Improvement of the SOS/UMU Test for Preliminary Screning of Mutagens

Abstract

A short-term system to detect environmental mutagens is presented by using a plasmid (pSK1002) carrying a fused gene umuC′-′lacZ introduced into Salmonella typhimurium TA1535. Higher sensitivity, repeatability, and simplicity were achieved when (1) the culture volume taken from 2hrs after treatment with test compound was increased to 1.5ml diluted with distilled water to 2.5ml, (2) the 15μ 1 of 3% SDS solution saturated with chloroform was used as the disruptor of the cell membrane. and (3) an incubation -temperature of 37°C was used. At the above experimental conditions, the time required for the mutagen test was shortened to 3hrs. Based on the results of the present study, it seems that the improved SOS/umu test is useful for screening of mutagenic complex environmental mixtures.     

Development of a test strip based on DNA-functionalized gold nanoparticles for rapid detection of mercury(II) ions

A rapid,sensitive,selective and reliable strip assay based on DNA-functionalized gold nanoparticles for Hg²⁺ detection has been developed,with a detection limit 5 nmol/L.The measurement principle was based on thymine-Hg²⁺-thymine(T-Hg²⁺-T) coordination chemistry and streptavidin-biotin interaction.The major advantages of this assay are that results can be read visually without any instrument in less than 10 min and that it does not require any sample pretreatment.     

Elastoplastic stress analysis for cylindrical shell with flat strip imperfection

Based on unequidistant B-spline function, generalized spline subdomain displacement mode of rotational shell is obtained by taking double-direction interpolation of spline. The elastoplastic constitutive equation of shells is established by using the endochronic theory.According to the initial deflection theory of shells, the elastoplastic stress analysis of cylindrical shells with flat strip geometrical imperfection is studied. Numerical results show that the geometrical imperfection has a great effect on the stress distribution of shells.     

Silicon detector technology development in India for the participation in international experiments

A specific research and development program has been carried out by BARC in India to develop the technology for large area silicon strip detectors for application in nuclear and high energy physics experiments. These strip detectors will be used as pre-shower detector in the CMS experiment at LHC, CERN for π ⁰/λ rejection. The fabrication technology to produce silicon strip detectors with very good uniformity over a large area of ∼40 cm², low leakage currents of the order of 10 nA/cm² per strip and high breakdown voltage of >500 V has been developed by BARC. The production of detectors is already under way to deliver 1000 detector modules for the CMS and 90% production is completed. In this paper, research and development work carried out to develop the detector fabrication technology is briefly described. The performance of the silicon strip detectors produced in India is presented. The present status of the detector technology is discussed.      

Bipower-type estimation in a noisy diffusion setting

We consider a new class of estimators for volatility functionals in the setting of frequently observed Itō diffusions which are disturbed by i.i.d. noise. These statistics extend the approach of pre-averaging as a general method for the estimation of the integrated volatility in the presence of microstructure noise and are closely related to the original concept of bipower variation in the no-noise case. We show that this approach provides efficient estimators for a large class of integrated powers of volatility and prove the associated (stable) central limit theorems. In a more general Itō semimartingale framework this method can be used to define both estimators for the entire quadratic variation of the underlying process and jump-robust estimators which are consistent for various functionals of volatility. As a by-product we obtain a simple test for the presence of jumps in the underlying semimartingale.     

Theory of the transmission of metal strip gratings on a dielectric substrate application to submillimeter laser coupling

The advantages of supported metal strip gratings with respect to free-standing metal meshes are pointed out for applications as laser output couplers. A new, simple formula for the power transmission through the strip grating on a dielectric plate is derived from a line equivalent-circuit model. This formula is in good agreement with measurements performed on several samples, at different submillimeter wavelengths. An application to output couplers of far-infrared gas discharge lasers allows to improve the reliability of such lasers.x Unité associée au CNRS. U.A. 836.     

Pushing the envelope of the test functions in the Szegö and Avram–Parter theorems

The Szegö and Avram–Parter theorems give the limit of the arithmetic mean of the values of certain test functions at the eigenvalues of Hermitian Toeplitz matrices and the singular values of arbitrary Toeplitz matrices, respectively, as the matrix dimension goes to infinity. The question on whether these theorems are true whenever they make sense is essentially the question on whether they are valid for all continuous, nonnegative, and monotonously increasing test functions. We show that, surprisingly, the answer to this question is negative. On the other hand, we prove the two theorems in a general form which includes all versions known so far.     

魔芋总生物碱量的测定

用酸性染料比色法测定魔芋总生物碱量,以盐酸金刚烷胺计,在2—10μg/mL范围内线性关系良好。设计正交试验选择总生物碱的最佳提取条件为：料液比1：12,乙醇浓度85%,索氏提取4h。     

Singular densities,test particles and generalized Hamiltonian systems in general relativity

We derive the motion equations and the structure equations of neutral and charged test particles, starting from the gravitational field equations. The method consists in the application of conservation laws to singular tensor densities, which represent regions of strong matter concentration. Moreover, a Hamiltonian formulation of the particle equations is given, in the form of implicit differential equations generated by Hamiltonian Morse families.     

Large deviations for hitting times of a random walk in random environment on a strip

We consider a random walk in random environment on a strip, which is transient to the right. The random environment is stationary and ergodic. By the constructed enlarged random environment which was first introduced by Goldsheid （2008）, we obtain the large deviations conditioned on the environment （in the quenched case） for the hitting times of the random walk.