Nd-四甘醇醛缩苯丙氨酸Schiff碱配合物与DNA相互作用的光谱研究

研究了Nd-四甘醇醛缩苯丙氨酸Schiff碱配合物(NdL)与天然和变性DNA的相互作用.在DNA存在下,测定了NdL的紫外吸收光谱(UV)和荧光激发光谱(FL).利用紫外滴定法,测定了NdL与DNA的结合常数.在20mmol/L Tris-HCl缓冲溶液(pH=7.0),Nd-四甘醇醛缩苯丙氨酸Schiff碱配合物与小牛胸腺DNA的结合常数为4.11×103L/mol,化合物本身的UV和FL峰强度减小.NdL与DNA的作用模式为"静电引力".     

清开灵注射液生产过程中金银花提取液的在线质量控制方法研究

为建立清开灵注射液生产过程中的中间体金银花提取液快速准确的在线质量控制方法, 通过对金银花提取液的UV原始光谱、一阶导数光谱进行波长点的数据筛选,选择与HPLC法测得的绿原酸含量相关性最好的光谱类型和波长点进行建模,建立绿原酸含量的UV预测方程。另取10批金银花提取液检验预测结果的可靠性。UV原始光谱中最佳波长为294 nm(r=0.991 9,n=28);一阶导数光谱中最佳波长为316 nm(r=0.995 9,n=28)。绿原酸含量的预测方程为：c(mg·mL-1)＝506.254 3×A_{316 nm}＋0.177 1。经检验,预测方程准确、可靠(r=0.997 1,n=10),可直接应用于在线检测。绿原酸含量的预测结果为：约90％的金银花提取液中绿原酸含量分布为0.4～4.0 mg·mL-1。此法简单、快速、准确,可作为清开灵注射液生产过程中金银花提取液的在线质量控制方法。     

Fluorescence and absorption of polystyrene exposed to UV laser radiation

We show that when polystyrene is exposed (for 15–60 sec) to a UV laser light beam (λ = 248 nm), its absorption and luminescent properties change significantly. In the irradiated polymer, optical centers are formed with absorption bands in the 280–460 nm region and fluorescence bands in the 330–520 nm region. We have established the chemical structure of the optical centers for fluorescence of polystyrene. __________ Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 1, pp. 54–58, January–February, 2006.     

Effect of IR laser radiation on the structure and optical properties of PC-PBT/Pd polymer nanocomposite films

ABSTRACT

Bayfol (PC-PBT blend ?lm) is a class of polymeric solid-state nuclear track detector which has a lot of applications in several radiation detection ?elds. It is a bisphenol-A polycarbonate PC blended with polybutylene terephthalate PBT. Bayfol/Palladium (PC-PBT/Pd) nanocomposite films have been deposited using the molding technique. It is worth mentioning that this report is almost the first one dealing with the topic of the changes of physical properties of Bayfol/Pd nanocomposite due to laser exposure. Samples from PC-PBT/Pd (5?wt%) nanocomposite were exposed to IR-pulsed laser of 5-W power, capable of producing 2000 pulses per second with pulse duration of 200?ns at 904?nm. The laser fluences were in the range 2–25?J/cm². The resultant modi?cations in the exposed nanocomposite samples have been studied as a function of fluence using different characterization techniques such as X-ray diffraction (XRD), UV spectroscopy and color difference studies. The results indicate the proper dispersion of Pd nanoparticles in the PC-PBT matrix that causes a strong intermolecular interaction between Pd and PC-PBT, resulted in an increase in refractive index and the amorphous phase. Also, it is found that the laser exposure reduces the optical energy gap that could be attributed to the increase in structural disorder of the exposed PC-PBT/Pd nanocomposites due to crosslinking. Further, the color intensity ΔE, which is the color difference between the exposed samples and the non-exposed one, was increased with increasing the laser fluence, convoyed by a significant increase in the green and yellow color components.     

新型金阴极及其紫外光电发射特性

采用直流磁控溅射法制备了不同厚度的金纳米薄膜,在高纯氮气气氛、800 ℃条件下快速退火,在石英基底上制备了具有表面微纳颗粒的新型金阴极。应用扫描电子显微镜对阴极的表面形貌进行表征,结果表明：阴极表面形成了均匀分布的金纳米颗粒,平均粒径随金纳米薄膜厚度的增加（5 nm至20 nm）从300 nm增大到800 nm。在190~360 nm紫外光下,对阴极的光电子发射特性进行了研究,结果表明：相对于平面阴极,新型金阴极的光电子发射效率提高了10倍以上,最高可达到平面阴极的16倍,且随颗粒粒径的减小而增大。采用三步光电发射模型对上述结果进行理论分析,表明阴极光电效率的提高主要由于阴极光电发射面积的增加和局域强电场导致的表面势垒降低。     

Observation of persistent α‐helical content and discrete types of backbone disorder during a molten globule to ordered peptide transition via deep‐UV resonance Raman spectroscopy

The molten globule (MG) state can aid in the folding of a protein to a functional structure and is loosely defined as an increase in structural disorder with conservation of the ensemble secondary structure content. Simultaneous observation of persistent secondary structure content with increased disorder has remained experimentally problematic. As a consequence, modeling how the MG state remains stable and how it facilitates proper folding remains difficult due to a lack of amenable spectroscopic techniques to characterize this class of partially unfolded proteins. Previously, deep‐UV resonance Raman (dUVRR) spectroscopy has proven useful in the resolution of global and local structural fluctuations in the secondary structure of proteins. In this work, dUVRR was employed to study the MG to ordered transition of a model four‐helix bundle protein, HP7. Both the average ensemble secondary structure and types of local disorder were monitored, without perturbation of the solvent, pH, or temperature. The MG to ordered transition is induced by stepwise coordination of two heme molecules. Persistent dUVRR spectral features in the amide III region at 1295–1301 and 1335–1338 cm⁻¹ confirm previous observations that HP7 remains predominantly helical in the MG versus the fully ordered state. Additionally, these spectra represent the first demonstration of conserved helical content in a MG protein. With successive heme binding, significant losses are observed in the spectral intensity of the amide III₃ and S regions (1230–1260 and 1390 cm⁻¹, respectively), which are known to be sensitive to local disorder. These observations indicate that there is a decrease in the structural populations able to explore various extended conformations with successive heme binding events. DUVRR spectra indicate that the first heme coordination between two helical segments diminishes exploration of more elongated backbone structural conformations in the inter‐helical regions. A second heme coordination by the remaining two helices further restricts protein motion. Copyright © 2013 John Wiley & Sons, Ltd.     

Interactions of gamma rays with undoped and Mn-doped sodium phosphate glasses

Ultraviolet and visible spectroscopic measurements were used to investigate prepared undoped and Mn-doped sodium phosphate glasses before and after successive gamma irradiation. The effects of both glass composition and MnO₂ content on the generation of radiation-induced defects were investigated. Undoped sodium phosphate glass shows strong UV absorption, which is attributed to the presence of trace iron impurities present in the raw materials. Mn-doped glasses reveal an additional visible broad band centered at about 500 nm due to Mn³⁺, which has recently been related to the ⁵E_g →⁵T_2g transition. The radiation-induced bands are correlated with the generation of liberated electron–hole pairs during the process of gamma irradiation and the possibility of photochemical reactions especially with trace iron impurities and manganese ions. The intensity and the position of the induced bands are observed to depend on the type and composition of glass, concentration of the dopant and also on the irradiation dose. Manganese ions when present in relatively higher content have been found to show a shielding behavior towards the effects of progressive gamma irradiation causing a retardation of the growth of the induced defects. Infrared and Raman spectra of the undoped and Mn-doped glasses were measured to investigate the structural phosphate groups present and the effect of MnO₂ on the network structure. An ESR investigation was carried out to confirm the state of manganese ions in the prepared sodium phosphate glasses.     

熔石英介质中强紫外激光自聚焦效应研究

 针对脉冲宽度约1 ns、波长为351 nm的三倍频紫外激光,定量分析了熔石英介质中的自聚焦长度、峰值光强与强紫外激光光束质量及环境条件等因素的关系,研究了产生紫外光非线性自聚焦效应的阈值条件。研究结果表明：在强紫外激光光束质量一定的前提下,可将B积分值定义为自聚焦的阈值条件;入射强紫外激光光束质量越差,在熔石英介质中产生自聚焦的阈值条件越低;即使对于空间分布均匀的理想光束,当空气中存在灰尘时,经过一段距离的传输后,在熔石英介质中将导致强紫外激光自聚焦效应的产生,且灰尘尺寸较大时的自聚焦效应较明显,自聚焦的阈值条件也相对较低。     

Ce3+-冠醚配合物的紫外有机电致发光

采用5d-4f跃迁的稀土Ce³⁺-冠醚配合物(Ce-二环己基并-18-冠-6,Ce-DC18C6)作为发光掺杂剂,4,4'-二(9-咔唑基)联苯(CBP)为基质,设计制备了紫外发光器件:ITO/CuPc/Ce-DC18C6:CBP/Bu-PBD/LiF/Al,首次观测到峰位于376nm的Ce³⁺离子的紫外电致发光。通过对比器件的EL谱与Ce³⁺-冠醚配合物薄膜的PL谱发现,EL光谱中有部分来自Ce³⁺配合物中的Ce³⁺离子的。这种5d-4f电子跃迁的掺杂质量分数为3%时,该UV-发光器件的最大辐射功率为13μW/cm²。     

防紫外线伞ATR-OMNI采样器的FTIR分析

本实验使用OMNI采样器，直接对伞面内外进行单反射ATR的分析，快速、简便、不破坏样品，得到分辨率很好的红外图谱，再配合红外谱库的谱图检索，可以分别得到匹配率95％以上的尼龙－6和聚酯型聚氨酯的分析结果。