原子荧光光谱法直接测定二甲基二硒

硒位于第六主族,为准金属元素。在自然界中硒的存在形式按其结合形态分为无机硒和有机硒[1,2]。有机硒较无机硒具有更高的生物活性,因此准确测定各种样品中的有机硒具有重要意义。对于挥发性有机硒,一般采用洗脱富集将其与样品基体分离,采用固相吸附[3,4]或液氮冷阱捕集[5,6],热解析或溶剂萃取后用气相色谱测定[7,8]。这些方法可以提供许多有机硒的信息,但其不足之处是程序较为繁琐。原子荧光光谱分析法(AFS)具有准确度高、灵敏度高、线性范围宽、干扰少等优点,故本文利用AFS的特点及二甲基二硒(DMDSe)的挥发性,在不加任何试剂的条件下,…     

Effects of Cations on HPTS Fluorescence and Quantification of Free Gadolinium Ions in Solution; Assessment of Intracellular Release of Gd3+ from Gd-Based MRI Contrast Agents

8-Hydroxypyrene-1,3,6-trisulfonate (HPTS) is a small, hydrophilic fluorescent molecule. Since the pKa of the hydroxyl group is close to neutrality and quickly responds to pH changes, it is widely used as a pH-reporter in cell biology for measurements of intracellular pH. HPTS fluorescence (both excitation and emission spectra) at variable pH was measured in pure water in the presence of NaCl solution or in the presence of different buffers (PBS or hepes in the presence or not of NaCl) and in a solution containing BSA. pKa values have been obtained from the sigmoidal curves. Herein, we investigated the effect of mono-, di-, and trivalent cations (Na⁺, Ca²⁺, La³⁺, Gd³⁺) on fluorescence changes and proposed its use for the quantification of trivalent cations (e.g., gadolinium ions) present in solution as acqua-ions. Starting from the linear regression, the LoD value of 6.32 µM for the Gd³⁺ detection was calculated. The effects on the emission were also analyzed in the presence of a combination of Gd³⁺ at two different concentrations and the previously indicated mono and di-valent ions. The study demonstrated the feasibility of a qualitative method to investigate the intracellular Gd³⁺ release upon the administration of Gd-based contrast agents in murine macrophages.     

Evolution of Er^3＋ Distribution in the Oxyfluoride Glass Ceramics with Heating

The distribution characteristics of Er3+ ions doped in the oxyfluoride glass ceramics containing LaF3 nanocrystals heat-treated at 650 ℃ for different durations were investigated. The results of the integral absorption cross-section analysis demonstrated that the partition fraction of Er3+ in LaF3 nanocrystals increases with prolonging of heating time. The anomalous phenomena of Er3+ emissions in the up-and the down-conversion fluorescence spectra are well explained based on the calculated results.     

Synthesis, Structure and Luminescence of a Novel 2D Cadmium Coordination Polymer with a Ligand Generated in situ

The hydrothermal reaction of 1,2-dicyanobenzene with NaN3 in the presence of Cd(NO3)2 affords a novel 2D cadmium tetrazolyl-benzoate,{Cd(tzbz)(H2O)}n (H2tzbz is 2-(5-tetrazolyl)-benzoate). The tzbz ligand is generated in situ through the 2+3 Sharpless cycloaddition reaction and hydrolyzation. Its crystal structure was determined by single-crystal X-ray diffraction method. The crystal crystallizes in the orthorhombic system,space group Pbca with a=9.6659(19),b=7.6366(15),c=25.964(5) ,V=1916.5(7) 3,Z=8,Mr=318.57,Dc= 2.208 g/cm3,F(000)=1232 and μ=2.276 mm-1. The Cd(Ⅱ) atom is coordinated by four tzbz ligands and one water molecule to form a severely distorted pentangle bipyramid. While each tzbz ligand connects to four Cd(Ⅱ) atoms in a μ4-η1,η2,η1,η1,η1 coordination mode to construct a 2D architecture of the title complex. Additionally,the title complex exhibits strong fluorescence at room temperature in the solid state.     

Growth and Spectral Properties of Yb^3＋-doped Ba3Gd（BO3）3 Crystal

Crystal of Yb3+-doped Ba3Gd(BO3)3 has been grown by the Czochralski method. The spectroscopic characterizations have been investigated at room temperature. The Yb3+:Ba3Gd(BO3)3 crystal exhibits broad absorption at 976nm with FWHM of 7nm and large overall spitting of 2F7/2 manifold (823cm-1). The absorption and emission cross sections are 5.09×10-21cm2 at 976nm and 0.97×10-21cm2 at 1040nm,respectively. The fluorescence lifetime is 2.84 ms.     

Studies on Monodispersed Microspheres of Zinc Sulfide Doped with Mn^2＋

In this paper,zinc acetate,manganese acetate and thiacetamide are used as raw materials to successfully synthesize monodispersed ZnS:Mn2+ microspheres by using hydrothermal method and taking P123 surfactant as a template. The products were characterized by XRD,STEM,FT-IR and N2 adsorption-desorption. And the results show that the diameter of this microsphere is 1.0 μm or so,which is larger than that of ZnS microsphere without Mn2+ doping,and it has monodispersion,smooth surface and uniform size. The doping of Mn2+ does not obviously change the structure of monodispersed ZnS microsphere. The photoluminescence peak lies in a wide band ranging from 450 to 650 nm,and the microspheres emit orange light; with the increase of Mn2+ concentration,fluorescence intensity of ZnS:Mn2+ microsphere changes,and when the mole ratio of Mn2+:Zn2+ is 0.3:1,the fluorescence intensity is the strongest.     

石墨消解-原子荧光光谱法测定土壤中的总硒

为准确定量土壤硒总量,提出以逆王水(1+1)-石墨消解法消解土壤,氢化物原子荧光光谱法(HG-AFS)测定土壤总硒含量的方法.其中,对消解方式、消解时间和仪器条件进行了探讨,确定最优检测条件.称取0.2 g土壤样品加入5 mL逆王水(1+1),于石墨消解仪120℃消解1.5 h,冷却至室温后用超纯水定容至25 m L,...     

热激活延迟荧光材料的光物理行为及性能预测

热激活延迟荧光(Thermally activated delayed fluorescence, TADF)材料由于三线态激子可通过反系间窜越(Reverse intersystem crossing, RISC)转换为单线态激子,在有机发光二极管(Organic light-emitting diodes, OLEDs)中理论上可达到100%的激子利用率而被广泛关注。但实验上开发设计高性能TADF材料较为复杂且研究周期较长,理论研究可以从本质上建立材料结构-性能的关系,预测材料的性质并提供一定的分子设计策略。本文围绕高性能TADF材料的开发,从发光原理出发,系统阐述了分子的设计策略及光物理参数如材料单-三线态能级差(Single-triplet energy gap,ΔE_ST)、系间/反系间窜越速率、吸收/发射光谱、辐射/非辐射速率等的计算原理、计算方法和研究进展。最后我们探讨了TADF材料理论研究面临的机遇和挑战,通过对TADF材料的理论研究综述和研究前景的展望,期待吸引更多的研究工作者,推动该领域的发展和突破。     

氮中二氧化硫标准气体定值方法的研究

介绍用脉冲荧光分析仪对氮中二氧化硫标准气体定值的分析方法,对方法的精密度、准确度及定值的不确定度进行了考察。该方法的定值结果与重量法的配制值相一致。用该法对荷兰国家计量院制备的氮中二氧化硫标准气体进行比对试验,取得了良好的等效性。     

变温下荧光猝灭和加强理论公式合理性的比较

通过荧光和紫外方法研究盐酸左氧氟沙星、氟罗沙星、加替沙星和沃氟沙星四种喹诺酮类药物与人血清白蛋白(HSA)的作用, 在不同温度下对荧光加强和猝灭理论公式的等价性和精细差异进行了比较, 结果表明: 在不同温度下两种理论公式尽管在求取解离常数上所得结果都可视为有效, 但猝灭方程的双倒数图反而不及荧光加强方程的双倒数图与体现动态、静态猝灭机理的猝灭曲线一致. 对于能量转移效率E, 我们首次定义: 按自由荧光体白蛋白的荧光强度F₀和加入药物至荧光不再变化时的荧光强度F值计算F/F₀, 进而将F/F₀代入E＝１－F/F₀计算能量转移效率E. 它标示出每个体系发生能量转移的最大限度, 向体系中再添加药物, 已不能发生进一步的猝灭, 这一新定义的引入, 使得能量转移效率E这一量值有可能推广到估算白蛋白运载药物能力的最大限度.