Characterisation of a Simple Non‐Thermal Atmospheric Pressure Plasma Source for Biomedical Research Applications

The recent progress in plasma medicine as well as biomedical aspects of plasma physics over the last years have enhanced the need for experimental plasma devices which are capable of producing non‐thermal atmospheric pressure plasmas. These plasma sources are used for studying the effect of non‐thermal plasmas on biological samples of different nature. In this paper we present such an easy to build low cost apparatus that can be used for scientific as well as for educational purposes. Directions for the construction of the device are given and the basic plasma parameters are investigated. The characterisation of the experiment was done by electrical diagnostics for the measurements of the plasma potential in combination with optical emission spectroscopy. The latter is used for the determination of the excitation temperature of the plasma and the electron density. Furthermore the influence of the produced plasma on yeast cells is demonstrated. The produced plasma is characterised in different ways and it was found that the feeding gas has also a considerable impact on the radio frequency wave form of the source. It is also demonstrated that the constructed plasma source is capable of producing non‐thermal plasma that hinder cell growth of yeast colonies on a agar substrate. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

基于冠心病心肌缺血血瘀证小型猪血清核磁共振代谢组学的研究

通过左冠状动脉前降支放置Ameriod环制备冠心病慢性心肌缺血动物模型,并结合冠脉造影等方法确立中医血瘀证证候,采用现代核磁共振(NMR)和模式识别技术,分析冠心病心肌缺血血瘀证模型与假手术组小型猪4星期血清的共振氢谱,及相应代谢物谱群的变化,开展中医血瘀证候学的研究.结果显示,该方法制备的模型4星期后确立为稳定的冠心...     

HPLC-ICP/MS联用同时分析中药材中的多种形态砷

利用高效液相色谱-电感耦合等离子体质谱(HPLC-ICP/MS)对中药材中的6种砷的形态(三价砷(As(Ⅲ))、五价砷(As(Ⅴ))、二甲基砷酸(DMA)、甲基砷酸(MMA)、砷甜菜碱(AsB)和砷胆碱(AsC))进行了同时分析.采用1.2mol/,L HCl浸提,Hamilton PRP-X100阴离子交换色谱柱分离...     

离子色谱同时测定中草药中磷硫含量

应用离子色谱法同时测定了硫酸根和磷酸根的含量。用茶叶(GBW10016)、柑橘叶(GBW10020)和黄芪(GBW10028)标准物质来验证方法的准确性,测定值与标准值基本吻合。磷在0.01～100.0μg/mL,硫在0.01～60.00μg/mL范围内呈良好的线性关系(R2≥0.9993),磷和硫的检出限分别为0.0...     

QuEChERS-气相色谱法快速检测五十种中药材中九种有机氯农药残留的方法研究

建立了简便、快速测定五十种中药材中九种农药的多残留检测方法.以体积比95:4:1的乙腈-水-1%乙酸混合溶液作为提取剂,QuEChERS(Quick,Easy,Cheap,Effective,Rugged,Safe)方法进行净化,采用气相色谱法进行检测.结果表明:九种农药在1.0～100ng/mL范围内线性良好,3个添...     

Liquid‐Chromatography Quantitative Analysis of 20 Amino Acids after Derivatization with FMOC‐Cl and Its Application to Different Origin Radix isatidis

We developed a simple, rapid and reliable method for determination of 20 common amino acids based on derivatization with 9‐fluorenylmethyl chloroformate (FMOC‐Cl) and RP‐LC/UV, this method was first introduced into quantitative analysis of amino acids. The amino groups of amino acids were trapped with FMOC‐Cl to form amino acid‐FMOC‐Cl adducts which can be suitable for LC‐UV. Chromatographic separation was performed on a C₁₈ column with a mobile phase gradient consisting of acetonitrile and sodium acetate solution. This method was shown to be sensitive for 20 common amino acids. In the intra‐day precisions assay, the range of RSDs was 3.21‐7.67% with accuracies of 92.34‐102.51%; for the inter‐day precisions assay, the range of RSDs was 5.82‐9.19% with accuracies of 90.25‐100.63%. The results also indicated that solutions of amino acids‐FMOC‐Cl can be kept at room temperature for at least 24 h without showing significant losses in the quantified values. The validated method was successfully applied to the determination of major four kinds of amino acids in R. isatidis samples (Arg, Pro, Met and Val). The total content of amino acids in different origin R. isatidis was 13.32‐19.16 mg/g. The differences between R. isatidis samples were large using HCA.     

Advanced development in analysis of phytochemicals from medicine and food dual purposes plants used in China

The concept of "Let food be thy medicine and medicine be thy food" was widely accepted for thousand years. It is now well known that some foods and food components have beneficial physiological and psychological effects. In China, the Ministry of Health of the People's Republic of China announced 87 items of materials, including 81 plants, could be used as both medicine and food. Increasing consumer demand for safety and health benefits, it is of critical importance to have high-quality and comprehensive data on bioactive compounds (phytochemicals) in these materials. In this review, we summarized the advanced development (2006-2010) in analysis of phytochemicals from medicine and food dual purposes plants (MFDPP) used in China.     

西咪替丁治疗消化性溃疡与其它药物的合理应用

对西咪替丁治疗消化性溃疡与其它药物方面不良相互作用以及使用不当进行了分析，并且提出了合理应用之对策。     

稀土元素在中草药中的应用及其前景

本文介绍了稀土元素在中草药中的应用及其前景，探讨了稀土元素对中草药药效的影响，引用文献39篇。     

药源性铅中毒浅谈

铅为有毒微量元素，广泛存在于空气，土壤，水源中，药物在种植，生产过程中有可能带入铅毒，加上病人用药不当，极易造成药源性铅污染。