In the last decade, substantial research in the field of post‐plasma grafting surface modification has focussed on the introduction of carboxylic acids on surfaces by grafting acrylic acid (AAc). In the present work, we report on an alternative approach for biomaterial surface functionalisation. Thin poly‐ε‐caprolactone (PCL) films were subjected to a dielectric barrier discharge Ar‐plasma followed by the grafting of 2‐aminoethyl methacrylate (AEMA) under UV‐irradiation. X‐ray photoelectron spectroscopy (XPS) confirmed the presence of nitrogen. The ninhydrin assay demonstrated, both quantitatively and qualitatively, the presence of free amines on the surface. Confocal fluorescence microscopy (CFM), atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to visualise the grafted surfaces, indicating the presence of pAEMA. Static contact angle (SCA) measurements indicated a permanent increase in hydrophilicity. Furthermore, the AEMA grafted surfaces were applied for comparing the physisorption and covalent immobilisation of gelatin. CFM demonstrated that only the covalent immobilisation lead to a complete coverage of the surface. Those gelatin‐coated surfaces obtained were further coated using fibronectin. Osteosarcoma cells demonstrated better cell‐adhesion and cell‐viability on the modified surfaces, compared to the pure PCL films.
The compatible carbon-silicon complex materials originated from precursor diglycerylsilane (DGS) and sugar-modified silane
N-(3-triethoxysilylpropyl)gluconamide (GLS) have gained substantial popularity by demonstrating admirable properties to stabilize
entrapped biomolecules. The microenvironment inside these materials, especially the distribution of sugar moieties inside
the matrix, which is likely the most critical factor determining compatibility of these materials, still remains unclear.
To deeply investigate the biocompatibility mechanism of these materials, we have adopted two different preparation routes
for these materials by introducing GLS into the starting DGS sol stage, but things are different after the DGS gel is formed.
A fluorescence probe rhodamine 6G is introduced herein in the DGS sol to monitor the distribution of GLS moieties, as well
as the evolution of the microenvironment inside resulting materials. All in all, the findings demonstrated that the timing
of GLS addition plays a critical role in controlling the evolution of the inner structure of materials, suggesting that this
factor provides a promising route to tune the properties of the resulting materials.
Supported by the National Natural Science Foundation of China (Grant No. 20876176), Scientific Research Foundation for the
Returned Overseas Chinese Scholars by the State Education Ministry, Key Project of Chinese Ministry of Education (Grant No.
109100), Doctoral Project of Shandong Province (Grant No. 2008BS09013), Research Foundation of Key Laboratory of Carbon Materials,
Institute of Coal Chemistry, CAS (Grant No. KFJJ0506), and Natural Science Foundation of Shandong Province (Grant No. Q2007B02) 相似文献
In this work, a series of PLGA‐PEG diblock copolymers were synthesized by ring‐opening polymerization of L‐lactide and glycolide using mPEG as macroinitiator and stannous octoate as catalyst. Spherical micelles were obtained from the various copolymers by using co‐solvent evaporation method. The biocompatibility of micelles was evaluated with the aim of assessing their potential in the development of drug delivery systems. Various aspects of biocompatibility were considered, including MTT assay, agar diffusion test, release of cytokines, hemolytic test, dynamic clotting time, protein adsorption in vitro, and zebrafish embryonic compatibility in vivo. The combined results revealed that the micelles present good cytocompatibility and hemocompatibility in vitro. Moreover, the cumulative effects of micelles throughout embryos developing stages have no toxicity in vivo. It is thus concluded that micelles prepared from PLGA‐PEG copolymers present good biocompatibility as potential drug carrier. 相似文献
The use of carboxylate side chains to induce peptide helicity upon binding to dirhodium centers is examined through experimental and computational approaches. Dirhodium binding efficiently stabilizes α helicity or induces α helicity in otherwise unstructured peptides for peptides that contain carboxylate side chains with i, i+4 spacing. Helix induction is furthermore possible for sequences with i, i+3 carboxylate spacing, though in this case the length of the side chains is crucial: ligating to longer glutamate side chains is strongly helix inducing, whereas ligating the shorter aspartate side chains destabilizes the helical structure. Further studies demonstrate that a dirhodium metallopeptide complex persists for hours in cellular media and exhibits low toxicity toward mammalian cells, enabling exploitation of these metallopeptides for biological applications. 相似文献
Biocomposite layers of silica and various bone-relevant proteins such as collagen, gelatine and commercial collagen hydrolysate can be obtained from coatings of silica sols mixed with proteins in water/dioxane. Investigations into the mechanical and cell proliferation properties for different sol parameters (pH, solvent), type and concentration of proteins, annealing and crosslinking of the biocomposite layers revealed that such coatings are highly biocompatible with excellent mechanical properties. 相似文献
The biodegradation rate and biocompatibility of poly (d, / -lactide) (PDLLA)in vivo were evaluated. The aim of this study was to establish a nerve guide constructed by the PDLLA with 3-D microenvironment and
to repair a 10 mm of sciatic nerve gap in rats. The process of the nerve regeneration was investigated by histological assessment,
electrophysiological examination, and determination of wet weight recovery rate of the gastrocnemius muscle. After 3 weeks,
the nerve guide had changed from a transparent to an opaque status. The conduit was degraded and absorbed partly and had lost
their strength with breakage at the 9th week of postoperation. At the conclusion of 12 weeks, proximal and distal end of nerves
were anastomosed by nerve regeneration and the conduit vanished completely. The results suggest that PDLLA conduits may serve
for peripheral nerve regeneration and PDLLA is a sort of hopeful candidate for tissue engineering. 相似文献