全文获取类型
收费全文 | 178篇 |
免费 | 8篇 |
国内免费 | 25篇 |
专业分类
化学 | 150篇 |
力学 | 2篇 |
综合类 | 1篇 |
数学 | 5篇 |
物理学 | 53篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 6篇 |
2020年 | 3篇 |
2019年 | 7篇 |
2018年 | 2篇 |
2017年 | 8篇 |
2016年 | 8篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 12篇 |
2012年 | 16篇 |
2011年 | 17篇 |
2010年 | 14篇 |
2009年 | 26篇 |
2008年 | 16篇 |
2007年 | 16篇 |
2006年 | 12篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 6篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
排序方式: 共有211条查询结果,搜索用时 15 毫秒
41.
Phage contamination is a very serious and unavoidable problem in modern fermentation industry.It is necessary to develop sensitive and rapid phage detection methods for the early detection of phage contamination.In the present work,a real-time,rapid,specific and quantitative phage T4 detection method based on surface plasmon resonance(SPR) technique has been introduced.Escherichia coli was immobilized onto the preformed MPA self-assembled monolayer(SAM) through the widely used EDC/NHS cross-linking reaction as the recognition element.The bacteria immobilization was verified efficiently through the electrochemical measurements and fluorescence microscopy observations.The specific adsorption was much stronger than the non-specific adsorption of phage T4 binding to the biosensor surface modified by E.coli,and the latter could be neglected.The detection sensitivity reached 1×10 7 PFU/mL within 10 min.Within the experimental phage concentrations,the linear correlation between the SPR response and the phage concentration was good.The results suggest that the SPR technique is a potentially powerful tool for the phage or other virus detections,as a label-free,real-time,and rapid method. 相似文献
42.
Yang Yang Xiao-Chao Xue Xiao-Feng Jin Li-Jun Wang Yin-Lin Sha Zhong-Jun Li 《Tetrahedron》2012,68(35):7148-7154
Ternary core/shell CdSeS/ZnS-QDs coated with N-acetyl lactosamine was prepared as a fluorescent probe to study the interactions of N-acetyl lactosamine and galectin-3. The synthesis of N-acetyl lactosamine was achieved through the ‘azidoiodoglycosylation’ method. The amount of ligand coated on QDs was determined by 1H NMR and ICP-OES. The interactions between carbohydrates and galectin-3 were measured using SPR. The results revealed that the affinity of galectin-3 with di- and multivalent N-acetyl lactosamine increased 20 and 184-fold, respectively. The prepared glyco-QDs could be used as an efficient fluorescent probe to study carbohydrates and galectin-3 interactions. 相似文献
43.
Preparation of Anti—Cardiac Troponin I Monoclonal Antibodies and Their Characterization with Surface Plasmon Resonance Biosensor 总被引:1,自引:0,他引:1
WEI Jing-yan LIU Xia SONG Da-qian BU Li-sha HU Xing MU Ying ZHANG Han-qi LUO Gui-min ZHANG Gui-zhen DING Jia-Hua WANG Wei-zhong JIN Qin-han 《高等学校化学研究》2003,19(2):183-189
IntroductionIn fast and slow skeletal and cardiac muscles,troponin I,the inhibitory protein of the troponin-tropomyosin complex,exists in three isotype formsencoded by three separated genes.The amino acidsequences of the two skeletal and one cardiac Tn Iforms( s Tn I and c Tn I,respectively) exhibit40 %dissimilarity[1] .Moreover,human cardiac Tn I has31 additional residues on the N - terminal end,which do not exist in skeletal forms,thus it pro-vides a high potential for obtaining cardiac-… 相似文献
44.
45.
讨论了光纤表面等离子体波传感器的工作原理,并对将其用于监测以甲基橙为代表的环境污水降解过程的可行性进行了探讨。用光纤SPR传感器监测了50 mL初始浓度为30 mg·L-1的甲基橙原溶液在降解过程浓度的变化,对降解过程中光纤SPR传感器的光谱进行了详细的分析;同时采用紫外-分光光度计对降解过程中溶液浓度的变化进行了监测,并对2种方法所测的数据进行了分析对比。结果表明,光纤SPR同常规方法的测量结果一致,随着降解时间的增加,甲基橙溶液的吸光度和浓度逐渐减小,光纤SPR传感器的共振波长逐渐发生蓝移,同初始标定的甲基橙原溶液共振光谱比较,说明甲基橙逐渐被降解,且在2 h内降解率达到73%,说明用光纤SPR传感器监测污水降解过程是完全可行的。研究结果不仅为环境污水降解过程提供了一种新的监测方法,同时促进了我国SPR传感技术与环保监测研究结合,为光纤SPR技术走向实用,并最终实现产业化积累了经验。 相似文献
46.
近年来,表面等离子共振(SPR)现象、由于在金属纳米材料研究以及在作为灵敏检测不同化学和生化物种信号的报告器方面有着特殊的应用价值,因而受到广泛的关注.本文较详细的介绍了表面等离子共振现象的形成机制,特别对如何利用本方法作为一种新型的物种检测报告器的有关问题做了较系统的讨论. 相似文献
47.
利用生物传感芯片质谱法(BIA/MS)对微球蛋白及其抗体的相互作用进行分析和鉴定.将微球蛋白抗体偶联到芯片上,让微球蛋白溶液流过芯片表面,然后使用“三明治”结构的微再生方法把结合的微球蛋白从芯片上洗脱下来,再对其进行酶解及质谱鉴定,在10-15mol水平得到了明确的结果. 相似文献
48.
G. V. Krylova A. M. Eremenko N. P. Smirnova S. Eustis 《Theoretical and Experimental Chemistry》2005,41(6):365-370
We have studied the photocatalytic activity of porous silicas (silica gel, mesoporous sol-gel films) modified by benzophenone
molecules, in the reaction of reduction of gold from tetrachloroaurate ions. Stable colloids of nanosized gold were obtained
as a result of irradiating aqueous alcoholic solutions of HAuCl4·3H2O in the presence of the photocatalyst SiO2-BP using as the stabilizers: a colloidal solution of silica (Ludox) or the surfactant sodium dodecyl sulfate (SDS). We have
studied the effect of the stabilizer on the kinetics of the photoreduction reaction, and also on the shape and size of the
nanoparticles formed.
__________
Translated from Teoreticheskaya i éksperimental’naya Khimiya, Vol. 41, No. 6, pp. 348–353, November–December, 2005. 相似文献
49.
Hideyoshi Abe Akira Kenmoku Noriyuki Yamaguch Kenjiro Hattori 《Journal of inclusion phenomena and macrocyclic chemistry》2002,44(1-4):39-47
Bi-galactose-branched cyclodextrins (Bi-Gal-CDs)(1–6) having different spacer arm lengths between two terminal galactoses were foundto have the optimum length for association with PNA lectin. Also, the inclusioninteraction of the drug depended on the length of the spacer arms. The dual association of thesecompounds was quantitatively evaluated by SPR and compared to the other oligosaccharide-branchedCDs (Scheme 1). The number and the length of the spacer arm are important for theassociation both with the lectin and drug for the purpose of targeting drug-delivery systems.The association constants K of bi-Gal-CD (2) with rat liver cells showed a 60 timeshigher association than with PNA. Direct evidence of the association between PNA andbi-Gal-CD (2) was characterized by AFM observations. The results obtained strongly suggested a method to find a new design for the targeting drug carrier. In order toincrease the association with the cell, a sufficient spacer arm length is necessary for the effectivedual recognition of the oligosaccharide-CDs. In order to increase the inclusion of thedrug, the CD structure of a multi-saccharide branch is necessary. 相似文献
50.
Dr. Jinxing Li Jun Matsumoto Prof. Dr. Li‐Ping Bai Prof. Dr. Asako Murata Prof. Dr. Chikara Dohno Prof. Dr. Kazuhiko Nakatani 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(42):14881-14889
The development of small molecules that can recognize specific RNA secondary and tertiary structures is currently an important research topic for developing tools to modulate gene expression and therapeutic drugs. Expanded CUG trinucleotide repeats, known as toxic RNA, capture the splicing factor MBNL1 and are causative of neurological disorder myotonic dystrophy type 1 (DM1). Herein, the rational molecular design, synthesis, and binding analysis of 2,9‐diaminoalkyl‐substituted 1,10‐phenanthroline (DAP), which bound to CUG trinucleotide repeats, is described. The results of melting temperature (Tm) analyses, surface plasmon resonance (SPR) assay, and electrospray spray ionization time‐of‐flight (ESI‐TOF) mass spectrometry showed that DAP bound to r(CUG)9 but not to r(CAG)9 and r(CGG)9. The dual luciferase assay clearly indicated DAP bound to the r(CUG)n repeat by affecting the translation in vitro. 相似文献