4,5-二溴苯基荧光酮-钛(Ⅳ)-Tween-80分光光度法测定尿蛋白

本文提出了一个借钛-4,5-二溴苯基荧光酮(DBPF)-Tween-80与蛋白质显色反应光度测定微量尿蛋白的高灵敏方法。在pH2.9,血清蛋白能与Ti-DBPF-Tween-80形成蓝色络合物,最大吸收波长为605nm。加入小量乙醇能提高方法的灵敏度和稳定性,牛血清蛋白含量在0～150μg/25ml范围内服从Beer定律。尿液中其它成分不干扰测定。用拟定的方法测定人尿蛋白,获得满意结果。     

钒(Ⅴ)-硫氰酸盐-碱性三芳基甲烷染料的显色反应研究

研究了在表面活性剂存在下,钒(V)与硫氰酸盐和结晶紫、孔雀绿、灿烂绿、甲基紫及维多利亚蓝4R等碱性三芳基甲烷染料形成离子缔合物的显色反应.讨论了钒(V)-硫氰酸盐-结晶紫-阿拉伯树胶体系的反应条件和分析特性。摩尔吸光系数为2.55×10⁵L·mol^-1·cm^-1.此法可用于铝合金中微量钒的测定。     

meso－四（4－甲基－3－磺基苯）卟啉分光光度法测定痕量钯的研究

本文研究了Ｐｄ（Ⅱ）与ｍｅｓｏ－四（４－甲基－３－磺基苯）卟啉（Ｔ（４Ｍ３ＳＰ）Ｐ）的显色反应在表面活性剂十二烷基磺酸钠（ＳＤＳ）和抗坏血酸存在下，ｐＨ４８的ＨＡｃ－ＮａＡｃ介质中，沸水浴加热，Ｐｄ（Ⅱ）与Ｔ（４Ｍ３ＳＰ）Ｐ形成１∶１（Ｍ∶Ｌ）配合物配合物最大吸收波长４１４ｎｍ，表观摩尔吸光系数ε＝２０×１０５Ｌ·ｍｏｌ－１·ｃｍ－１钯量在０－２．２５μｇ／１０μＬ范围内符合比耳定律本法应用于催化剂中痕量钯的测定，结果满意     

合成的近红外花菁染料测定痕量血清蛋白质

染料结合法测定蛋白质在生化及临床分析中已有广泛应用,常用的染料有溴甲酚绿、溴酚蓝、考马斯亮蓝及铬天青S等,这些染料的最大吸收波长均处于可见光区.近年来,近红外染料越来越引起人们的注意.     

利用紫外光离子源高场不对称波形离子迁移谱快速、现场检测水中氨的含量

氨是水体主要的污染物之一,其含量是水质评估的重要参数。本研究采用真空紫外光离子源-高场不对称波形离子迁移谱(Ultraviolet photoionization high field asymmetric waveform ion mobility spectrometry, UVFAIMS)技术,发展一种的水中氨含量的现场快速检测方法。通过对比标准氨样品和水中微量氨UV-FAIMS 谱图峰的特征补偿电压(Compensation voltage, CV)值,确定了水中HN+4的特征离子峰位置;研究了不同分离电压(Dispersion voltages, DV)下HN+4谱图峰位置的关系,获得了HN+4的特征识别系数α2和α4分别为2.21×10-5 Td-2和-1.45323×10-9 Td-4;通过不同浓度样品的信号响应,研究了UV-FAIMS 对水中氨的检出限,在信噪比为3的情况下达到了9.2 μg/ L。本研究为水中氨现场检测提供了一种快速、无需前处理的技术手段。     

Hemicyanine-linked pyrimidine mimics as solvatochromic fluorophores with visible excitation wavelengths

The design of solvatochromic fluorescent nucleosides with visible excitation wavelengths is a goal towards the generation of modified oligonucleotides for fluorimetry-based molecular imaging. Herein, two hemicyanine-linked C5-2′-deoxyuridine nucleosides (PyI-dU and APPy-dU) have been synthesized by first generating hemicyanine-alkyne precursors that were attached via the alkyne moiety to 5-iodo-2′-deoxyuridine (5-I-dU) by Sonogashira coupling. The photophysical properties of the hemicyanine-linked dU probes have been characterized and compared to the corresponding properties of the hemicyanine-alkyne precursors. The nucleoside probe PyI-dU exhibits optical features that mimic the properties of the free hemicyanine-alkyne precursor, while the APPy-dU probe displays more favorable optical properties (longer excitation wavelength, brighter emission in water) than its precursor that is ascribed to π-stacking interactions between the hemicyanine dye with the dU nucleobase. Overall, probe APPy-dU is a superior solvatochromic fluorophore than PyI-dU suggesting its greater utility for fluorescent imaging applications.     

卡尔曼滤波分光光度法同时测定水中微量砷和磷

研究了砷钼酸和磷钼酸与乙基罗丹明Ｂ的缔合物的吸收光谱，并以卡尔曼滤波算法对这两个严重重叠的光谱进行数据解析，达到了不需分离和掩蔽而同时测定砷和磷的目的，本法准确，精密，应用于水样分析，获得满意结果。     

小波变换-主成分回归分光光度法同时测定邻苯二酚、间苯二酚和对苯二酚三组分

根据小波变换具有将信号分频的特点,本文提出了将小波变换与主成分回归(PCR)相结合的一种多元校正算法。该法能更有效地去除噪声,提取有用信息,并将其用于分析邻苯二酚、间苯二酚、对苯二酚三组分体系。实验结果表明,本法比直接用主成分回归处理效果好,得到的平均相对误差从2．24％降低到1．19％。     

Development of novel detection reagent for simple and sensitive determination of trace amounts of formaldehyde and its application to flow injection spectrophotometric analysis

In this paper, a novel detection reagent for formaldehyde determination is proposed, and is applied to a simple and highly sensitive flow injection method for the spectrophotometric determination of formaldehyde. The method is based on the reaction of formaldehyde with methyl acetoacetate in the presence of ammonia. The increase in the absorbance of the reaction product was measured at 375 nm. An inexpensive light emitting diode (LED)-based UV detector (375 nm) was, for the first time, used. Under the optimized experimental conditions, formaldehyde in an aqueous solution was determined over the concentration range from 0.25 to 20.0 × 10⁻⁶ M with a liner calibration graph; the limit of detection (LOD) of 5 × 10⁻⁸ M (1.5 μg L⁻¹) was possible. The relative standard deviation of 12 replicate measurements of 5 × 10⁻⁶ M formaldehyde was 1.2%. Maximum sampling throughput was about 21 samples/h. The effect of potential interferences such as metals, organic compounds and other aldehyde was also examined. The analytical performance for formaldehyde determination was compared with those obtained by the conventional acetylacetone method, which uses visible absorption spectrophotometry. Finally, the proposed method was successfully applied to the determination of formaldehyde in natural water samples.     

顺序注射停流分光光度法测定痕量碘

食物中碘的含量一直是人们关注的问题,研究食物中微量碘的分析方法具有重要的现实意义.目前测定碘的方法主要有色谱法[1,2]、荧光法[3,4]、分光光度法[5,6]等.