FIA-双流通池同时测定血清中的锌和铜

本文以2-(5-溴-2-吡啶偶氮)-5-二乙氨基苯酚(5-Br-PADAP)为显色剂,乳化剂OP为增溶剂,二乙基二硫代氨基甲酸钠(DDTC)为竞争络合剂,采用双流通池(一个流通池用来测定锌和铜络合物吸光度之和,另一个测定锌络合物吸光度),在一次进样中同时测定血清中锌和铜的含量。测定频率为45样/小时,测定铜和锌的线性范围分别为0.02~0.10μg/mL和0~0.12μg/mL。本法具有操作简单、快速、灵敏度高和易于推广等优点。     

Synthesis and characterization of Cu(II) complexes of pyrazine-2,3-dicarboxylicacid

Four copper(II) coordination polymers, {[Cu(pz(COO)₂)(H₂O)]₄·HBr}_n (1), {[Cu(pz(COO)₂)(NH₃)₂]·H₂O}_n (2), {[Cu₃H₂(pz(COO)₂)₄(H₂O)₃]·2H₂O}_n (3) and {[Cu₂(pz(COO)₂)₂(NH₃)₂(H₂O)₃][Cu(pz(COO)₂)(NH₃)(H₂O)₂][Cu(pz(COO)₂)(NH₃)(H₂O)]·2H₂O}_n (4) were synthesized using pyrazine-2,3-dicarboxylic acid, CuBr₂, 2-(2-aminoethylamino)ethanol/triethanol amine/ammonia in a methanol:water (1:1) solution, and the mixed ligand complexes were characterized by spectroscopic methods, thermal and elemental analysis, and magnetic susceptibility. Complexes 2 and 4 were also characterized by means of single crystal X-ray crystallography. The characterizations show that the complexes have polynuclear molecular structures, except for complex 2, and all of the complex structures form polymeric chains. Complex 4 has a pseudo-merohedral twin structure.     

Metabolic infrastructure of pregnant women with methylenetetrahydrofolate reductase polymorphisms: A metabolomic analysis

The aim of this study was to demonstrate the altered metabolic infrastructure of pregnant women with methylenetetrahydrofolate reductase (MTHFR) polymorphisms at first trimester and during delivery. Eight singleton pregnant women with MTHFR polymorphisms were compared with 10 normal pregnant women. Maternal blood samples were obtained twice during their pregnancy period (between the 11th and 14th gestational weeks and during delivery). Metabolomic analysis was performed using GC–MS. The GC–MS based metabolomic profile helped identify 95 metabolites in the plasma samples. In the MTHFR group, the levels of 1-monohexadecanoylglycerol, pyrophosphate, benzoin, and linoleic acid significantly decreased (P ˂ 0.05 for all), whereas the levels of glyceric acid, l -tryptophan, l -alanine, l -proline, norvaline, l -threonine, and myo-inositol significantly increased (P ˂ 0.01 for the first two metabolites, P ˂ 0.05 for the others) at 11–14 gestational weeks. Conversely, the levels of benzoin, 1-monohexadecanoylglycerol, pyruvic acid, l -proline, phosphoric acid, epsilon-caprolactam, and pipecolic acid significantly decreased in the MTHFR group, whereas metabolites such as hexadecanoic acid and 2-hydroxybutyric acid increased significantly in the study group during delivery. An impaired energy metabolism pathway, vitamin B complex disorders, tendency for metabolic acidosis (oxidative stress), and the need for cell/tissue support seem prevalent in pregnancies with MTHFR polymorphisms.     

Mechanism for the Pseudoelastic Behavior of FCC Shape Memory Nanowires

Pseudoelasticity and shape memory have been recently discovered in single-crystalline FCC nanowires of Cu, Ni, Au and Ag. The deformation mechanism responsible for this novel behavior is surface-stress-driven reorientations of the FCC lattice structure. A mechanism-based continuum model has been developed for the lattice reorientation process during loading through the propagation of a single twin boundary. Here, this model is extended to the nucleation, propagation and annihilation of multiple twin boundaries associated with the reverse reorientation process during unloading. The extended model captures the major characteristics of the loading and unloading behavior and highlights the dominating effect of the evolution of twin boundary structure on the pseudoelasticity.     

经阴道多普勒彩色超声联合血清人绒毛膜促性腺激素对子宫瘢痕妊娠的诊断价值分析

本研究拟探讨经阴道多普勒彩色超声联合血清人绒毛膜促性腺激素(β-HCG)对子宫瘢痕妊娠(CSP)的诊断价值。选取2018年4月~2019年4月于我院妇产科就诊的疑似子宫瘢痕妊娠患者56例作为研究对象,同时行阴道多普勒彩色超声和血清β-HCG水平检查,经手术和(或)病理组织学检查确诊。结果显示,CSP患者经阴道多普勒彩色超声特征征象包括子宫前壁下段肌层可见孕囊、孕囊与膀胱之间的子宫肌层变薄、孕囊周边高速低阻信号。CSP患者48 h血清β-HCG增加幅度低于非CSP患者(t=7.425,P=0.000)。48 h血清β-HCG增加幅度的ROC曲线下面积为0.919,最优界值为64.22%,此时诊断的敏感度为80.00%,特异性为88.90%。与手术和(或)病理检查相比,单一诊断和联合诊断的一致性均较好,且对CSP的诊断的敏感度、特异度、阳性预测值、阴性预测值、准确率比较,差异均无统计学意义(P>0.05)。两种检查手段可以互为参考,降低误诊率与漏诊率。     

人性化护理对药流术效果及术后阴道出血量的影响

目的研究并分析对药流终止妊娠患者使用人性化护理后对其术后阴道出血量的影响效果。方法收集药流终止妊娠患者共130例,根据平行、单盲、随机对照的设计原则分为对照组(65例)和观察组(65例),对照组接受常规护理,观察组结合人性化护理,将两组患者的引产成功率、不良反应发生率、术后阴道出血时间以及出血量进行观察和对比。结果观察组的引产成功率明显高于对照组,观察组的不良反应发生率显著低于对照组,观察组的术后阴道出血时间显著短于对照组,观察组术后阴道出血量显著低于对照组,P0.05。结论在药流终止妊娠患者的护理过程中,人性化护理能够显著提高药流术的效果,并对术后不良反应和阴道出血量、出血时间进行干预,值得推广应用。     

孕母中期血铅水平对妊娠结局影响的初探

为探讨孕中期铅负荷对孕妇分娩、新生儿神经行为发育的影响 ,随机抽取了 1 1 8例配对孕产妇及新生儿作为研究对象 ,采用石墨炉原子吸收法测定了其孕中期、产时脐带及新生儿静脉的血铅水平 ;比较了不同分娩方式的产妇血铅水平 ;采用儿童神经行为发育量表 (NABA)对出生2 4～ 3 6h新生儿进行了评价 ,并与其母孕中期、脐带血铅水平进行了相关分析 ;以孕中期母血铅c的第 2 5、 5 0、 75百分位 ,即 0 2 7μmol/L、 0 3 1 μmol/L、 0 3 6μmol/L为分界点 ,分为相对高铅组和低铅组 ,对其神经行为发育进行了比较。结果表明 ,剖宫产者血铅水平显著高于顺产者 ;母孕中期血铅值与新生儿行为能力、主动肌张力及总分呈显著负相关 (r =-0 2 96,-0 2 41 ,-0 3 89,P <0 0 5 ) :脐血铅值与行为能力及总分呈显著负相关 (r =-0 1 3 4,-0 2 76,P <0 0 5 ) :在分界点 0 3 1 μmol/L时 ,两组的总分评分开始出现显著性差异。提示低铅负荷影响孕妇分娩 ,孕中期的低血铅暴露 ( 0 3 1 μmol/L) ,对新生儿的神经行为发育产生不良影响。     

微量元素与妊娠并发症的关系

为探讨孕妇体内微量元素与妊娠并发症的关系,采用回顾性分析的方法,分析了住院分娩有妊娠并发症的孕妇体内微量元素的变化,其中妊高征、胎膜早破、胎儿宫内窘迫、FGR各5 0例,并随机选取同期住院分娩无任何合并症和并发症的孕妇5 0例作为对照组,测定其体内微量元素Zn、Fe、Cu的代谢情况,并探讨其相关性。结果表明,妊高征、胎膜早破、FGR、胎儿宫内窘迫孕妇的静脉血中Zn含量明显低于正常孕妇组,胎膜早破的孕妇血中Cu含量明显低于正常孕妇组(P <0 0 1 ) ,FGR、胎儿宫内窘迫的孕妇血中含Fe量也明显偏低,与正常孕妇组比较,差异有显著性意义。结论是妊娠并发症的出现与孕妇血中的微量元素代谢异常可能有一定的关系