人钠/碘转运体基因转染结肠癌SW480细胞及相关蛋白表达的检测

目的以重组人钠/碘转运体（hNIS）基因转染结肠癌SW480细胞并检测hNIS mRNA及蛋白的表达，为放射性碘治疗非甲状腺肿瘤提供新思路。方法将构建好的重组质粒（pcDNA3.1+-hNIS）进行酶切、测序鉴定并扩增、提取。SW480细胞分为重组质粒（pcDNA3.1+-hNIS）转染组、空白质粒（pcDNA3.1+）转染组、空白对照组，转染后以RT-PCR和Western blot检测各组细胞hNIS mRNA和蛋白的表达。结果酶切和测序结果显示插入的hNIS基因大小和方向均正确。RT-PCR和Western blot显示重组质粒转染组SW480细胞可见hNIS mRNA和蛋白的表达，而空白对照组和空白质粒转染组均未检测到hNIS mRNA和蛋白的表达。结论脂质体法可有效地将hNIS基因转染至SW480细胞并成功表达hNIS蛋白。     

Silica iodide catalyzed,ultrasound-promoted,one-pot four-component synthesis of novel 1,4,5,6-tetrahydropyridine-3-carboxylate derivatives

Silica iodide catalyzed, competent synthesis of 15 novel substituted-1,4,5,6-tetrahydropyridine-3-carboxylate derivatives was achieved by a one-pot four-component cyclocondensation of Meldrum's acid, aryl aldehydes, aromatic amines and ethyl acetoacetate under ultrasonic irradiation. In comparison to the conventional methods, our protocol is convenient, eco-friendly and offers several benefits such as: mild conditions, high yields, shorter reaction time and ease in product isolation.     

trans-Hydroboration of Propargyl Alcohol Derivatives and Related Substrates

The hydroboration of internal alkynes with pinacolborane as the reagent catalyzed by [Cp*RuCl]₄ results in good to excellent levels of regio- as well as stereoselectivity, provided that the triple bond bears one linear and one singly-branched substituent. In such cases, the reaction follows an unusual trans-addition mode and places the boron entity distal to the branching point. The resulting alkenyl boronates, which are difficult to make otherwise, can be engaged in numerous enabling downstream processes.     

Highly Selective Asymmetric Intramolecular Selenocyclisation

Asymmetric intramolecular selenocyclisation of alkenoic acids, alkenols and alkenyl urethanes using chiral ferrocenylselenenyl cations proceeds smoothly to give the corresponding lactones, cyclic ethers and N-heterocyclic compounds, respectively, in moderate yields with very high diastereoselectivities.     

Third Order Derivative Spectrophotometry Determination of Mercury (II) as the Ternary Complex with Iodide and Pyronine G

Abstract

This paper describes a third order derivative mole cular absorption spectrophotometric procedure for the deter mination of traces of mercury based on the formation of ternary ion-association complex by reaction of mercury with pyronine G in the presence of iodide. The pink coloured ternary ion-association complex is readily formed in the pH range 1–7 and remained stable for 24 h. after stabilization with gelatin. The influence of various instru mental parameters and reaction conditions for maximum color development are discussed. The few interfering ions can be masked by the addition of appropriate reagent solutions.     

Synthesis of Homoallylic Alcohols From Ketones in Water

Homoallylic alcohols have been prepared in good yields by allylation of ketones with allyl bromide in the presence of stannous chloride dihydrate, zinc iodide, and ammonium chloride in water.     

Copper (I) Iodide-Catalyzed Solvent-Free Synthesis of α-Aminophosphonates

Abstract

A method for the synthesis of α-aminophosphonates through the three-component coupling reaction of aldehydes, amines, and diisopropyl phosphite using copper (I) iodide salt catalyst is demonstrated, The reaction is highly efficient, economic, and also environment friendly.

[Supplemental materials are available for this article. Go to the publisher's online edition of Phosphorus, Sulfur, and Silicon and the Related Elements for the following free supplemental resource: Table S1, Figures S1–S9.]

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