6—磺酰氯香豆素用作发光标记物的初探

过量C6SCl与丙氨酸在pH 9.11(H_3BO_3-NaOH)缓冲体系中,40℃反应40min,经乙酸乙酯萃取分离后,点样在层析聚酰胺膜上,用甲酸+水(1+1)展开,衍生产物得到很好的分离。斑点经1 mol·L~(-1)NaOH喷雾,紫外灯预光照10 min和干燥后,荧光强度与在4～60×10~(-11)mol范围内的丙氨酸量呈良好线性关系。在滤纸基质上,以Pb(AC)_2作重原子微扰剂,标记产物还能产生较强的室温磷光发射。研究表明,C6SCl不仅能作为氨基酸等的荧光标记物,而且可望作为其室温磷光的标记物。     

光度检测离子色谱法测定大豆中的植酸

本文介绍了用光度检测离子色谱法分离分析植酸的适宜条件。以NaNO_3和HNO_3为淋洗液,用磺基水杨酸和三氯化铁为柱后衍生试剂,使用国产YSA型阴离子分离柱分离和测定了大豆中的植酸,得到满意的结果。     

Chemiluminescence Determination of Benzoic Acid Using A Solid-Phase Verdigris Reactor

Benzoicacidisacommonadditiveusedwidelyasfoodpreservativeandplasticizer.Itcouldpromoteseverereactiontoallergicpopulationevenatlowconcentrationlevel1.Routinemethodsfortheassayofbenzoicacidincludeschromatographic2-6,spectrophotometry7,8,capillaryelectrophoresis9-13andelectrochemicaltitration14.Stokes15etal.monitoredairbornebenzoicacidbasedonsurface-enhancedRamanscatteringtechniques.Thereisalsoreportonamicrobialsensorusingpseudomonasforbenzoicacidandtheirderivativesinaqua16.Interestofusinglumines…     

Direct melting polycondensation and characterization of poly(ɛ-caprolactone-co-lactic acid)

A kind of biodegradable material, poly(ɛ-caprolactone-co-lactic acid) [P(CL-co-LA)] was synthesized via the direct melting polycondensation of lactic acid (LA) and ɛ-caprolactone (CL). The influences of the polycondensation time, and the catalyst type on the intrinsic viscosity of P(CL-co-LA) were also investigated. The results indicate that P(CL-co-dl-LA), with an intrinsic viscosity of 0.4733 dL/g, can be prepared by direct melting polycondensation with the molar ratio LA/CL = 3:7 at 180°C and 70 Pa for 12 h, using 0.5% (mass fraction) of SnCl₂ as the catalyst. Compared with lactide ring-opening polycondensation (ROP), the direct melting polycondensation of LA and CL is more practicable and simple. Translated from Journal of South China University of Technology, 2006, 34(7): 7–11 (in Chinese)     

二磺酸掺杂高热稳定性导电聚苯胺的合成及性能

以有机二磺酸作为掺杂剂合成了具有高热稳定性的二磺酸掺杂导电聚苯胺。研究了反应时间、温度、酸/苯胺摩尔比等因素对产率、产物的导电率与分子量的影响。利用微波加热的方法测试有机二磺酸掺杂聚苯胺的热稳定性能，结果表明：有机二磺酸掺杂的导电聚苯胺在微波场中升温速率快，并且具有良好的反复升温性能。     

Analysis of the disaccharides derived from hyaluronic acid and chondroitin sulfate by capillary electrophoresis with sample stacking

CE conditions for monitoring the unsaturated disaccharides of hyaluronic acid (di-HA) and chondroitin sulfate (di-CS) using an alkaline tetraborate buffer, electrokinetic sample injection, and UV absorption detection at 232 nm are reported. Separations were performed in an uncoated fused-silica capillary having reversed polarity and reversed electroosmosis generated with the addition of CTAB to the buffer. The influence of various separation parameters, including the concentration of CTAB, buffer pH, concentration of tetraborate, and applied voltage, on the resolution of the two disaccharides was investigated. Baseline separation was obtained with 25 mM tetraborate at pH 10.0 and having 0.05 mM CTAB. Chloride and phosphate in the sample are beneficial for the stacking of the disaccharides, with di-HA forming a much sharper peak than di-CS. Using samples prepared in 25 mM Tris-HCl (pH 7.5) and electrokinetic injection at the cathode at -10 kV for 40 s, linear relationships between the corrected peak area and the concentration of the disaccharides have been found in the ranges of 1.0-400.0 and 0.1-1.0 microg/mL (0.2-1.0 microg/mL for di-CS), with correlation coefficients being >0.9933 in all cases. The RSDs of detection times and corrected peak areas were between 1.13-1.24 and 1.57-2.13%, respectively. Applied to human serum samples that were prepared by ethanol precipitation and depolymerization of the two polysaccharides with chondroitinase ABC reveals comigration of endogenous compounds with di-HA and a sample-dependent detection time. The di-HA content in the serum sample can be estimated via subtraction of the blank peak that is obtained without enzymatic hydrolysis.     

Selective Oxidation of Isobutene over CsFeCoBiMnMoOx Mixed Oxide Catalyst

Mixed oxide catalyst Cs0.1Fe2Co6BiMnMo12Ox was prepared by the coprecipitation method.Selective oxidation of isobutene was carried out in a fixed-bed reactor over Cs0.1Fe2Co6BiMnMo12Ox.The results showed that the catalyst had high catalytic activity. Under the optimum reaction conditions(n(i-C4):n(O2)=1:2-1:4, space velocity=180 h^-1, T=360℃), the yields of methacrolein and methacrylic acid can reach 80% and 8%, respectively. The total yield of liquid products (methacrolein, methacrylic acid and acetic acid) can reach about 90%.     

有机锡配合物[(o-Cl-PhCH2)2Sn(OCOC9H6N)2]·2(C6H6)的合成和晶体结构研究

0引言某些有机锡羧酸酯化合物具有杀虫、杀菌和抗癌活性,引起人们极大的兴趣[1~3]。近年来的研究结果表明,该类化合物中心锡原子的配位形式既决定于与锡原子直接相连的烃基的结构,也与配体的类型有关,尤其是含有孤电子对的杂环酸的引入,导致了许多新型结构有机锡化合物的产生[4     

树脂吸附法处理水杨酸甲酯生产废水的研究

采用吸附树脂NDA—99处理水杨酸甲酯生产废水，结果表明该树脂时废水中的5磺基水杨酸及水杨酸均具有良好的吸附—脱附性能．废水经预处理和吸附处理后，CODCr由57000-59000mg／L降至6300mg／L左右，去除率接近89％．用IBV8％NaOH 3BVH2O作脱附剂，在温度为60℃、流量为IBV／h的条件下，脱附率接近100％，树脂可重复使用．高浓度脱附液经酸化、浓缩、冷却结晶，可回收5—磺基水杨酸，回收率为95％左右。纯度为78％。     

纳米稀土谷氨酸咪唑三元配合物的合成、表征及抗菌活性研究

合成了6种新型纳米稀土三元配合物。通过元素分析、摩尔电导、红外光谱、紫外光谱、氢谱、碳谱、热重-差热等表征,确定了该类配合物的化学组成为：RE（Glu）3ImCl3·3H2O（RE=La^3＋,Nd^3＋,Er^3＋,Eu^3＋,Y^3＋,Gd^3＋;Glu=L-谷氨酸;Im=咪唑）,TEM电镜测试表明其有一定规则形貌,粒径在30-60 nm。通过抗菌实验对其抑菌效果进行研究,结果表明：6种纳米稀土三元配合物对大肠杆菌、金黄色葡萄球菌和白色念珠菌均有较强的抑制作用（最小抑菌浓度MIC分别约为140,100,250μg·ml^-1）,属于广谱抗菌剂;抗菌效果明显优于非纳米稀土三元配合物、稀土氯化物、L-谷氨酸或咪唑。