HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women

Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method’s applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 µg/L, confidence interval (%CI): 95; 138) than in the supplement group (133 µg/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC–UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects.     

综合护理干预对妊娠高血压综合征患者血压及妊娠结局的影响

目的探讨综合护理干预对妊娠高血压综合征患者血压及妊娠结局的影响。方法选取2012年6月至2015年2月来江西省新余市中医院接受治疗的88例PIH患者作为研究对象。按照入院顺序抽签后随机分为实验组和对照组,对照组患者进行常规护理。实验组患者在对照组护理的基础上,进行综合护理干预。观察患者血压、分娩方式结局、胎儿结局。结果实验组护理后收缩压和舒张压均低于对照组和护理前(P0.05)。实验组早产、产后出血、胎盘早剥、剖宫产总发生率均低于对照组(P0.05)。实验组胎儿窘迫、新生儿窒息总发生率均低于对照组(P0.05)。结论综合护理干预能够降低妊娠高血压综合征患者的血压,改善妊娠结局。     

广州市孕妇头发微量元素含量测定

测定了286例不同孕周孕妇头发锌、铁、铜、钙、锰、铅含量,并进行了比较分析。结果表明,孕期上述元素的异常率分别为：40．21％、37．76％、15．04％、45．46％、19．58％、27．97％。不同孕周变化各有差异,以孕中期变化最为明显（除铜和锰外,P＜0．01）。提示妊娠期妇女有益微量元素缺乏的现象是很普遍的,妊娠中晚期事理调整膳食结构和营养物质的补充是必要的。     

正常妊娠与妊高征患者血清中8种必需微量元素水平的研究

７８例正常妊娠妇女和４０例妊高征患者血清中铁、锌、铜、锰、硒、铬、钴和镍的含量与健康未孕妇女比较，正常妊娠妇女和妊高征患者均是铜和锰的含量升高，铁、锌、硒、铬、钴和镍含量降低；妊高征患者与正常妊娠妇女比较，则是铁、铜和锰的含量升高，锌、硒、铬、钴和镍的含量降低。     

Real-time autocorrelator with enhanced temporal scan range for measurements of ultrashort laser pulses

A simple, low-cost light emitting diode-based quasi-real-time autocorrelator has been developed with two-fold enhancement in temporal scan range than conventional commercial autocorrelators. We use the technique of twin audio-speaker, but avoid the complex electronic circuitry to achieve two 180° out-of-phase ramp signals. Next, the use of pick-up coils, switching electronics and sum amplifier to generate a time marker signal for autocorrelation signal measurement is also dispensed with. The suitability of our setup is demonstrated by recording autocorrelation signals of 200 fs laser pulses from Nd:phosphate oscillator under different ramp signal voltages.     

Synthesis and characterization of Cu(II) complexes of pyrazine-2,3-dicarboxylicacid

Four copper(II) coordination polymers, {[Cu(pz(COO)₂)(H₂O)]₄·HBr}_n (1), {[Cu(pz(COO)₂)(NH₃)₂]·H₂O}_n (2), {[Cu₃H₂(pz(COO)₂)₄(H₂O)₃]·2H₂O}_n (3) and {[Cu₂(pz(COO)₂)₂(NH₃)₂(H₂O)₃][Cu(pz(COO)₂)(NH₃)(H₂O)₂][Cu(pz(COO)₂)(NH₃)(H₂O)]·2H₂O}_n (4) were synthesized using pyrazine-2,3-dicarboxylic acid, CuBr₂, 2-(2-aminoethylamino)ethanol/triethanol amine/ammonia in a methanol:water (1:1) solution, and the mixed ligand complexes were characterized by spectroscopic methods, thermal and elemental analysis, and magnetic susceptibility. Complexes 2 and 4 were also characterized by means of single crystal X-ray crystallography. The characterizations show that the complexes have polynuclear molecular structures, except for complex 2, and all of the complex structures form polymeric chains. Complex 4 has a pseudo-merohedral twin structure.     

Dietary Antioxidant Curcumin Mitigates CuO Nanoparticle-Induced Cytotoxicity through the Oxidative Stress Pathway in Human Placental Cells

The placenta is an important organ that maintains a healthy pregnancy by transporting nutrients to the fetus and removing waste from the fetus. It also acts as a barrier to protect the fetus from hazardous materials. Recent studies have indicated that nanoparticles (NPs) can cross the placental barrier and pose a health risk to the developing fetus. The high production and widespread application of copper oxide (CuO) NPs may lead to higher exposure to humans, raising concerns of health hazards, especially in vulnerable life stages, e.g., pregnancy. Oxidative stress plays a crucial role in the pathogenesis of adverse pregnancy outcomes. Due to its strong antioxidant activity, dietary curcumin can act as a therapeutic agent for adverse pregnancy. There is limited knowledge on the hazardous effects of CuO NPs during pregnancy and their mitigation by curcumin. This study aimed to investigate the preventive effect of curcumin against CuO NP-induced toxicity in human placental (BeWo) cells. CuO NPs were synthesized by a facile hydrothermal process and characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and photoluminescence techniques. We observed that curcumin did not induce toxicity in BeWo cells (1–100 µg/mL for 24 h), whereas CuO NPs decreased the cell viability dose-dependently (5–200 µg/mL for 24 h). Interestingly, CuO NP-induced cytotoxicity was effectively mitigated by curcumin co-exposure. The apoptosis data also exhibited that CuO NPs modulate the expression of several genes (p53, bax, bcl-2, casp3, and casp9), the activity of enzymes (caspase-3 and -9), and mitochondrial membrane potential loss, which was successfully reverted by co-treatment with curcumin. The mechanistic study suggested that CuO-induced reactive oxygen species generation, lipid peroxidation, and higher levels of hydrogen peroxide were significantly alleviated by curcumin co-exposure. Moreover, glutathione depletion and the lower activity of antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase) were effectively mitigated by curcumin. We believe this is the first report exhibiting that CuO-induced toxicity in BeWo cells can be effectively alleviated by curcumin. The pharmacological potential of dietary curcumin in NP-induced toxicity during pregnancy warrants further investigation.     

工程强度理论的发展和统一

简要总结了工程强度理论的发展和现况，指出存在的问题，然后提出了作者经３０年研究而创立并发展的双剪强度理论和统一强度理论．统一强度理论，一方面建立起现有各种强度理论之间的联系；同时也使以往各种只能适用于某一类材料的单一强度理论发展为可以适用于众多材料的统一强度理论；可以广泛适用于从金属到岩石、混凝土、土体等各类材料。最后通过二个实例，说明统一理论的应用。     

双芯光纤孤子开关及其势函数研究

根据耦合非线性薛定谔方程研究孤子在双芯光纤中的传输,讨论了两芯具有不同增益或损耗的双芯光纤孤子开关特性。并用势函数的概念解释孤子在双芯光纤中的传输与开关行为。