Direct determination of reserpine in urine using excitation-emission fluorescence combined with three-way chemometric calibration methodologies

The concentration of reserpine in urine was directly and quantitatively measured by using the excitation-emission fluorescence (EEM) combined with three-way calibration methodologies. Two calibration methods are based on the alternating trilinear decomposition (ATLD) and the self-weighted alternating trilinear decomposition (SWATLD) algorithms, respectively. These chemometric methodologies have the second-order advantage, which is the ability to get accurate concentration estimates of interested analyte(s) even in the presence of uncalibrated interferences. The satisfactory results on spiked urine samples are obtained, when the component number was chosen to 3 (N = 3) for both the methods. This experiment is easily carried out without time-consuming and complicated pretreatment. It has proved that the three-way calibration methodologies based on ATLD and SWATLD can be feasible to directly quantify the medical content of reserpine in urine. __________ Translated from Chemical Journal of Chinese Universities, 2007, 28 (5): 827–830 [译自: 高等学校化学学报]     

三维荧光二阶校正方法用于体液和细胞培养基中五味子甲素含量的快速测定

采用三维激发发射荧光光谱结合自加权交替三线性分解(SWATLD)二阶校正方法, 对人体液样(血浆样及尿液样)和细胞培养基样中五味子甲素的含量进行了直接快速定量分析. 在血浆背景、尿液背景和细胞培养基背景共存下, 当分析体系的组分数分别选择2时, 用SWATLD二阶校正方法获得相应五味子甲素的平均回收率分别为(100.4±1.6)%, (100.5±6.3)%和(103.6±4.5)%. 实验结果表明, 此方法不仅能够较好地解决这些复杂分析体系因背景内源荧光性物质与待分析物光谱严重重叠所引起的难分辨的问题, 还可以用于直接快速准确定量分析.