核酸工具酶辅助的信号放大技术在生物分子检测中的应用

核酸工具酶具有催化能力高、序列识别能力强、反应条件温和以及生物相容性好等优势,因而被广泛地应用于核酸、蛋白质和小分子等生物活性分子的分析检测。本文主要对核酸工具酶进行介绍,并对应用于生物分子检测的一些核酸工具酶辅助的信号放大技术进行较为全面的综述,并展望了该技术的应用前景。     

A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA–AuNPs probe

A novel electrochemical sensing strategy was developed for ultrasensitive and rapid detection of Salmonella by combining the rolling circle amplification with DNA–AuNPs probe. The target DNA could be specifically captured by probe 1 on the sensing interface. Then the circularization mixture was added to form a typical sandwich structure. In the presence of dNTPs and phi29 DNA polymerase, the RCA was initiated to produce micrometer-long single-strand DNA. Finally, the detection probe (DNA–AuNPs) could recognize RCA product to produce enzymatic electrochemical signal. Under optimal conditions, the calibration curve of synthetic target DNA had good linearity from 10 aM to 10 pM with a detection limit of 6.76 aM (S/N = 3). The developed method had been successfully applied to detect Salmonella as low as 6 CFU mL⁻¹ in real milk sample. This proposed strategy showed great potential for clinical diagnosis, food safety and environmental monitoring.     

光纤放大器中的受激布里渊散射阈值

从受激布里渊散射(SBS)耦合波理论出发,根据双包层光纤放大器中的受激布里渊散射阈值模型,理论仿真了信号带宽、纤芯直径、放大器增益对SBS阈值的影响,并从实验上研究了单频百纳秒脉冲信号在掺镱双包层光纤放大器中的受激布里渊散射现象。实验中输入脉冲信号重复频率1 Hz,脉宽200 ns,对不同的输入脉冲信号放大,前向放大脉冲在脉冲能量660 nJ、峰值功率3.3 W时出现畸变,产生后向SBS窄脉冲,达到了SBS阈值,实验计算的SBS阈值与理论分析结果基本一致。     

Electrochemical Signal Tracing by Glucose Oxidase and Ferrocene Dually Functionalized Gold Nanoprobe for Ultrasensitive Immunoassay

A glucose oxidase (GOD) and ferrocene (Fc) dually functionalized gold nanoprobe was simply prepared for electrochemical immunoassay. By combination with sandwich immunoreaction at a carbon nanotube (CNT)‐based immunosensor and signal tracing of the nanoprobe through the Fc‐mediated GOD‐catalytic reaction, a new electrochemical immunoassay method was successfully developed. Both the multi‐enzyme signal amplification of the nanoprobe and the electron transfer promotion of the CNTs modified on the immunosensor greatly enhanced the signal response. Thus this method showed excellent analytical performance including ultrahigh sensitivity, wide linear range as well as good specificity, reproducibility, stability and reliability for human IgG measurement.     

42.8 Gbit/s DPSK信号的波分复用传输实验

实现了42.8 Gbit/s 差分相移键控调制信号的三信道波分复用传输实验.传输链路为410 km的标准单模光纤,分为四个放大段,采用色散补偿光纤进行色散补偿和掺铒光纤放大器/分布式喇曼放大器混合放大方式.给出了差分相移键控信号及其解调后的信号在背对背和传输后的光谱和眼图(中路波长信号).在接收端使用单端检测,给出中路波长的差分相移键控信号背对背情况和传输后的误码率曲线,并与单信道传输时进行比较.经过传输后的中路信号的误码率可维持在1.0E-3左右.     

BBOⅠ类相位匹配光参量放大中群速失配的补偿

描述了在BBOⅠ类相位匹配的飞秒光参量放大（OPA）中，利用非共线结构和倾斜抽运光的脉 冲波面来完全补偿三波群速失配的方法.理论计算了三波群速匹配时，非共线角、相位匹配 角、抽运光的脉冲波面倾斜角随信号光波长的变化，并分析了对抽运光光斑尺寸的要求和对 空间走离长度的影响.结果表明，利用该方法不仅能够实现最大的参量带宽，而且能够完全 补偿飞秒OPA中三波的群速失配.此外，选取合适的抽运光光斑尺寸和非线性晶体的长度对提 高参量增益也至关重要. 关键词： 群速匹配 非共线相位匹配 脉冲波面倾斜 飞秒光参量放大