Introduction of Fluorometry to the Screening of Protein Crystallization Buffers

A system to use fluorometry for the search of protein crystallization buffers was developed. The screening of candidates was done with a use of short gel-filtration column (Sephacryl S-100 HR) linked to the fluorometer. Protein elution was monitored by intrinsic fluorescence or emission from its labels. This method was applied to actin and actin complexes. When nuclei were formed in actin solution, preceding the peak of actin, a new peak appeared on the elution curve. It was found that the fluorescence allows detection of even small amount of nuclei formed in the buffer. Using the screened buffers, crystal growths were attempted. The images of crystals were captured by fluorescence microscope. The monitoring of nuclei with this method will accelerate the screening of crystallization buffers. This system is applicable to the crystallization of other proteins and their complexes.     

Gottfried Kränzlin,the first follower of Tswett

Summary This paper summarizes the work of Gottfried Kränzlin, who, in 1906–1908, was the first after Tswett to utilize chromatography for the investigation of plant pigments.     

Demonstration of an optimized flow cell SFC-FTIR interface

Summary An on-line application of capillary SFC-FTIR for the analysis of five steroids has been demonstrated. Baseline resolution is achieved for all the components of the mixture and IR spectra are obtained which match well with spectra of the neat materials. Also, the injected minimum detectable quantity (IMDQ) of caffeine (approximately 2 ng) under similar chromatographic conditions is demonstrated for this interface. Very low IMDQ's achieved using this flow cell interface will facilitate the use of this technique for the analysis of biological fluids and other materials where very low detection limits are required.     

Scale-up of “myxalin” purification by a pseudoaffinity method using a radial flow column

Summary Myxalin is a newly defined bacterial anticoagulant. It has been previously purified on Histidyl-Sepharose, on a small scale. We describe a scale-up of this procedure using a radial flow column (Sepragen^?). This design permits the use of the same soft gel matrix with 15-fold increase in flow rate compared to the linear system and a simple extrapolation of the operating conditions when optimized on a smaller scale gave no decrease in resolution. There are moreover, better activity yields and very interesting economic advantages.     

Comparative study of some commercial chemically bonded phases in classical reversed-phase chromatography and in ion-pair reversed-phase liquid chromatography

Summary The behaviour of various octadecyl commercial bonded phases are compared in classical reversed-phase chromatography and in ion-pair reversed-phase chromatography. Great differences are exhibited by the packings studied according to the polarity of the solutes. Whereas hydrocarbonaceous bonded phases show very similar selectivity versus apolar or weakly polar solutes, great differences are observed when analyzing more polar solutes even when ion-pair reversed-phase chromatography is performed.Presented at the 14th International Symposium on Chromatography London, September, 1982     

Resolution of Ibuprofen Catalyzed with Free and Immobilized Lipases

Enzymatic hydrolyses of ibuprofen esters produced 99% optically pure S-(+)-ibuprofen. The ee values of two enantiomers of ibuprofen were determined on a HPLC equipped with a chiral column.     

计算精馏塔理论塔板数和加料板位置的新方法（Ⅱ）

通过作平衡曲线的弦，导出了计算精馏塔理论塔板数的显函数关联式，并确定了式中常数Ｃ、Ｅ的值．该式直接将理论塔板数和操作参数关联，在参数变化较大的范围内，其计算结果与Ｓｍｏｋｅｒ方程的精确解一致．     

Regular simplices and lower volume bounds for hyperbolicn-manifolds

For an-dimensional compact hyperbolic manifoldM ⁿ a new lower volume bound is presented. The estimate depends on the volume of a hyperbolic regularn-simplex of edge length equal to twice the in-radius ofM ⁿ. Its proof relies upon local density bounds for hyperbolic sphere packings.