全文获取类型
收费全文 | 243篇 |
免费 | 27篇 |
国内免费 | 32篇 |
专业分类
化学 | 165篇 |
力学 | 5篇 |
综合类 | 15篇 |
数学 | 56篇 |
物理学 | 61篇 |
出版年
2023年 | 3篇 |
2022年 | 15篇 |
2021年 | 13篇 |
2020年 | 8篇 |
2019年 | 7篇 |
2018年 | 6篇 |
2017年 | 11篇 |
2016年 | 13篇 |
2015年 | 9篇 |
2014年 | 17篇 |
2013年 | 21篇 |
2012年 | 9篇 |
2011年 | 16篇 |
2010年 | 18篇 |
2009年 | 12篇 |
2008年 | 18篇 |
2007年 | 22篇 |
2006年 | 13篇 |
2005年 | 15篇 |
2004年 | 8篇 |
2003年 | 10篇 |
2002年 | 6篇 |
2001年 | 10篇 |
2000年 | 6篇 |
1999年 | 6篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
排序方式: 共有302条查询结果,搜索用时 15 毫秒
51.
52.
《Analytical letters》2012,45(2-3):227-241
ZnSe quantum dots (QDs) that were capped with 11-mercaptoundecanoic acid (MUA) and conjugated to amino-modified ssDNA molecules exhibited variations in fluorescence emission intensity upon hybridization with complementary ssDNA in solution, a phenomenon that can be exploited for rapid detection of free ssDNA sequences. Conjugation of MUA-capped ZnSe QDs to amino-modified ssDNA molecules resulted in increased fluorescence emission intensity and stability at room temperature. Increasing the length of the ssDNA, that was conjugated to the QDs, resulted in increased fluorescence emission intensity up to a length of about 50 nucleotide bases, beyond which the peak emission intensity reached a plateau. Hybridization of QD-ssDNA conjugates with complementary ssDNA, either in free form or bound to QDs from the same population, resulted in additional fluorescence emission intensity amplification. A small red shift was observed when three-dimensional QD-dsDNA-QD structures were formed. The QD-ssDNA sensors with single ssDNA molecule per QD were developed and used for rapid quantitative detection of fully or partially complementary free ssDNA sequences in aqueous solution. Partial hybridization of the QD-ssDNA sensors with short ssDNA targets resulted in smaller QD emission intensity amplification, when compared to full hybridization. A QD-ssDNA sensor containing a sequence corresponding to the hemoglobin beta gene was used to detect and discriminate between free ssDNA targets consisting of a complementary ssDNA sequence and targets containing a single-base mutation that can cause sickle-cell anemia. Such QD-based biosensors can form the basis for rapid separation-free assays that can be used to detect target biomolecules in solution. 相似文献
53.
卫星搭载紫花苜蓿种子的拉曼光谱分析 总被引:1,自引:0,他引:1
为了了解卫星搭载对紫花苜蓿种子化学特性的影响,揭示空间诱变机理,文章利用傅里叶变换拉曼光谱法对卫星搭载当代的紫花苜蓿种子进行了研究。结果表明,与地面对照相比,飞行组在358和553 cm-1处的峰强增加,814,1 122,1 531,1 743 cm-1处的峰强降低,通过谱带归属发现,增强的358和553 cm-1分别与DNA和Ca2+有关,降低的4个峰与糖类、脂类有关。这说明经过卫星搭载后,苜蓿种子的DNA和Ca2+的量出现增加趋势,糖类与脂类的量出现降低趋势。其可能原因是(1)种子主动修复诱变产生的DNA损伤时,消耗部分贮存能量所致;(2)空间飞行过程中的超重导致种子细胞内的Ca2+ 浓度升高;(3)飞行因子导致种子提前萌发,DNA大量合成与复制,种子储藏的能量提前降解消耗。这一结果将对苜蓿空间诱变机理研究有重要参考价值。 相似文献
54.
为检验人尿胰蛋白酶抑制剂(UTI)两个结构域中的盐键的稳定性对UTI热变性可逆性的影响,将UTI中的Lys121突变成Ile以中断Glu69与Lys121之间形成的盐键,并命名为?UTI.然后对?UTI进行表达纯化和热解折叠实验.结果显示,?UTI在大肠杆菌中成功表达,纯化复性后经园二色谱测定三级结构与UTI基本相似,酶学测定表明恢复胰蛋白酶抑制剂活性.热变性实验表明:在pH低于7.4的缓冲溶液中?UTI的热解折叠是可逆的,与UTI的结果(pH<4.2)相比,pH值提高了3.结合以前对UTI在酸性条件下可逆热解折叠而在中性与碱性条件下不可逆热解折叠的机制的研究结果,认为多结构域蛋白解折叠的曲线除考虑各个结构域单独形成的多个S曲线的叠加外,还要考虑到结构域之间相互作用强度的影响. 相似文献
55.
56.
The mutation operation is critical to the success of genetic algorithms since it diversifies the search directions and avoids convergence to local optima. The earliest genetic algorithms use only one mutation operator in producing the next generation. Each problem, even each stage of the genetic process in a single problem, may require appropriately different mutation operators for best results. Determining which mutation operators should be used is quite difficult and is usually learned through experience or by trial-and-error. This paper proposes a new genetic algorithm, the dynamic mutation genetic algorithm, to resolve these difficulties. The dynamic mutation genetic algorithm simultaneously uses several mutation operators in producing the next generation. The mutation ratio of each operator changes according to evaluation results from the respective offspring it produces. Thus, the appropriate mutation operators can be expected to have increasingly greater effects on the genetic process. Experiments are reported that show the proposed algorithm performs better than most genetic algorithms with single mutation operators. 相似文献
57.
Effect of the mutation of carotenoids on the dynamics of energy transfer in light-harvesting complexes (LH2) from Rhodobacter sphaeroides 601 at room temperature
下载免费PDF全文
![点击此处可从《中国物理》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Energy transfers in two kinds of peripheral light-harvesting complexes (LH2) of
{Rhodobacter sphaeroides} (RS) 601 are studied by using femtosecond pump--probe
spectroscopy with tunable laser wavelength at room temperature. These two complexes
are native LH2 (RS601) and green carotenoid mutated LH2 (GM309). The obtained
results demonstrate that, compared with spheroidenes with ten conjugated double
bonds in native RS601, carotenoid in GM309 containing neurosporenes with nine
conjugated double bonds can lead to a reduction in energy transfer rate in the
B800-to-B850 band and the disturbance in the energy relaxation processes within the
excitonic B850 band. 相似文献
58.
A silent mutation or sequence polymorphism, A to T substitution at codon 399 in exon11 of the PAH gene from a Chinese PKU patient, was found by sequence analysis. The fre-quencies of this new mutation in normal and abnormal (PKU) genes were 0.005 and 0.09,respectively, based on the analyses of 100 normal individuals and 39 PKU patients usingDNA amplification with polymerase chain reaction (PCR) and oligonucleotide hybridizationmethods. This silent mutation can be used as a "genetic marker" for PKU prenatal diagno-sis. Recently, a fetus at risk for PKU, who could not be completely predicted by RFLPslinkage analysis, was prenatally diagnosed with this genetic marker. 相似文献
59.
In this paper, we described an assay for the detection of the C677T mutation in the methylenetetrahydrofolate reductase (MTHFR) gene using denaturing high-performance liquid chromatography (DHPLC). The conditions for DHPLC analysis were systematically investigated based on a general HPLC instrument (Prostar VARIAN). A 225 bp DNA fragment covering the 677 site of MTHFR gene was amplified by PCR technology using the purified DNA from whole blood or whole blood as template DNA. PCR products were directly injected without the need for purification. The C677T mutation could be clearly distinguished by DHPLC technology. Our data demonstrated that DHPLC was a powerful and alternative tool for detection of genetic variants and single-nucleotide polymorphisms to electrophoresis technology. 相似文献
60.
Recently, we proposed a 2D graphical representation of DNA sequence [J Comput Chem 25(2004) 1364-1368]. Based on this representation, we outline one approach to search optimal alignment. We also can judge the mutations between bases and an unknown sequence based on its graph and a known sequence's graph. 相似文献