敞开式质谱成像方法的组织样品稳定性研究

以添加镇静催眠候选新药N6-羟苄腺嘌呤核苷[N6-(4-hydroxybenzyl)adenine riboside,NHBA]的组织匀浆切片作为考察对象,对空气动力辅助离子化质谱成像系统(Air Flow Assisted Ionization Mass Spectrometry Imaging,AFAI-MSI)的关键参数进行考察与优化,以保证最佳条件进行样品检测。在此基础上,对预处理的整体大鼠组织切片进行平行的连续两次质谱成像分析,考察了其内源性代谢物在成像分析过程中是否发生变化。通过对采集数据中关键质谱峰的筛选等处理步骤,并采用可视化的主成分分析(PCA)方法,开展了组织样品内源性代谢物的稳定性分析,最终验证了采用该样品前处理和质谱成像方法,能够保证组织切片样品中内源性代谢物的稳定性,为质谱成像分析结果提供了可靠依据。     

An Experimental Study of Liquid Jets Interacting with Cross Airflows

The primary break‐up of liquid jets in cross flows has been studied experimentally. An open‐circuit wind tunnel was employed in which the airflow was generated by a centrifugal fan. The test section, positioned 3 m downstream of the fan, was made of clear acrylic resin to allow optical access and visualization. The working liquid used in the present experiment was an aero‐engine lubrication oil, which was injected perpendicularly into the air flow, via a nozzle placed in the top wall of the test‐section. The study of the primary break‐up mechanisms of the jet involved three parameters, the oil viscosity, and the jet and air cross flow velocities, which were varied independently. Two different break‐up regimes were observed and identified; arcade break‐up and bag break‐up. These were separated by a transition zone. Transverse and longitudinal (or streamwise) penetrations of the jet before the liquid breakup were also measured. The correlation proposed by Wu et al. to predict the jet transverse penetration before the break‐up of the liquid, as a function of the liquid/airflow momentum‐flux ratio, was found to be applicable only to liquids with low viscosity. An empirical extension to this equation has been produced.     

孔板空调风口送风射流的数值模拟

介绍Ｎ点风口模型用于数值模拟室内空气流动时描述孔板类送风口的入流边界条件．然后采用该风口模型对不同的孔板风口出流条件算例进行数值计算，并就轴心速度衰减、射流扩展角以及断面流速分布等射流特性与实验数据进行了对比．比较结果表明，Ｎ点风口模型用于描述数值模拟室内空气流动的孔板类风口入流边界条件，可以获得工程上足够满意的结果．     

Influence of Porous Media and Airflow Rate on the Fate of NAPLs Under Air Sparging

Nonequilibrium air–water mass transfer experiments using a laboratoryscale singleair channel setup were conducted to investigate the influence of porous media and air velocity on the fate of nonaqueous phase liquids (NAPLs) under air sparging conditions. Benzene was used as a NAPL while silica sand 30/50 (dp₅₀=0.305mm, uniformity coefficient, UC=1.41) and silica sand 70/100 (dp₅₀=0.168mm, UC=1.64) were used as porous media. Air velocities ranged from 0 to 1.4cm/s. Mass transfer coefficients for the dissolution of NAPLs were estimated by numerical methods using a twodimensional dissolution–diffusion–volatilization model. The study showed that the presence of advective airflow in air channels controlled the spreading of the dissolved phase but the overall removal efficiency was independent of airflow rate. Removal efficiencies and dissolution rates of the NAPL were found to be strongly affected by the mean particle size of the porous media during air sparging. More than 50% reduction in the removal rate of benzene was found when silica sand 70/100 was used instead of silica sand 30/50. Mass transfer coefficients for the dissolution of benzene NAPL were estimated to be 0.0041cm/min for silica sand 70/100 and 0.227cm/min for silica sand 30/50. Increasing the air velocity from 0.6 to 1.4cm/s for silica sand 30/50 did not result in a higher removal rate. Quantitative estimation of the dissolution rates of benzene NAPL indicated that the dissolution rates (between 0.227 and 0.265cm/min) were similar in magnitude for the same porous media but different air flow rates. Based on the visualization study, air sparging may be used to control the spreading of the dissolved phase even when the glob of NAPL is several centimeters away from the air–water interface of the air channels.     

Analysis of hysteresis and phase shifting phenomena in unsteady three-dimensional wakes

The aerodynamic characteristics of automobiles are greatly influenced by the unsteady change in the direction of relative airflow. The aim of this paper is to analyse how such a change influences vehicle wake flow patterns. An analysis was conducted on a simplified model capable of reproducing the typical structures encountered under the aerodynamic conditions of an automobile. The results were processed by mapping the steady and unsteady total pressure losses around the model. The findings should enable automobile development engineers inter alia to identify and analyse the physical phenomena that occur when a vehicle is subjected to a sudden gust of side wind.List of symbols B rod - l length of rod B (m) - angle of rod B (degrees) - D disk - P connection point between disk D and rod B - M _o drive motor of disk D - O centre of rotation of disk D - e radius of disk D (m) - angle of disk (degrees) - t moment of time t (s) - _o angle (degrees) at instant of time t=0 - d diameter of model (m) - C centre of rotation of model - x _c abscissa of centre of rotation C of model (m) - M connection point between model and rod B - pi value=3.14159 - incidence of model (degrees) - _M maximum value of incidence (degrees) - _m minimum value of incidence (degrees) - angular amplitude (degrees) - _c critical angle of incidence (degrees) for steady evolutions - critical angle of incidence (degrees) for the unsteady evolutions - ̄ mean angle of incidence (degrees) - angle (degrees) of the model such that =+ - pulse (rad s^–1) - T period (s) - f frequency (Hz) - R radius of model in meter (m) - velocity vector of incident airflow - V _o intensity of velocity vector (m s^–1) - P _io total pressure associated with upstream airflow velocity (Pa) - P _i local total pressure (Pa or J m^–3) - density (kg m^–3) - C _x drag coefficient - total pressure coefficient - (m, n) dimensions of grid: lines m, columns n - x X coordinate of sampling plane (m) - y _j Y coordinate of point of index j for j[1,n] - z _k Z coordinate of point of index k for k[1,m] - P _i (x,y _j ,z _k ,(t)) continuous data of unsteady total pressure (Pa) - discrete data of unsteady total pressure (Pa) - N number of tomographic images, from 1 upwards over an oscillation period T - maximum value of total pressure coefficients for steady evolutions - maximum value of total pressure coefficients for unsteady evolutions and increasing incidences - maximum value of total pressure coefficients for unsteady evolutions and decreasing incidences - differential between and - differential between and - phase shifting (degrees)     

Glottal closure, transglottal airflow, and voice quality in healthy middle-aged women

Seventeen healthy women, 45 to 61 years old, were examined using videofiberstroboscopy during phonation at three loudness levels. Two phoniatricians evaluated glottal closure using category and ratio scales. Transglottal airflow was studied by inverse filtering of the oral airflow signal recorded in a flow mask (Glottal Enterprises System) during the spoken phrase /ba:pa:pa:pa:p/ at three loudness levels. Subglottal pressure was estimated from the intraoral pressure during p occlusion. Running speech and the repeated /pa:/ syllables were perceptually evaluated by three speech pathologists regarding breathiness, hypo-, and hyperfunction, using continuous scales. Incomplete glottal closure was found in 35 of 46 phonations (76%). The degree of glottal closure increased significantly with raised loudness. Half of the women closed the glottis completely during loud phonation. Posterior glottal chink (PGC) was the most common gap configuration and was found in 28 of 46 phonations (61%). One third of the PGCs were in the cartilaginous glottis (PGCc) only. Two thirds extended into the membranous portion (PGCm); most of these occurred during soft phonation. Peak flow, peak-to-peak (AC) flow, and the maximum rate of change for the flow in the closing phase increased significantly with raised loudness. Minimum flow decreased significantly from normal to loud voice. Breathiness decreased with increased loudness. The results suggest that the incomplete closure patterns PGCc and PGCm during soft phonation ought primarily to be regarded as normal for Swedish women in this age group.     

气流动态特征管内迁移研究

空调环境的动态化是对传统空调的改进,而送风动态化是实现空调环境动态化的一个途径。气流紊动特性是影响建筑热环境和人体舒适性的重要因素之一。利用FLUENT软件对速度入口呈模拟自然风波动的气流在管道中的流动进行了模拟,选择k-ε模型求解,分析其动态特征的变化。分别为:湍流强度、速度概率分布偏斜度、频谱分布。计算结果表明了几种典型的送风管道管件出口处湍流强度、风速概率分布偏斜度、功率谱密度曲线的变化情况;人体敏感区域(0.01—1Hz)区间双对数功率谱密度曲线的负斜率增大。这种方法为管道入口的送风提供了参考依据。     

地铁吸声材料的吸声性能和流阻测定

制备了以陶粒为骨料的多孔状地铁吸声材料.研究不同的陶粒粒径及级配,水灰比,水泥掺量以及掺和料对材料吸声性能和流阻的影响.结果显示:提高小陶粒的掺量,减小水灰比,减少水泥用量以及掺入一定的掺和料(膨胀珍珠岩,硅灰)对提高材料的吸声性能是有利的.材料的流阻不是越大越好,获得最佳吸声性能和强度的材料时,有一个最佳流阻值,为1,475 Pa·S/m3.该样品的组成为25;水泥(质量分数,下同),75;大尺寸(2.4～4.8 mm)陶粒,外加4.2 g减水剂,水灰质量比为0.3,外掺膨胀珍珠岩占骨料的5;,硅灰占水泥的12;.     

冷库预冷流动传热物理场分布研究

冷库预冷是传统的果蔬采后预冷方法,前人研究通过数值模拟使冷库预冷过程的流场和温度场可视化,本文进一步分析空气流速、局部平均空气龄、温度、熵产、?损和(煨)耗散等物理场的分布特性,指出其间存在密切联系.塑料筐周围的空气流速和流向与内部的局部平均空气龄有关,并影响内部的传热速率.传热熵产率、传热?损率和(煨)耗散率的分布特...     

受对流边界层影响的过山气流

建立了一个自由大气与对流边界层相耦合的解析模式，利用该模式系统地讨论了对流边界层对过山气流的影响。