The combination of polymers with nucleic acids leads to materials with significantly advanced properties. To obviate the necessity and complexity of conjugating two macromolecules, a polymer initiator is described that can be directly covalently linked to DNA during solid‐phase synthesis. Polymer can then be grown from the DNA bound initiator, both in solution after the DNA‐initiator is released from the solid support as well as directly on the solid support, simplifying purification. The resulting polymer‐DNA hybrids were examined by chromatography and fluorescence methods that attested to the integrity of hybrids and the DNA. The ability to use DNA‐based supports expands the range of readily available molecules that can be used with the initiator, as exemplified by direct synthesis of a biotin polymer hybrid on solid‐support. This method expands the accessibility and range of advanced polymer biohybrid materials. 相似文献
The self‐assembly of nanostructured globular protein arrays in thin films is demonstrated using protein–polymer block copolymers based on a model protein mCherry and the polymer poly(oligoethylene glycol acrylate) (POEGA). Conjugates are flow coated into thin films on a poly(ethylene oxide) grafted Si surface, forming self‐assembled cylindrical nanostructures with POEGA domains selectively segregating to the air–film interface. Long‐range order and preferential arrangement of parallel cylinders templated by selective surfaces are demonstrated by controlling relative humidity. Long‐range order increases with coating speed when the film thicknesses are kept constant, due to reduced nucleation per unit area of drying film. Fluorescence emission spectra of mCherry in films prepared at <25% relative humidity shows a small shift suggesting that proteins are more perturbed at low humidity than high humidity or the solution state.
The 3-ethyl thiazolium peptide salts Boc-Thia-Leu-OMe and Boc-Thia-Leu-Phe-OMe based on the unnatural amino acid thiazolylalanine (Thia) have been prepared. After deprotonation, they reacted rapidly via the silver carbene transfer reaction with [RhCp∗Cl2]2 and [Ru(p-cymene)Cl2]2 to yield the corresponding thiazole-based carbene complexes 7-10 in acceptable yield. All new compounds were characterized by multinuclear NMR spectroscopy, mass spectrometry, and elemental analysis. These complexes constitute the first examples of thiazolylalanine-2-ylidene metal bioconjugates. 相似文献
In this paper we developed an entirely new and highly sensitive luminol-H2O2 flow injection chemiluminescence system using the enhancement effect of CdTe quantum dots-IgG bioconjugates. Immunoglobulin G (IgG) as a kind of bio-molecule was conjugated to different sized CdTe semiconductor quantum dots (QDs). Using PL spectra and CL intensity profiles, it was found that chemiluminescence resonance energy transfer (CRET) was possibly occurring between CdTe-IgG bioconjugate and luminol. Under optimum conditions, increase of IgG concentration in CdTe-IgG bioconjugate resulted enhancing effect on CL intensity of luminol-H2O2 system. Moreover quenching effects on CL intensity by addition of different proteases can construct turn off biosensor for these proteases with low detection limits and wide linear range. Furthermore, the effects of various organic and inorganic species on CdTe-IgG bioconjugates enhanced luminol-H2O2 CL system were also studied in this paper. 相似文献
The functionality of natural biopolymers has inspired significant effort to develop sequence‐defined synthetic polymers for applications including molecular recognition, self‐assembly, and catalysis. Conjugation of synthetic materials to biomacromolecules has played an increasingly important role in drug delivery and biomaterials. We developed a controlled synthesis of novel oligomers from hydroxyproline‐based building blocks and conjugated these materials to siRNA. Hydroxyproline‐based monomers enable the incorporation of broad structural diversity into defined polymer chains. Using a perfluorocarbon purification handle, we were able to purify diverse oligomers through a single solid‐phase extraction method. The efficiency of synthesis was demonstrated by building 14 unique trimers and 4 hexamers from 6 diverse building blocks. We then adapted this method to the parallel synthesis of hundreds of materials in 96‐well plates. This strategy provides a platform for the screening of libraries of modified biomolecules. 相似文献