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超氧化物歧化酶[Superoxide d ismutase,SOD,(EC.1.15.1.1)]是广泛存在于生物体内的超氧阴离子自由基(·O2-)的天然清除剂,在机体保护方面起重要作用。在对SOD进行的一系列研究中,发现SOD的存在与生物体抗衰老、抗肿瘤、营养状况、免疫性疾病、炎症及辐射防护[1]有关。许多动植 相似文献
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BACKGROUND: The formation of native disulfide bonds between cysteine residues often limits the rate and yield of protein folding. The enzyme protein disulfide isomerase (PDI) catalyzes the interchange of disulfide bonds in substrate proteins. The two -Cys-Gly-His-Cys- active sites of PDI provide a thiol that has a low pKa value and a disulfide bond of high reduction potential (Eo'). RESULTS: A synthetic small-molecule dithiol, (+/-)-trans-1,2-bis(2-mercaptoacetamido)cyclohexane (BMC), has a pKa value of 8.3 and an Eo' value of -0.24 V. These values are similar to those of the PDI active sites. BMC catalyzes the activation of scrambled ribonuclease A, an inactive enzyme with non-native disulfide bonds, and doubles the yield of active enzyme. A monothiol analog of BMC, N-methylmercaptoacetamide, is a less efficient catalyst than BMC. BMC in the growth medium of Saccharomyces cerevisiae cells increases by > threefold the heterologous secretion of Schizosaccharomyces pombe acid phosphatase, which has eight disulfide bonds. This effect is similar to that from the overproduction of PDI in the S. cerevisiae cells, indicating that BMC, like PDI, can catalyze protein folding in vivo. CONCLUSIONS: A small-molecule dithiol with a low thiol pKa value and high disulfide Eo' value can mimic PDI by catalyzing the formation of native disulfide bonds in proteins, both in vitro and in vivo. 相似文献
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Debojyoti Lahiri Tuhin Bhowmick Bhabatosh Banik Reema Railkar Suryanarayanarao Ramakumar Rajan R. Dighe Akhil R. Chakravarty 《Polyhedron》2010
Dicobalt(II) complexes [{(B)CoII}2(μ-dtdp)2] (1–3) of 3,3′-dithiodipropionic acid (dtdp) and phenanthroline bases (B), viz. 1,10-phenanthroline (phen in 1), dipyrido[3,2-d:2′,3′-f]quinoxaline (dpq in 2) and dipyrido[3,2-a:2′,3′-c]phenazine (dppz in 3), have been prepared, characterized and their photo-induced anaerobic DNA cleavage activity studied. The elemental analysis and mass spectral data suggest binuclear formulation of the complexes. The redox inactive complexes have magnetically non-interacting dicobalt(II) core showing magnetic moment of ∼3.9 μB per cobalt(II) center. The complexes show good binding propensity to calf thymus DNA giving Kb values within 4.3 × 105–4.0 × 106 M−1. Thermal melting and viscosity data predict DNA groove binding and/or partial intercalative nature of the complexes. The complexes show significant anaerobic DNA cleavage activity in green light under argon atmosphere possibly involving radical species generated from the disulfide moiety in a type-I pathway. The DNA cleavage reaction under aerobic medium in green light is found to involve hydroxyl radical species. The dppz complex 3 exhibits significant photocytotoxicity in HeLa cervical cancer cells with an IC50 value of 2.3 μM in UV-A light of 365 nm, while it is essentially non-toxic in dark giving an IC50 value of >200 μM. A significant reduction of the dark toxicity of the organic dppz base (IC50 = 8.3 μM in dark) is observed on binding to the cobalt(II) center while essentially retaining its photocytotoxicity in UV-A light (IC50 = 0.4 μM). 相似文献
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Miloš R. Filipović Dr. Alaric C. W. Koh Dr. Stéphane Arbault Dr. Vesna Niketić Dr. Andrea Debus Ulrike Schleicher Dr. Christian Bogdan Dr. Manon Guille Dr. Fréderic Lemaître Dr. Christian Amatore Dr. Ivana Ivanović‐Burmazović Dr. 《Angewandte Chemie (International ed. in English)》2010,49(25):4228-4232