Optical biosensor for pharmaceuticals, antibiotics, hormones, endocrine disrupting chemicals and pesticides in water: Assay optimization process for estrone as example

Certain contaminants at trace concentrations in surface waters can have dramatic effects on the hormonal system of organisms in the aquatic environment. Therefore, immunoanalytical methods at a very low limit of detection (LOD) and a low limit of quantification (LOQ) are becoming more and more important for environmental analysis and especially for monitoring drinking water quality. Environmental monitoring of antibiotics, hormones, endocrine disrupting chemicals, and pesticides in real water samples (e.g. surface, ground or drinking water) with difficult matrices places high demands on chemical analysis. Biosensors have suitable characteristics such as efficiency in allowing very fast, sensitive, and cost-effective detection. Here we describe an assay optimization process with a fully automated immunoassay for estrone which resulted in a LOD below 0.20 ng L⁻¹ and a LOQ below 1.40 ng L⁻¹. In contrast to common analytical methods such as GC-MS or HPLC-MS, the biosensor used requires no sample pre-treatment and pre-concentration. The very low validation parameters for estrone are the result of the continuous optimization of the immunoassay. The basis of our sensitive assay is the antibody with a high affinity constant towards estrone. During the optimization process, we reduced the amount of antibody per sample and improved the chip surface modification. Finally, this proceeding led to a calibration routine with an amount of antibody of only 3.0 ng per sample (sample volume: 1.0 mL). The reduction of the amount of antibody per sample results in better validation parameters (LOD, LOQ, and IC₅₀), but this reduction leads to the current device-related limitation of the River Analyser (RIANA).For some endocrine disrupting compounds, no effect levels (NOELs) in the lower nanogram per liter range are reported. This defines the challenge, which analytical methods have to compete with and our RIANA instrument with its improved sensitivity for the detection of a single hormone in the lower nanogram per liter range is a powerful tool in aquatic analytics in addition to the common analytical methods.     

新一代永磁型磁共振成像系统及其医学应用

简述了世界磁共振成像技术的发展趋势,分析了永磁型共振成像系统的常见不足及其产生的物理原因,据此,提出了解决这些问题的方法,同时,介绍了解决这些问题之后开发出的新一代永磁型磁振成像系统Ｏｐｅｎ Ｍａｒｋ２０００的特点及其在医学上的应用。     

微波光子晶体的实验研究

制造了工作于微波波段的三角形光子晶体,并测量了光子晶体的透射谱和存在缺陷时的光子晶体的透射谱。在此基础上,研究了它的缺陷模,并讨论了光子晶体的潜在应用     

一种烟气冷却器的研制

介绍了某型烟气冷却器的设计思路,主要从结构的紧凑性、制冷系统的匹配等方面给予了阐述,通过实际应用说明了其设计的可行性,证明设计是成功的.     

连续值域信息系统的规则提取与知识约简

研究连续值域信息系统的规则提取和知识约简，给出规则提取的完整方法和2种新的知识约简的概念，讨论各种协调集之间的关系，探讨系统允许误差变化对规则提取的影响。     

THE RIEMANN-HILBERT BOUNDARY VALUE PROBLEM FOR THE MOISIL-THEODORSCO SYSTEM

This article studies the inhomogeneous Moisil-Theodorsco system in the space R3, gives the integral expression of its solution, proves the Holder continuity of the solution. Moreover the author studies the Riemann-Hilbert boundary value problem for the Moisil-Theodorsco system in a cylindrical domain of R3, and gives the solvability conditions and the integral expressions of solutions. The Holder continuity of the solutions is proved.     

ARMAX系统不外加输入激励的辨识

本文在不外加输入激励情况下，讨论了开环不稳定和非最小相位的ARMAX系统系数的一致估计．所用方法是用适应镇定的办法，使得闭环系统成为平稳可逆的ARMA过程，然后利用Yule-Walker方程给出闭环系统系数的一致估计，而把求开环系统系数的一致估计归结为解一组线性代数方程。     

A new family of iterative methods for solving system of nonlinear algebric equations

Homotopy perturbation method (HPM) is applied to construct a new iterative method for solving system of nonlinear algebric equations. Comparison of the result obtained by the present method with that obtained by revised Adomian decomposition method [Hossein Jafari, Varsha Daftardar-Gejji, Appl. Math. Comput. 175 (2006) 1–7] reveals that the accuracy and fast convergence of the new method.     

ON THE EXISTENCE OF GLOBAL GENERAL SOLUTIONS OF POLYNOMIAL SYSTEMS

ＯＮＴＨＥＥＸＩＳＴＥＮＣＥＯＦＧＬＯＢＡＬＧＥＮＥＲＡＬＳＯＬＵＴＩＯＮＳＯＦ　ＰＯＬＹＮＯＭＩＡＬＳＹＳＴＥＭＳＺＨＡＯＸＩＡＯＱＩＡＮＧ（赵晓强）（ＩｎｓｔｉｔｕｔｅｏｆＡｐｐｌｉｅｄＭａｔｈｅｍａｔｉｃｓ，ｔｈｅＣｈｉｎｅｓｅＡｃａｄｅｍｙｏ...     

非线性密度制约的Ⅱ类功能反应系统的定性分析

本文对具有非线性密度制约的Ⅱ类功能反应系统进行了定性分析,给出了平衡点全局渐近稳定和存在极限环的充分条件及生态解释,所得结果就其参数空间和相空间而言都是大范围的。