UV-B辐射对2种海洋微藻生长和生理生化特征的影响

通过实验生态学和生物化学的方法,研究了UV-B辐射对杜氏盐藻、小角毛藻的生长、叶绿素含量、可溶性蛋白含量、超氧化物歧化酶活力的影响.结果表明：（1）UV-B辐射对小角毛藻的生长起促进作用,高剂量UV-B辐射对盐藻的生长具有一定抑制作用.（2）2种微藻叶绿素含量均低于对照组,UV-B辐射使盐藻叶绿素含量先下降后升高,而小角毛藻则随辐射剂量的增加呈现下降趋势.（3）2种微藻可溶性蛋白含量均低于对照组,且随辐射剂量的增加而下降;2种微藻超氧化物歧化酶活力较对照组呈现出升高的趋势.     

一类生化反应方程的分枝

文[1]建立了一类生化反应方程E_(α,β),该文借助于计算机作了极限环的一些数值计算。[2]讨论了E_(σ,β)的极限环的不存在性,存在性和唯一性。本文弄清楚了E_(α,β)(α≥0,β>0)的各种分枝,包括高阶奇点分枝,各阶Hopf分枝,同宿轨道分枝,半稳定环分枝。如果还假定至多只有2个极限环,刚半稳定环分枝还是唯一的(详见定理A)。     

Risk Assessment in Monitoring of Water Analysis of a Brazilian River

This study aimed to introduce non-parametric tests and guard bands to assess the compliance of some river water properties with Brazilian environmental regulations. Due to the heterogeneity of the measurands pH, Biochemical Oxygen Demand (BOD), manganese molar concentration, and Escherichia coli, which could be wrongly treated as outliers, as well as the non-Gaussian data, robust methods were used to calculate the measurement uncertainty. Next, based on guard bands, the compliance assessment was evaluated using this previous uncertainty information. For these four measurands, partial overlaps between their uncertainties and the specification limit could generate doubts about compliance. The non-parametric approach for calculating the uncertainty connected to the guard bands concept classified pH and BOD as “conform”, with a risk to the consumer of up to 4.0% and 4.9%, respectively; in contrast, manganese molar concentration and Escherichia coli were “not conform”, with a risk to the consumer of up to 25% and 7.4%, respectively. The methodology proposed was satisfactory because it considered the natural heterogeneity of data with non-Gaussian behavior instead of wrongly excluding outliers. In an unprecedented way, two connected statistical approaches shed light on the measurement uncertainty in compliance assessment of water analysis.     

基于激光阵列的多通道微梁生化传感系统

针对单微悬臂梁生化检测系统中存在的温度漂移、溶液折射率变化等环境噪声影响和不能多目标检测等问题,设计制作了一种基于垂直腔面射型激光器阵列(VCSELs)的新型微悬臂梁阵列生化传感系统。利用双透镜组光路汇聚激光器阵列发出的激光束,依次照射到微悬臂梁阵列尖端进行精确定位扫描,实现了5根微悬臂梁变形信号的同步检测。该系统具有灵敏度高、一致性好、检测快速等优点。通过升降温动态实验测试,验证了该系统的稳定可靠性。上述结果为微悬臂梁阵列式生化传感技术的开展提供了一种新的方法和平台。     

基于NMR的代谢组学方法对硝酸钕急性生物效应的研究

采用现代核磁共振和模式识别技术,分析了腹腔注射给药2、10和50mg/kg体重剂量硝酸钕48h内Wistar大鼠尿液和血清的核磁共振氢谱。由尿液及血清中内源性代谢物如柠檬酸、肌酸酐、N-氧三甲胺、氨基酸、乳酸、琥珀酸、牛磺酸及葡萄糖等物种的浓度变化,结合大鼠血清指标和肝、肾组织切片图研究了轻稀土化合物Nd(NO3)3在大鼠体内的急性生物效应。结果表明,3个剂量的Nd(NO3)3主要对大鼠肝脏造成了不同程度的损伤,而且随着剂量的升高渐趋严重。同时,Nd(NO3)3也对肾脏的特定部位(肾乳头、肾小管)造成了损害。     

水包水型阳离子乳液絮凝剂的合成及其应用

以丙烯酰胺(AM)和丙烯酰氧乙基三甲基氯化铵(DAC)为主要单体在聚乙二醇20000(PEG 20000)溶液中进行自由基共聚合成了固含量高、絮凝效果好且速溶的水包水型阳离子聚丙烯酰胺乳液。在充氮气30 min,60℃下反应5 h,AM和DAC质量百分数分别为20%和0.5%的最优条件下,合成的阳离子絮凝剂(CPF)乳液对生化污水上清液透光率达到95%。红外光谱分析表明,聚合物分子结构中含有AM和DAC链节。     

Temporal Control of Gelation and Polymerization Fronts Driven by an Autocatalytic Enzyme Reaction

Chemical systems that remain kinetically dormant until activated have numerous applications in materials science. Herein we present a method for the control of gelation that exploits an inbuilt switch: the increase in pH after an induction period in the urease‐catalyzed hydrolysis of urea was used to trigger the base‐catalyzed Michael addition of a water‐soluble trithiol to a polyethylene glycol diacrylate. The time to gelation (minutes to hours) was either preset through the initial concentrations or the reaction was initiated locally by a base, thus resulting in polymerization fronts that converted the mixture from a liquid into a gel (ca. 0.1 mm min^?1). The rate of hydrolytic degradation of the hydrogel depended on the initial concentrations, thus resulting in a gel lifetime of hours to months. In this way, temporal programming of gelation was possible under mild conditions by using the output of an autocatalytic enzyme reaction to drive both the polymerization and subsequent degradation of a hydrogel.     

肾上腺皮质疾病生化检验诊断价值

目的对肾上腺皮质疾病生化检验诊断价值进行探析。方法收集广东省中山市三角医院在2013年1月—2015年6月收治的60例肾上腺皮质疾病患者,分为两组,分别使用常规检验与生化检验进行诊断,对比与观察两组患者的检出率。结果观察组患者的检验有效率是90%,对照组是70%,差异性显著(P0.05)。结论生化检验在肾上腺皮质疾病临床诊断中的应用,具备良好的诊断价值,能够提高患者的检验有效率,提高临床诊断与检验结果的符合率,以准确判断患者疾病,并以此为基础制定科学有效的治疗方案,提高患者的临床治疗效果,改善患者预后。     

基于微流控驱动和控制技术的临床生化分析系统研究进展

微流控技术具有微量、高效、高通量、微型化、集成化、自动化的特点，为实现现场化、低成本的临床生化分析提供了一条可行的技术途径。针对于目前临床生化分析中复杂流体操控的难点，该文依据系统中采用驱动和控制方式的不同，对各种基于微流控技术的临床生化分析系统进行了分类介绍，同时也介绍了市场上商品化的微流控生化分析仪器，并对其各自特点进行了综合评述。