摄食转牙鲆生长因子基因集胞藻的大菱鲆血液指标及微核研究

以实验室养殖的幼小大菱鲆和养殖场养殖大菱鲆幼鱼为研究对象，研究了转牙鲆生长因子基因集胞藻作为饲料添加剂对鱼类血液生理生化指标和红细胞微核率及核异常率的影响。研究结果显示，转基因集胞藻对大菱鲆血液红细胞和白细胞数量、血红蛋白含量及红细胞体积没有影响（p〉0.05）；1.0％添加剂量没有引起血清转氨酶活性及碱性磷酸酶和乳酸脱氢酶活性增加（p〉0.05），实验组血清蛋白质、葡萄糖、甘油三脂、胆固醇、尿素氮和肌酐含量以及钾、钠、氯、磷浓度与对照组之间也不存在显著性差异（p〉0.05）；转基因集胞藻对红细胞微核及核异常没有诱导作用。结果表明转基因集胞藻不会引起大菱鲆组织器官损伤，也没有遗传毒性，是一种安全的转基因饲料添加剂，可以用作大菱鲆促生长剂使用。     

生物化学反应中一类非线性系统极限环的存在惟一性

研究了生物化学反应中一类非线性系统，得到了该系统的环绕正奇点极限环的充分必要条件，并且证明了如果存在极限环，则必惟一。     

The Holographic Principle for Latent Molecular Properties

All information about all latent molecular properties, not exhibited by a given molecular structure but reproducibly exhibited by the same molecule in a different state or having a different conformation, is fully encoded in any nonzero volume of the non-degenerate ground state electron density.     

不同发育阶段大鼠NaF与血清生化和元素含量慢性反应的研究

不同日龄３批雄性大鼠饲氟浓度５３．２＊１０＾－６,实验３个月。结果表明,与同日龄正常对照组相比,杆、肾铝显著升高的断乳２周大鼠的血清钙,ＲＢＣ、Ｈｂ含量、红细胞平均血红蛋白量、全血锌,全血铁,骨钙,骨磷含量均出现显著变化。２８０日龄且的体重和全血磷受影响较为显著。     

Microfluidic-based single cell trapping using a combination of stagnation point flow and physical barrier

Single cell trapping in vitro by microfluidic device is an emerging approach for the study of the rela-tionship between single cells and their dynamic biochemical microenvironments. In this paper, a hydrodynamic-based microfluidic device for single cell trapping is designed using a combination of stagnation point flow and physical barrier. The microfluidic device overcomes the weakness of the tra-ditional ones, which have been only based upon either stag-nation point flows or physical barriers, and can conveniently load dynamic biochemical signals to the trapped cell. In addi-tion, it can connect with a programmable syringe pump and a microscope to constitute an integrated experimental system. It is experimentally verified that the microfluidic system can trap single cells in vitro even under flow disturbance and con-veniently load biochemical signals to the trapped cell. The designed micro-device would provide a simple yet effective experimental platform for further study of the interactions between single cells and their microenvironments.     

Label-free and rapid spectroscopic evaluation of ripening of Syzygium cumini fruit

In the present investigation, attempts have been made to obtain the spectral signature of the fruit jamun and to estimate the changes in biochemical composition that take place in the process of ripening of the fruits. The changes in exocarp, mesocarp, and seed of the fruit in raw and ripe stages have been investigated with the help of label-free, nondestructive, and rapid techniques: laser-induced fluorescence and attenuated total reflectance Fourier transform infrared spectroscopy. For this, the fluorescence spectra of the different parts (exocarp, mesocarp, and seed) of the raw and ripe fruits of jamun excited by 405?nm violet diode laser have been recorded in the spectral region 400–900?nm. The spectrum shows the spectral signature of anthocyanin, flavonoids, carotenoid, and chlorophyll. The observed decrease in the intensity of chlorophyll bands and simultaneous increase in the intensity of anthocyanin and carotenoid bands is regarded as indicator of the ripening of the fruit of jamun. Also, attenuated total reflectance Fourier transform infrared spectra have been recorded in the spectral region 4000–485?cm^?1 for the identification of functional groups associated with different biochemical altered during ripening process of the fruits of jamun. The analyses of the recorded infrared spectra show the presence of carbohydrates, cell wall components, phenolic compounds, nucleic acid, and amide. The quantitative estimation of the biochemical content in the different parts of jamun fruit during the ripening process has been done through calculating the area of the band by curve fitting. The determined spectral signatures can be utilized for the effective monitoring of jamun fruit.     

Risk Assessment in Monitoring of Water Analysis of a Brazilian River

This study aimed to introduce non-parametric tests and guard bands to assess the compliance of some river water properties with Brazilian environmental regulations. Due to the heterogeneity of the measurands pH, Biochemical Oxygen Demand (BOD), manganese molar concentration, and Escherichia coli, which could be wrongly treated as outliers, as well as the non-Gaussian data, robust methods were used to calculate the measurement uncertainty. Next, based on guard bands, the compliance assessment was evaluated using this previous uncertainty information. For these four measurands, partial overlaps between their uncertainties and the specification limit could generate doubts about compliance. The non-parametric approach for calculating the uncertainty connected to the guard bands concept classified pH and BOD as “conform”, with a risk to the consumer of up to 4.0% and 4.9%, respectively; in contrast, manganese molar concentration and Escherichia coli were “not conform”, with a risk to the consumer of up to 25% and 7.4%, respectively. The methodology proposed was satisfactory because it considered the natural heterogeneity of data with non-Gaussian behavior instead of wrongly excluding outliers. In an unprecedented way, two connected statistical approaches shed light on the measurement uncertainty in compliance assessment of water analysis.     

生物酶振荡器研究谷胱甘肽的抗氧化效应

组装了一种生物酶振荡器（Peroxidase-Oxidase Biochemical Oscillator） 的实验装置、建立了相应的实验方法,在此基础上得到单倍周期、四倍周期、混沌 等丰富的振荡图谱信息,并采用它作为“探针”,检测了谷胱甘肽（GSH）的抗氧 化效应,它可作为定量分析的基础,线性范围为9.38 * 10~(-7) ～ 7.50 * 10~(- 5) mol·L~(-1),探讨了某些可能的反应机理。     

Forensic Biochemical Markers to Evaluate the Agonal Period: A Literature Review

Currently, forensic research is multidisciplinary with new methods and parameters useful to define the cause and time of death as well as survival/agony times. The identification of biochemical markers able to estimate agonal period has been studied by many forensic researchers. It is known that the estimation of agonal time in different types of death is not always easy, hence our interest in literature’s data. The studies analyzed in this review confirm the important role of thanatobiochemistry for the estimation of survival times. Regardless of the death cause, the survival/agony time between the primary event and death influences markers concentrations in biological samples (e.g., blood, urine, cerebrospinal fluid). Different biomarkers can be used for qualitative evaluations in deaths with short and long agony (e.g., C-reactive protein, ferritin, GFAP, etc.). Instead, the quantitative interpretation showed limits due to the lack of reference cut-offs. Thanatobiochemistry is a useful tool to confirm what emerged from autopsies findings (macroscopic and histological analysis), but further studies are desirable to confirm the evidence emerging from our review of the literature.     

基于激光阵列的多通道微梁生化传感系统

针对单微悬臂梁生化检测系统中存在的温度漂移、溶液折射率变化等环境噪声影响和不能多目标检测等问题,设计制作了一种基于垂直腔面射型激光器阵列(VCSELs)的新型微悬臂梁阵列生化传感系统。利用双透镜组光路汇聚激光器阵列发出的激光束,依次照射到微悬臂梁阵列尖端进行精确定位扫描,实现了5根微悬臂梁变形信号的同步检测。该系统具有灵敏度高、一致性好、检测快速等优点。通过升降温动态实验测试,验证了该系统的稳定可靠性。上述结果为微悬臂梁阵列式生化传感技术的开展提供了一种新的方法和平台。