丹参活血化瘀活性成分的靶标

丹参活血化瘀活性成分主要有丹参素、迷迭香酸、丹参酮等化合物,这些化合物具有某些共同的化学结构特征.运用靶分子信息学平台,实现丹参共同化学结构特征与已知结构的蛋白质的结合能力的系统化研究,并进一步探索丹参活血作用的机理.结果表明,丹参活血化瘀活性成分主要通过调控人体内肾上腺素类激素的代谢网络,在一定程度上抑制肾上腺素类激素的分解代谢,形成积累,从而对心血管疾病具有治疗效果.丹参活血化瘀活性成分的主要靶标是苯乙醇胺N-甲基转移酶、儿茶酚O-甲基转移酶和单胺氧化酶A.     

A novel Salvialactomine from the callus culture of Salvia santolinifolia Boiss

A novel compound Salvialactomine (1) along with two other unusual occurring natural products Pentatriacontanoic acid 1, 3-dihydroxypropyl ester (2) and 5-Methylflavone (3) were isolated from the callus of Salvia santolinifolia Boiss. Callus was initiated on MS medium containing NAA (0.5 mg/L) and further sub-cultured on MS medium supplemented with NAA with BA (0.5 + 1.5 mg/L). The structures of isolated compounds were determined by using mass spectrometry, 1D, and 2D–NMR techniques. Compounds 1, and 3 were tested for two different cancer cell lines, i.e. Hela (Cervical cancer cell) and PC-3 (Prostate cancer cells). IC₅₀ was found as > 30 using Doxorobicin (0.912 ± 0.12 μmol L^?1) as a standard.     

Orthogonal array design for the optimization of supercritical fluid extraction of tanshinones from Danshen

Different from the west medicine, the therapeutic effect of the traditional Chinese medicine is usually based on multifarious essential components or the combination of them instead of only one component. In this paper, a novel supercritical fluid extraction (SFE) method has been developed for extracting tanshinones (dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone II(A)) from Danshen, the dried root of Salvia miltiorrhiza. Various experimental conditions were investigated to optimize the SFE. Under the appropriate conditions, extracting at 40 MPa, and 50 degrees C and with CO(2) flow rate of 25 L/h for 1 h, SFE can achieve a better yield as well as the recoveries of the tanshinones than the conventional extraction using methanol. Moreover, the target compounds were analyzed by HPLC with a C(18) RP column by gradient elution using ACN and water as mobile phase at a flow rate of 1.0 mL/min and with UV detection at 270 nm. Four calibration equations were then established and good linear relationships were shown (r(2) >0.999) in the investigated concentration range. The recoveries, measured at three concentration levels, varied from 97.2 to 103.8%. The method provided in this article could be applied as an improved quality control method for Danshen products.     

Polyphenolic contents and antioxidant activities of aerial parts of Salvia multicaulis from the Iran flora

Abstract

Folk medicine sometimes involves the use of salvia species for therapeutic purposes. Polyphenols with the highest amounts included rosmarinic acid (7.358mg/g), catechin (1.5?mg/g), vanillin (1.00?mg/g), chlorogenic acid (0.53?mg/g), quercetin (0.16?mg/g), and p-coumaric acid (0.015). Furthermore, the results showed that S. multicaulis has a high content of total phenol (4.39?mg/g) and DPPH activity (8.44?mg/g). Salvia multicaulis could be potentially used as a medicinal plant because of its antioxidant activity and polyphenol content.     

丹参木质素及其单体含量的测定

本文建立了一种准确、快速的检测丹参中木质素及其单体含量的方法.采用Klason法和紫外分光光度法分别对丹参根和茎中酸不溶性木质素(Klason木质素)和酸溶性木质素含量进行了测定;运用硫代酸解法并结合气相色谱-质谱法(GC-MS)分别对丹参根和茎中各木质素单体组成进行了分析.结果显示,总木质素在丹参根和茎中的含量分别为...     

Splendidins A – C,Three New Clerodane Diterpenoids from Salvia splendens

Three new clerodane diterpenoids, splendidins A–C ( 1 – 3 , resp.), were isolated from Salvia splendens, together with six known ones. Their structures were elucidated by extensive spectroscopic analysis. Splendidin C ( 3 ) was the first diterpenoid glucoside reported from this plant. These compounds were evaluated for their cytotoxic activity; however, none of them were cytotoxic.     

Gas chromatographic–mass spectrometric analysis of volatiles obtained by four different techniques from Salvia rosifolia Sm., and evaluation for biological activity

Four different isolation techniques, conventional hydrodistillation (HD), microwave-assisted hydrodistillation (MWHD), microdistillation (MD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the aerial parts of Salvia rosifolia Sm. by gas chromatography and gas chromatography coupled to mass spectrometry. HD and MWHD techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar essential oils. α-Pinene (15.7–34.8%), 1,8-cineole (16.6–25.1%), β-pinene (6.7–13.5%), β-caryophyllene (1.4–5.0%) and caryophyllene oxide (1.4–4.4%) were identified as major constituents of this Turkish endemic species. Besides, the hydrodistilled oil of S. rosifolia was evaluated for antibacterial, antifungal, anticancer, antioxidant and cytotoxic activities. The hydrodistilled oil of S. rosifolia showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) with a MIC value of 125 μg/mL. Other human pathogenic microorganisms (Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Salmonella typhimurium, Staphylococcus epidermidis, Candida albicans) were also inhibited within a moderate range (MIC = 125–1000 μg/mL). Antifungal activity of the oil was also observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. No cytotoxicity was observed for S. rosifolia oil up to 25 mg/mL against malignant melanoma, epidermal, ductal and ovary carcinoma.     

丹参有效成分体外抗氧化活性比较

研究了丹参水溶性和脂溶性成分体外抗氧化活性。以既可以测定水溶性成分,又可以测定脂溶性成分抗氧化活性的DPPH(1,1-Diphenyl-2-picrylhydrazyl)和TEAC(Trolox equivalent antioxidant capacity)法来对丹参的两类主要有效成分进行抗氧化活性测定。采用紫外-可见分光光度法进行分析,以维生素C为阳性对照,首次进行了丹参水溶性和脂溶性成分体外抗氧化活性比较。结果表明:丹参的水溶性成分具有较好的清除DPPH·、ABTS+·(2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium+·)的活性,而脂溶性成分仅显示出很弱的清除DPPH·和ABTS+·的活性。     

Antihyperglycemic and Lipid Profile Effects of Salvia amarissima Ortega on Streptozocin-Induced Type 2 Diabetic Mice

Salvia amarissima Ortega was evaluated to determinate its antihyperglycemic and lipid profile properties. Petroleum ether extract of fresh aerial parts of S. amarissima (PEfAPSa) and a secondary fraction (F6Sa) were evaluated to determine their antihyperglycemic activity in streptozo-cin-induced diabetic (STID) mice, in oral tolerance tests of sucrose, starch, and glucose (OSTT, OStTT, and OGTT, respectively), in terms of glycated hemoglobin (HbA1c), triglycerides (TG), and high-density lipoprotein (HDL). In acute assays at doses of 50 mg/kg body weight (b.w.), PEfAPSa and F6Sa showed a reduction in hyperglycemia in STID mice, at the first and fifth hour after of treatment, respectively, and were comparable with acarbose. In the sub-chronic test, PEfAPSa and F6Sa showed a reduction of glycemia since the first week, and the effect was greater than that of the acarbose control group. In relation to HbA1c, the treatments prevented the increase in HbA1c. In the case of TG and HDL, PEfAPSa and F6Sa showed a reduction in TG and an HDL increase from the second week. OSTT and OStTT showed that PEfAPSa and F6Sa significantly lowered the postprandial peak at 1 h after loading but only in sucrose or starch such as acarbose. The results suggest that S. amarissima activity may be mediated by the inhibition of disaccharide hydrolysis, which may be associated with an α-glucosidase inhibitory effect.