3D Multilayered paper‐ and thread/paper‐based microfluidic devices for bioassays

In this paper we describe the fabrication of novel 3D microfluidic paper‐based analytical devices (3D‐μPADs) and a 3D microfluidic thread/paper‐based analytical device (3D‐μTPAD) to detect glucose and BSA through colorimetric assays. The 3D‐μPAD and 3D‐μTPAD consisted of three (wax, heat pressed wax‐printed paper, single‐sided tape) and four (hole‐punched single‐sided tape, blank chromatography circles, heat‐pressed wax‐printed paper, hole‐punched single‐sided tape containing trifurcated thread) layers, respectively. The saturation curves for each assay were generated for all platforms. For the glucose assay, a solution of glucose oxidase (GOx), horseradish peroxidase, and potassium iodide was flowed through each platform and, upon contact with glucose, generated a yellow‐brown color indicative of the oxidation of iodide to iodine. For the protein assay, BSA was flowed through each device and, upon contact with citrate buffer and tetrabromophenol blue, resulted in a color change from yellow to blue. The devices were dried, scanned, and analyzed yielding a correlation between either yellow intensity and glucose concentration or cyan intensity and BSA concentration. A similar glucose assay, using unknown concentrations of glucose in artificial urine, was conducted and, when compared to the saturation curve, showed good correlation between the theoretical and actual concentrations (percent differences <10%). The development of 3D‐μPADs and 3D‐μTPADs can further facilitate the use of these platforms for colorimetric bioassays.     

Semiconducting polymer dots as near‐infrared fluorescent probes for bioimaging and sensing

In recent years, semiconducting polymer dots (Pdots) have emerged as a new type of ultrabright fluorescent probes, which have been proved to be very useful for biomedical imaging. Pdots possess several exceptional advantages including high fluorescence brightness, fast radiative rate, excellent photostability, and negligible cytotoxicity. Among these new types of Pdots, the near‐infrared (NIR) fluorescent Pdots appear to be the most urgent and important owing to their promising deep‐tissue imaging in the clinic. This mini‐review highlights the recent progress in the design of NIR‐emitting Pdots and their biomedical applications both in vitro and in vivo.     

Particulate matter (PM10) from São Carlos-SP (Brazil): spectroanalytical techniques to evaluate and determine chemical elements

Chemical composition of PM10 was studied during the period of 2014–2015 in the city of São Carlos, Brazil (‘Dos Voluntários’ Square). PM10 samples were directly analysed by wavelength dispersive X-ray fluorescence – WD-XRF (Al, Ca, Ce, Cl, Fe, K, Mg, P, S, Si, Ti, V, and Zn), and by laser-induced breakdown spectroscopy – LIBS (Ba, Ca, Fe, K, Mg, Na, Si and Ti) both for qualitative purposes. For quantitative analysis of Al, Ba, Ca, Fe, K, Na, S, and Zn, the analytes were extracted from samples of PM10 collected, in filters of glass fibre, with an extractive acid solution (HNO₃ and HCl) and determined by inductively coupled plasma optical emission spectrometry – ICP OES. Spatial variations of elemental concentrations (ng m^–3) were significantly higher in winter Al (19.0), Ba (13.6), Ca (20.0), Na (27.0), S (37.1), and Zn (9.5), autumn showed the highest concentration of Na (26.4), spring showed the highest concentration of Fe (11.6), K (13.1) and also S (25.3) and summer did not show a high concentration in the comparison between the seasons at the site. Using principal component analysis (PCA), as a data interpretation tool, with the data obtained by the WD-XRF and LIBS it was not possible to obtain a good correlation, but with the data of ICP OES, it was possible to verify correlations between identified and determinate elements, with samples collected in the autumn, winter, spring and summer seasons in the city of São Carlos. These associated analytical techniques were excellent tools in environmental monitoring, through the analysis of PM10 samples, presenting reliable and efficiency strategy, and based on the PCA and the EF equation was possible to draw the profile of the possible origins of these elements in the city.     

基于应用型人才培养的分析化学实验教学改革

为适应应用型人才培养的需要,各高校对分析化学实验教学进行了诸多改革。针对我校具体情况,在参照兄弟院校改革方案的基础上,在改革教学内容、优化教学方法、完善考核方式、提升教师素质等方面提出了自己的改革方案,取得了一定的效果。对其他高校进行分析化学实验教学改革具有一定的参考价值。     

A Smartphone-assisted Paper-based Analytical Device for Fluorescence Assay of Hg2+

Rapid, efficiency and portable detection systems in low-resource settings with limited laboratory equipment and technical expertise are urgently needed. Herein, an integrated platform composed of a paper-based analytical device and a smartphone detection system for Hg²⁺ onsite testing was developed. Nitrogen-doped carbon dots(N-CDs) were synthesized by a simple hydrothermal method using citric acid as the carbon source and ethanediamine as the nitrogen source, which gave out bright blue fluorescence under the excitation at 350 nm UV light and the absolute fluorescence quantum yield was 17.1%. The fluorescence of the prepared N-CDs can be effectively quenched by Hg²⁺. In addition, an external attachment of smartphone for illumination and external light interference was designed to trace the fluorescence signals, and a software application of Android system with simple operation program was developed to perform snapshot and image processing. The smartphone-assisted detection system was combined with the N-CDs decorated paper chip to achieve the sensitive detection of Hg²⁺ in water samples. This integrated method for reliable sensing of Hg²⁺ shows a good linear detection range of 10-800 μmol/L(R²=0.9595) with detection limit of 1.07×10^-8 mol/L.     

MOTION OF THE DAMPED FOUCAULT PENDULUM

本文建立运动方程,得到了阻尼傅科摆的解析解,并对解的性质进行了一些探究.文中建立的模型是在理想单摆的基础上增加了科里奥利力和与速度成正比的阻尼力.     

非线性弹性大变形材料梁的抗爆特性

从理论上探讨了非线性弹性大变形材料应用于抗爆结构的可行性,为此,基于等效结构体系的分析原理,将两端固定铰支梁的横向和纵向位移表示为三角级数形式,应用第二类Lagrange方程建立了非线性大变形材料梁的非线性分析方法,并且用ABAQUS有限元软件中的超弹性材料模型验证了所提出的方法的有效性。对典型的爆炸荷载作用下非线性弹性大变形材料梁的抗爆特性进行了分析,讨论了动力放大系数和材料性质及动荷载之间的关系。结果表明:与线弹性小变形材料相比,非线性弹性大变形材料具有优良的抗爆特性,结构的抗爆能力随结构变形的增大而显著提高。     

热应力作用下层合路面结构层间剥离内力分析

温度变化会造成环氧树脂-混凝土特种路面结构边缘处产生脱层现象.基于层合结构热应力的两变量解析模型,推导了层间剥离力矩和剥离剪力.该方法避免了采用弹性理论求解局部复杂应力状态的困难,为解决层合结构边界脱层问题提供了新途径;由此,研究了环氧树脂层的厚度和弹性模量对层间剥离内力和混凝土层最大拉应力的影响.计算结果表明,通过控制环氧树脂层厚度和弹性模量,可有效降低剥离内力,避免剥离破坏.     

Fibre-reinforced composites with fibre-bending stiffness under azimuthal shear – Comparison of simulation results with analytical solutions

In Ref. [1], Spencer and Soldatos proposed an enhanced modelling approach for fibre-reinforced composites which accounts for the fibre-bending stiffness in addition to the directional dependency induced by the fibres. Although analytical solutions for simple geometries have been derived over the past years, often subject to specific assumptions such as small deformation kinematics, the application to more general and non-academic boundary value problems is desirable. Motivated by the latter, the numerical solution of the general system of partial differential equations by means of a multi-field finite element approach is proposed in Ref. [2] and the principal model properties are studied for a specific form of the elastic energy potential. In the present contribution a comparison of the numerical solution by means of the multi-field finite element approach against the analytical solution is presented for the azimuthal shear deformation of a tube-like structure. To this end, the general deformation pattern and especially the distribution of the stress and couple stress tensor are taken into account. We find that, although the analytical solution is derived subject to the assumption of small deformations, whereas the numerical solution is based on the finite strain counterpart of the theory, the simulation results are quasi identical, which verifies the numerical framework proposed.     

Scattering States of l-Wave Schrödinger Equation with Modified Rosen-Morse Potential

Within a Pekeris-type approximation to the centrifugal term, we examine the approximately analytical scattering state solutions of the l-wave Schrödinger equation with the modified Rosen-Morse potential. The calculation formula of phase shifts is derived, and the corresponding bound state energy levels are also obtained from the poles of the scattering amplitude.