桂郁金与桂莪术挥发油的色谱指纹图谱比较

应用化学计量学方法对中药桂郁金与桂莪术挥发油的色谱指纹图谱进行了比较分析。对样品特征峰数据进行奇异值分解,提取第一主成分抽象光谱后,以固定色谱窗口尺寸的另一样品数据构造正交投影阵,用提取的抽象光谱进行投影,移动窗口获取投影残差长度,判定两样品中有无相同的化学成分。经比较,桂郁金和桂莪术的指纹图谱中分别有11和7种化学成分单独存在,两者共有的化学成分有27种。     

Quantitative analysis of acankoreoside A and acankoreagenin in the leaves of Schefflera octophylla and Schefflera actinophylla using pressurized liquid extraction and high‐performance liquid chromatography coupled with evaporative light scattering detection

A rapid method based on pressurized liquid extraction followed by high‐performance liquid chromatography coupled with evaporative light scattering detection was firstly developed for the quantitative analysis of two bioactive triterpenoids (acankoreoside A and acankoreagenin) in the leaves of Schefflera octophylla and Schefflera actinophylla. The analysis was performed on an Agilent Zorbax SB‐Aq column (4.6 × 50 mm, 3.5 μm) with gradient elution of 0.1% formic acid and acetonitrile. Calibration curves of two analytes showed good linearity (R² > 0.9990) within the tested ranges. This novel method is simple, rapid and accurate, and the results of quantification showed that contents of each investigated compound is significant high in natural S. octophylla (6.36–14.83%), which indicated that natural S. octophylla as potential medicinal resource. Furthermore, hierarchical clustering analysis based on the typical peaks of acankoreoside A and acankoreagenin from the 17 tested samples showed that natural and cultured Schefflera species were in different clusters, which could provide a means of discriminating between Schefflera species from different origins. Thus, acankoreoside A and acankoreagnin could be selected markers for quality control of S. octophylla and S. actinophylla.