的体会

介绍了收治３４例小儿感染性中毒性脑病的体会，应及时足量用山莨菪治疗， 还采用维生素Ｅ、叶酸抗氧剂、保肝药，以及纠酸等代谢紊乱。     

Myocardial infarction patients show altered lipoprotein properties and functions when compared with stable angina pectoris patients

Several parameters and risk factors were compared between Korean male myocardial infarction (MI) patients (n = 10) and angina pectoris (AP) patients (n = 17) to search unique biomarkers for myocardial infarction (MI) in lipoprotein level. Individual serum and lipoprotein fractions (VLDL, LDL, HDL₂, HDL₃) were isolated and analyzed by lipid and protein determination and enzyme assay. The MI group was found to have a 25 and 30% higher serum cholesterol and triacylglycerol (TG) level than the AP group, respectively, however, their body mass index (BMI), LDL-cholesterol (C), HDL-C, and glucose levels fell within the normal range. MI patients were found to have an approximately two-fold higher level of serum IL-6 and an 18% lower serum apoA-I level than that of the AP group. LDL and HDL₂ fraction of the MI group were more enriched with TG than those of AP group. The increased TG was correlated well with the increased level of apoC-III in the same fraction. Cholesteryl ester transfer protein (CETP) activity and protein level were greatly increased in MI patients in the LDL and HDL₃ fractions. MI patients showed more severely oxidized LDL fraction than patients in the AP group, as well as the weakest antioxidant ability of serum. Conclusively, MI patients were found to have unique serum and lipoprotein characteristics including increased IL-6 and TG in serum, with CETP and apoC-III in the LDL and HDL fractions, as well as severely impaired antioxidant ability of HDL.     

儿童脑病高压氧治疗的护理

对30例进行高压氧治疗的脑病患儿给予进舱治疗前、治疗时及治疗后护理,使患儿能安全、顺利完成多个疗程的高压氧治疗,从而得到康复,取得满意的临床效果。     

治疗心脑血管疾病中成药中铜锌比值的含量分析

采用火焰原子吸收分光光度法测定了20种治疗心脑血管疾病中成药物中铜锌含量并计算铜锌比值。结果表明,治疗脑血管疾病中成药中Cu/Zn比治疗心血管疾病中成药中Cu/Zn高,并有含量并计算Cu/Zn一定的差异（P＜0．05）,这为探讨中成药物中微量元素与治疗心脑血管疾病的关系提供了有用的数据,同时也为临床上合理用药提供了微量元素方面的依据。