Improved preparative electrochromatography column design

Improved chromatography column fittings were developed for the efficient and reliable application of an electric field to a preparative chromatography column (a process termed electrochromatography). The improved fittings contained electrodes in close proximity to the column packing media and allowed uniform electric fields to be applied. Membranes in the fittings prevented mixing of the electrode and the column eluent buffers. The membranes prevented gases and electrolytic products generated in the electrode chamber from entering the column eluent buffer. An electrode buffer solution was pumped through the electrode chamber to a large external container. The circulation of buffer through the electrode chamber removed the gases and electrolytic products and ensured a uniform electric field by helping to maintain a constant buffer composition. The membranes prevented macromolecules being separated on the column from coming in contact with the electrodes.     

Measuring colloidal forces with the magnetic chaining technique

In 1994 Leal Calderon et al. (Phys. Rev. Lett. 72, 2959 (1994)) introduced the magnetic chaining technique to directly probe the force-distance profile between colloidal particles. In this paper, we revisit this approach in two ways. First, we describe a new experimental design which allows us to utilize sample volumes as low as a few microliters, involving femtomoles of surface active macromolecules. Secondly, we extensively describe the characterization and preparation of the magnetic colloids, and we give a quantitative evaluation of performance and resolution of the technique in terms of force and interparticle separation.     

On practical aspects of Laser Guide Stars

The Gemini Observatory has developed an extensive Adaptive Optics (AO) program, including Classical AO, Laser Guide Star (LGS) AO, Multi-Conjugate AO (MCAO), extreme AO (eXAO) and Ground Layer AO (GLAO). Most of these instruments use one or several LGSs. A laser has been in operation at Gemini since May 2005. Most of the laser related systems (beam transport, launch, safety systems) have been developed in house. These are major components, requiring a development effort not to be underestimated. In this article, we propose to share the Gemini experience in terms of practical issues and calibration requirements associated with the use of lasers in AO. To cite this article: F. Rigaut, C. d'Orgeville, C. R. Physique 6 (2005).     

Gas‐flow assisted ion transfer for mass spectrometry

Methods and devices that use gas flows to collect ions and transfer them over long distances for mass spectrometric analysis have been developed. Gas flows derived from the ionization source itself or provided by means of additional pumping were used to generate a laminar flow inside cylindrical tube. Hydrodynamic simulations and experimental tests demonstrate that laminar flow can transfer ions over long distance. The typical angular discrimination effects encountered when sampling ions from ambient ionization sources are minimized, and the sampling of relatively large surface areas is demonstrated with desorption electrospray ionization (DESI). Ion transfer over 6 m has been achieved and its application to multiplexed chemical analysis is demonstrated on samples at locations remote from the mass spectrometer. Copyright © 2012 John Wiley & Sons, Ltd.     

Twin column chromatography for industrial-scale decontamination processes

A frontal chromatographic unit was devised consisting of a column-detector-column array. The unit is either equipped with identical columns (identical twins) or with columns of varying length (fraternal twins). Due to the finite nature of the columns, a prerun is formed at the column walls following the same regularities as the main stream. These regularities are used for the identification of the process termination below the detection limits of the monitor. For the implementation, a clear preference is given to the employment of fraternal twins, as the feed assay can be integrated into the separation process.     

High-performance liquid chromatography-tandem mass spectrometry with a new quadrupole/linear ion trap instrument

The use of a new hybrid quadrupole/linear ion trap known as the Q TRAP offers unique benefits as a LC-MS-MS detector for both small and large molecule analyses. The instrument combines the capabilities of a triple quadrupole mass spectrometer and ion trap technology on a single platform. Product ion scans are conducted in a hybrid fashion with the fragmentation step accomplished via acceleration into the collision cell followed by trapping and mass analysis in the Q3 linear ion trap. This results in triple quadrupole fragmentation patterns with no inherent low molecular mass cutoff. In-trap fragmentation is also possible in order to provide triple MS (MS3) capabilities. There are also several scan modes that are not possible on conventional instruments that enable identification of analytes within complex biological matrixes for subsequent high sensitivity product ion scans. This report will describe the new hybrid instrument and the principles of operation, and also provide examples of the unique scan modes and capabilities of the Q TRAP for LC-MS-MS detection in metabolism identification.     

Methodological developments in overpressured-layer chromatography

Summary Since its introduction around 1977, OPLC has found widespread application and its methodology has been further developed. A possible development is gradient elution. Different continuous development methods (e.g. with eluent changeover, multistep gradient) and the possible system configurations for their execution are discussed. The advantages of the newly developed continuous development techniques are compared to earlier data. The most versatile method is demonstrated on lipid samples derived from human plasma, giving separation of six lipid classes and intra-class separation of the most polar classes in the same run.     

X射线荧光光谱分析

本文评述了我国在2005年至2006年X射线荧光光谱,包括粒子激发的X射线光谱的发展和应用,内容包括仪器研制、激发源、探测器、软件、仪器改造、仪器维护和维修、样品制备技术、分析方法研究和应用.     

Translating IΔ0 + exp Proofs into Weaker Systems

The purpose of this paper is to explore the relationship between IΔ₀ + exp and its weaker subtheories. We give a method of translating certain classes of IΔ₀ + exp proofs into weaker systems of arithmetic such as Buss' systems S₂. We show if IE_i (exp) ⊢ A with a proof P of expind‐rank(P) ≤ n + 1where all (∀ ≤: right) or (∃ ≤: left) have bounding terms not containing function symbols, then Sⁱ ₂ ⊇ IE_i,2 ⊢ Aⁿ. Here A is not necessarily a bounded formula. For IOpen(exp) we prove a similar result. Using our translations we show IOpen(exp) ⊊ IΔ₀(exp). Here IΔ₀(exp) is a conservative extension of IΔ₀ + exp obtained by adding to IΔ₀ a symbol for 2 ^x to the language as well as defining axioms for it.     

A new luminescence detection and stimulation head for the Risø TL/OSL reader

A new automated Detection And Stimulation Head (DASH) has been developed for the Risø TL/OSL luminescence reader to provide easy access to new technologies, new signals and new measurement methods. The automated DASH includes a filter changer and a detector changer that makes it possible to change stimulation filters (4 × 4 filter combinations possible) and detectors (3 detectors possible) as part of a measurements sequence. The new automated DASH with dedicated driver electronics does not affect the use of other attachments, and can be retrospectively fitted to existing Risø TL/OSL readers.