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61.
High‐affinity aptamers for important signal transduction proteins, i.e. Cdc42‐GTP, p21‐activated kinase1 (PAK1) and MRCK (myotonic dystrophy kinase‐related Cdc42‐binding kinase) α were successfully selected in the low micro‐ to nanomolar range using non‐systematic evolution of ligands by exponential enrichment (SELEX) with at least three orders of magnitude enhancement from their respective bulk affinity of naïve DNA library. In the non‐SELEX procedure, CE was used as a highly efficient affinity method to select aptamers for the desired molecular target through a process that involved repetitive steps of partitioning, known as non‐equilibrium CE of equilibrium mixtures with no PCR amplification between successive steps. Various non‐SELEX conditions including the type, concentration and pH of the run buffer were optimized. Other considerations such as salt composition of selection buffer, protein concentration and sample injection size were also studied for high stringency during selection. After identifying the best enriched aptamer pool, randomly selected clones from the aptamer pool were sequenced to obtain the individual DNA sequences. The dissociation constants (Kd) of these sequences were in the low micromolar to nanomolar range, indicating high affinity to the respective proteins. The best binders were also subjected to sequence alignment to generate a phylogenetic tree. No significant consensus region based on approximately 50 sequences for each protein was observed, suggesting the high efficiency of non‐SELEX for the selection of numerous unique sequences with high selectivity. 相似文献
62.
鲁剑锋 《中国光学与应用光学文摘》2011,(5)
介绍了无人机载设备的技术特点及机载小型图像处理器的组成。采用TI公司的数字信号处理器芯片为核心处理器,配合现场可编程逻辑器件(FPGA)与外部管理微控制单元(MCU)来实现目标数据的采集与处理,设计了适用于无人机光电载荷的超小型图像处理器系统。针对机载设备的特点,设计时充分考虑了体积、重量、功耗等要求。该系统已应用于多台套机载光电载荷中,工作稳定可靠,满足无人侦察机对捕捉和定位目标的要求。 相似文献
63.
基于小波变换和高斯拟合的在线谱图综合处理方法 总被引:1,自引:0,他引:1
微小型移动式现场在线检测技术是分析仪器发展的新领域。针对复杂工作环境中谱图存在强噪声干扰、谱峰重叠、不规则峰形等严重影响仪器的定性和定量准确度的瓶颈技术,提出了一种基于小波变换和高斯拟合相结合的谱图在线综合处理方法,用自研的仪器对甲苯和全氟三丁胺两种典型化合物的谱图进行了处理,并与实验室分析仪器普遍应用的算法进行了对比分析。结果表明,综合方法能够有效解决强噪声干扰、谱峰重叠、不规则峰形问题,提高仪器的定性和定量准确性,同时能够实现数据压缩,满足仪器的在线实时检测要求。综合方法处理甲苯特征峰的平均信噪比(SNR)较移动平滑方法提高了1.3倍,峰位误差ΔM降低了3.6倍,处理全氟三丁胺谱图的数据压缩比为197∶1。 相似文献
64.
Comparison of the performance of three ion mobility spectrometers for measurement of biogenic amines
The performance of three different types of ion mobility spectrometer (IMS) devices: GDA2 with a radioactive ion source (Airsense, Germany), UV-IMS with a photo-ionization source (G.A.S. Germany) and VG-Test with a corona discharge source (3QBD, Israel) was studied. The gas-phase ion chemistry in the IMS devices affected the species formed and their measured reduced mobility values. The sensitivity and limit of detection for trimethylamine (TMA), putrescine and cadaverine were compared by continuous monitoring of a stream of air with a given concentration of the analyte and by measurement of headspace vapors of TMA in a sealed vial. Preprocessing of the mobility spectra and the effectiveness of multivariate curve resolution techniques (MCR-LASSO) improved the accuracy of the measurements by correcting baseline effects and adjusting for variations in drift time as well as enhancing the signal to noise ratio and deconvolution of the complex data matrix to their pure components. The limit of detection for measurement of the biogenic amines by the three IMS devices was between 0.1 and 1.2 ppm (for TMA with the VG-Test and GDA, respectively) and between 0.2 and 0.7 ppm for putrescine and cadaverine with all three devices. Considering the uncertainty in the LOD determination there is almost no statistically significant difference between the three devices although they differ in their operating temperature, ionization method, drift tube design and dopant chemistry. This finding may have general implications on the achievable performance of classic IMS devices. 相似文献
65.
A set of original, analytical equations useful for theoretical calculation of the electron paramagnetic resonance (EPR) signal intensity are presented for the multitude of sample shapes, which range from point-like, line-like, planar, rectangular, cubical, circular, cylindrical, spherical to an irregularly shaped sample. The samples can be situated at any available position within the prescribed part of the microwave cavity (a central cylinder of diameter 11 mm and length 23.5 mm, in either a Bruker single TE102 or double TE104 rectangular cavity, with the modulation coils situated in the left and right side cavity walls, which is connected to a X-band, field-modulated CW Bruker EPR spectrometer). The theoretical computations of EPR signal intensity can be used in the computer simulations in which: (i) the EPR signal intensity profiles are constructed; (ii) the optimal sample positions in the cavity to give a maximum value of signal intensity are found; (iii) the errors associated with sample positioning within the cavity when compared to a second sample of a different size, shape or position are studied. 相似文献
66.
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68.
为实现水下中低频声信号的探测识别,通过研究水下多声源相干探测信号的特征,理论上给出了相干探测信号频谱混叠情况下的特征表达式,并提出了一种基于Hilbert变换的信号解调处理方法,实现了水下多声源相干探测信号频谱混叠情况下各声源发声频率的解调.该方法将探测信号经过滤波平滑处理之后进行Hilbert变换,得到信号的解析形式,然后对解析信号模值的平方进行二次滤波平滑等处理,分离混叠在一起的频带,将得到的信号进行频谱分析,根据频移值计算得到水下各个声源的发声频率.在光学暗室下搭建激光相干探测系统,对2~6kHz的水下声信号进行实验,实验结果表明,该方法可以有效分离探测信号中混叠在一起的信号频带,并准确提取各水下声信号的发声频率,频率提取重复性不大于2.5Hz. 相似文献
69.
In this review,the most recent progresses in the field of fluorescence signal amplification strategies based on DNA nanotechnology for miRNA are summarized.The types of signal amplification are given and the principles of amplification strategies are explained,including rolling circle amplification(RCA),catalytic hairpin assembly(CHA),hybridization chain reaction(HCR)and DNA walker.Subsequently,the application of these signal amplification methods in biosensing and bioimaging are covered and described.Finally,the challenges and the outlook of fluorescence signal amplification methods for miRNA detection are briefly commented. 相似文献
70.
飞机驾驶杆LVDT(线性位移差分变压器)传感器信号测试校正在航空测控研究领域具有十分重要的意义,提出了一种针对某型飞机驾驶杆LVDT传感器信号精确调理校正方法;介绍了飞机驾驶杆LVDT传感器原理,分析了AD598 LVDT调理芯片的使用局限,利用最小二乘法设计了精确调理校正方法;基于VC++2010开发环境,利用高精度的模拟校正源产生模拟激励校正信号,设计了驾驶杆LVDT多通道软件自校正方法,它可以有效提高解调电路的线性度和对称性,克服了AD598常规调理电路的使用局限,从而实现驾驶杆LVDT信号精确检测;理论分析与数值试验验证了所提方法的正确性和有效性。 相似文献