排序方式: 共有67条查询结果,搜索用时 15 毫秒
61.
Zhiqiang Tan 《Journal of computational and graphical statistics》2017,26(1):54-65
Consider the two problems of simulating observations and estimating expectations and normalizing constants for multiple distributions. First, we present a self-adjusted mixture sampling method, which accommodates both adaptive serial tempering and a generalized Wang–Landau algorithm. The set of distributions are combined into a labeled mixture, with the mixture weights depending on the initial estimates of log normalizing constants (or free energies). Then, observations are generated by Markov transitions, and free energy estimates are adjusted online by stochastic approximation. We propose two stochastic approximation schemes by Rao–Blackwellization of the scheme commonly used, and derive the optimal choice of a gain matrix, resulting in the minimum asymptotic variance for free energy estimation, in a simple and feasible form. Second, we develop an offline method, locally weighted histogram analysis, for estimating free energies and expectations, using all the simulated data from multiple distributions by either self-adjusted mixture sampling or other sampling algorithms. This method can be computationally much faster, with little sacrifice of statistical efficiency, than a global method currently used, especially when a large number of distributions are involved. We provide both theoretical results and numerical studies to demonstrate the advantages of the proposed methods. 相似文献
62.
1984年中国化学会第一届全国生物无机化学会议在武汉召开,标志了我国生物无机化学研究的开始。作为我国生物无机化学研究的重要开创者之一,王夔教授积极倡导并开展了细胞生物无机化学研究。本文以王夔教授的研究为主,侧记了我国细胞层次的生物无机化学的某些重要进展。 相似文献
63.
Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) is an effective technique for analysis of complex samples with offering rapid, efficient separation in combination with accurate mass measurement and tandem mass spectrometry (MS/MS). This paper exploits this technique to identify the alkaloids in corydalis yanhusuo, an important antalgic Traditional Chinese Medicine (TCM). The mass spectral fragmentation behavior of one tertiary alkaloid and two quaternary alkaloids was studied in detail. Low-abundance product ions of tertiary and quaternary alkaloids were investigated and compared between each other. Sixteen alkaloids were screened out by using a systematic screening method developed in our laboratory; structures of eight therein were identified by characteristic UV absorption spectrum and positive ion mode of Q-TOF-MS/MS; and two of them were discovered for the first time in corydalis yanhusuo to our knowledge. This research demonstrates the potential of UPLC-Q-TOF-MS in structural characterization and identification of components in traditional Chinese herbal medicines. 相似文献
64.
《Analytical letters》2012,45(11):1929-1936
Abstract A highly sensitive spectrophotometric method for determination of palladium by the new chromogenic reagent (BPT) has been developed. In the presence of cetyltrimethylammonium bromide(CTMAB), palladium reacts with BPT to form a stable yellow complex in HAc-NaAc buffer solution of pH 4.6-5.6 at 294.4 nm with a molar absorptivity of 2.46 x 105 l mol?1 cm?1. The method is simple, rapid and sensitive. Trace amounts of palladium in mineral and catalyst samples have been satisfactorily determined by the method. 相似文献
65.
Blas J Rivera-Sagredo A Ferritto R Espinosa JF 《Magnetic resonance in chemistry : MRC》2004,42(11):950-954
The combination of 1D and 2D high-resolution magic angle spinning NMR experiments led to the assignment of the proton and carbon resonances for several disubstituted benzoates bound to a polystyrene resin through a Wang linker. It is shown that the signal corresponding to the methylene protons of the linker can be utilized to monitor the solid-phase reactions and determine the loading of the compounds on the resin. 相似文献
66.
Suzuki coupling reaction for the solid-phase preparation of 5-substituted nicotinic acid derivatives
Joan-Carles Fernàndez Laia Solé-Feu Natalia de la Figuera Fernando Albericio 《Tetrahedron letters》2005,46(4):581-585
The application of the Suzuki coupling reaction to the preparation of small combinatorial libraries using 5-bromonicotinic acid as a scaffold onto three different types of solid support (Wang, Rink, and BAL resin) is described. 相似文献
67.
Magnetic beads (MBs) are versatile tools in the separation of nucleic acids, proteins and other biomacromolecules, their complexes and cells. In this article recent application of MBs in electrochemical biosensing and particularly in the development of DNA hybridization sensors is reviewed. In these sensors MBs serve not only for separation but also as a platform for optimized DNA hybridization. A hybridization event is detected separately at another surface, which is an electrode. The detection is based either on the intrinsic DNA electroactivity or on various kinds of DNA labeling, including chemical modification, enzyme tags, nanoparticles, electroactive beads, etc., greatly amplifying the signals measured. In addition to DNA hybridization, other kinds of biosensing in combination with MBs, such as DNA-protein interactions, are reviewed. 相似文献