高光谱成像的垃圾分类识别研究

高光谱成像因光谱分辨率高、图谱合一、可实现快速无损检测等特点现已广泛应用于农业、医学、遥感等领域。现有的对可回收生活垃圾检测与分类的方法,都存在检测时间长,分类效率低,而大量多种垃圾无法同时快速分拣等问题。考虑到不同类别的生活垃圾由于其主要组成分子结构的差异,对不同波长的光有不同的吸收特性。高光谱图像在记录待分类垃圾的空间信息的同时,可以获得垃圾对不同波长的光的反射率光谱信息,通过建立识别分类模型对反射率光谱信息进行分析可以实现对高光谱图像中待分类垃圾的识别与分类。收集常见纸质、塑料、木质三种材料的可回收的垃圾样本,包括塑料瓶、食品包装袋、塑料玩具(饰品)碎片、一次性筷子、雪糕棒、木制家具碎片、木制包装盒、废旧课本、广告纸、办公用纸等多种物品共30个样本,进行清洗和裁剪处理,避免样本表面污渍对样本反射率产生影响。利用高光谱成像系统采集样本在近红外(780～1 000 nm)范围内的高光谱图像,其中18个样本做训练样本集,12个样本做测试样本集。对采集的样本图像数据做预处理,包括去噪声以及黑白校正反演反射率信息等处理;通过主成分分析(PCA)方法对训练样本集感兴趣区域(ROI)进行分析,提取到的特征波段为795.815,836.869,885.619,916.409,929.239,934.37,957.463,972.858和988.253 nm;在特征波段下分别提取这三种类别垃圾的参考光谱,通过光谱角度填图法(SAM)对测试样本ROI区域内提取的测试样本点集在特征波段下与参考光谱进行匹配,由匹配程度进行样本点归类,分析结果表明,测试样本集中纸制样本(A类别)、塑料样本(B类别)、木制样本(C类别)的分类准确度分别为100%,98%和100%,测试样本点集整体的分类准确度为99.33%;通过Fisher判别方法分析训练样本集得出判别函数式和判别准则,对测试样本点集分类,评价结果为A,B和C类样本分类准确度分别为100%,100%和97%,测试样本点集整体分类准确度为99%。通过SAM和Fisher两种判别方法对测试样本集的光谱图像进行目标物的检测与分类,结果表明,利用SAM判别方法在可回收垃圾的高光谱图像中实现检测与分类有更高的分类准确度,可达到99.33%。同时,也验证了使用高光谱成像进行可回收垃圾快速分类的科学性以及可行性,对未来系统化、机械化、智能化地解决生活中可回收垃圾的分类具有一定的实用意义。     

Interfacial kinetic enhancement of metal ion adsorption on binary mixed self-assembled monolayers

The adsorption of metal ions, a type of surface reaction on binary mixed self-assembled monolayers (SAMs) on a gold surface composed of 1,6-hexanedithiol (HDT) with 11-mercaptoundecanoic acid (MUA), was monitored by in situ surface plasmon resonance (SPR) measurements. The differential SPR reflectance (ΔR) enables the kinetics of adsorption of Pt²⁺ on the mixed SAMs to be investigated. Unlike single HDT SAM, kinetic analyses of the mixed SAMs showed that the rate of adsorption of Pt²⁺ was enhanced and that it was highly dependent on the fraction of MUA present. These SPR measurements suggest that the adsorption rate of metal ions can be readily manipulated simply by using mixed SAMs.     

Thiolated cyclodextrin self-assembled monolayer-like characterized with secondary ion mass spectrometry

In the work the focus is on the preparation of self-assembled monolayer-like films consisting of thiolated cyclodextrin on gold substrate and a characterization by using secondary ion mass spectrometry. The short (1 min) and long (1 h) time preparations of self-assembled monolayer-like films, resulting in submonolayer and monolayer regimes, are investigated, respectively. The observed species of thiolated cyclodextrin (M as molecular ion) self-assembled monolayer-like films are assigned to three groups: Au_xH_yS_z clusters, fragments with origin in cyclodextrin molecule associated with Au, and molecular ions. The group of Au_xH_yS_z (x = 2-17, y = 0-2, z = 1-5) clusters have higher intensities than other species in the positive and even more in negative mass spectra. Interestingly, the dependence between the number of Au and S atoms shows that with the increasing size of Au_xH_yS_z clusters up to 11 Au atoms, the number of associated S atoms is also increasing and then decreasing. Molecular species as (M−S+H)Na⁺, (M+H)Na⁺, AuMNa⁺, (M₂−S)Na⁺, and M₂Na⁺ are determined, and also in cationized forms with K⁺. The intensities of thiolated cyclodextrin fragments at the long time preparation are approximately 10 times higher than the intensities of the same fragments observed at the short time. The largest observed ions in thiolated cyclodextrin self-assembled monolayer-like films are AuM₂ and Au₂M. The thiolated cyclodextrin molecular ions are compared with hexadecanethiol molecular ions in the form of Au_xM_w where the values of x and w are smaller for thiolated cyclodextrin than for hexadecanethiol. This result is supported with larger, more compact, and more stabile thiolated cyclodextrin molecule.     

Ammonium Ion Optical Sensor Formation and Characterization of a Self‐Assembled Thiazole Containing Dibenzo‐18‐Crown‐6 Monolayer toward Developing Ammonium Ion‐Sensing Interface

Abstract

This letter demonstrates a selective NH₄ ⁺ detection using a synthesized thiazole benzo crownether ethylamine‐lipoic acid conjugate (1) monolayer. A self‐assembled monolayer (SAM) of 1 was formed on Au surface and well characterized by atomic‐force microscopy, Fourier transform infrared reflection absorption spectroscopy and cyclic voltammetry (CV). A good selectivity for NH₄ ⁺ sensing was observed in a range of 1.0×10^?1 to 1.0×10^?6 M by surface plasmon resonance.     

Modulation of cyanobacterial photosystem I deposition properties on alkanethiolate Au substrate by various experimental conditions

We present results from atomic force microscopy (AFM) images indicating various experimental conditions, which alter the morphological characteristics of self-assembled cyanobacterial PS I on hydroxyl-terminated self-assembled alkanethiolate monolayers (SAM/Au) substrates. AFM topographical images of SAM/Au substrates incubated in solutions containing different PS I concentrations solubilized with Triton X-100 as the detergent reveal large columnar aggregates (～100 nm and hence, much taller than a single PS I trimer) at high PS I concentrations. Depositions from dilute PS I suspensions reveal fewer aggregates and relatively uniform surface topography (～10 nm). Confocal fluorescence microscopy analysis of fluorescently tagged PS I deposited on to SAM/Au substrates using electric field and gravity driven techniques reveal preliminary indications of directionally aligned PS I attachments, besides corroborating a uniform monolayer formation, for the former deposition method. The complex attachment dynamics of PS I onto SAM substrates are further investigated from the AFM images of PS I/SAM/Au substrates prepared under different experimental conditions using: 1) PS I isolated as monomers and trimers 2) adsorption at elevated temperatures, and 3) different detergents with varying pH values. In each of the cases, the surface topology indicated distinct yet complex morphological and phase characteristics. These observations provide useful insight into the use of experimental parameters to alter the morphological assembly of PS I on to SAM substrates en route to successful fabrication of PS I based biohybrid photoelectrochemical devices.     

Immobilization of histidine-tagged proteins on electrodes

The development of new enzyme immobilization techniques that do not affect catalytic activity or conformation of a protein is an important research task in biotechnology including biosensor applications and heterogeneous reaction systems. One of the most promising approaches for controlled protein immobilization is based on the immobilized metal ion affinity chromatography (IMAC) principle originally developed for protein purification. Here we describe the current status and future perspectives of immobilization of His-tagged proteins on electrode surfaces. Recombinant proteins comprising histidine-tags or histidine rich native proteins have a strong affinity to transition metal ions. For metal ion immobilization at the electrode surface different matrices can be used such as self-assembled monolayers or conductive polymers. This specific technique allows a reversible immobilization of histidine-tagged proteins at electrodes in a defined orientation which is an important prerequisite for efficient electron transfer between the electrode and the biomolecule. Any application requiring immobilized biocatalysts on electrodes can make use of this immobilization approach, making future biosensors and biocatalytic technologies more sensitive, simpler, reusable and less expensive while only requiring mild enzyme modifications.     

Mapping of powdery mildew using multi-spectral HJ-CCD image in Beijing suburban area

Powdery mildew is one of the most serious diseases, which has a significant impact on the production of winter wheat. As an effective alternative to traditional sampling methods, remote sensing can be a useful tool in disease detection. This study examines the potential of a moderate resolution multispectral satellite image in disease monitoring at regional scale. At the suburban area around Beijing, a large size ground survey sample (n = 90) and the corresponding HJ-CCD image were acquired at the grain filling stage of winter wheat. A number of spectral features were found to be sensitive to powdery mildew through an independent t-test. Based on these spectral features, classification models were established using both spectral information divergence (SID) and spectral angle mapper (SAM), respectively. The results showed that the overall accuracies of disease identification and severity estimation were moderate. The estimation of normal and seriously infected samples yielded higher accuracies than slightly infected samples. The single phase HJ-CCD can only be used for locating the infected areas of powdery mildew, whereas is unable to discriminate the severity levels of disease. The presence of several stressors and disturbances other than disease is a possible reason of the unsatisfactory performance of disease monitoring models. Therefore, the integration of multi-phase onboard data and some relevant ancillary data is necessary to improve the accuracy and reliability of disease monitoring at regional scale.     

A convenient synthesis of long-chain 4-substituted benzyloxycarbonyl alkanethiols for the formation of self assembled monolayers on metal substrates

New 4-substituted benzyl esters of 16-mercaptohexadecanoic acid were prepared by developing a practicable synthetic procedure and using readily available staring materials. The compounds synthesized have been characterized by NMR, MS, IR spectra and elemental analysis. The mercapto derivatives are precursors for the formation of self-assembled monolayers on metal substrates. Dedicated to Professor Dr. Manfred Hesse on the occasion of his 70 ^th birthday     

Quantitative analysis of mixed self-assembled monolayers using ToF-SIMS

The spacing of chemical functional groups on self-assembled monolayers (SAMs) plays an important role in controlling the density of biomolecules in biochips and biosensors. In this sense, a mixed SAM made of two different terminal groups is a useful organic surface since spacing can be easily controlled by changing a relative mole fraction in a mixture solution. In this study, an acetylene-OCH₂O(EG)₃(CH₂)₁₁S-S(CH₂)₁₁(EG)₃OCH₂O-propene (Eneyne) SAM and mixed SAMs made by a mixture of (S(CH₂)₁₁(EG)₃OCH₂O-acetylene)₂ (Diyne) and (S(CH₂)₁₁(EG)₃OCH₂O-propene)₂ (Diene) were produced on gold substrates and measured by using ToF-SIMS. The secondary ion yield ratio of [Au·S(CH₂)₁₁(EG)₃OCH₂O-acetylene]⁻ to [Au·S(CH₂)₁₁(EG)₃OCH₂O-propene]⁻ was measured for each mixed SAM and plotted as a function of the mole fraction of Diyne to Diene in a SAM solution. The ion yield ratio of a mixed SAM produced from a solution with a mole fraction of 0.5 (i.e., 1:1 mixture) was 0.3, which corresponded well to the ion yield ratio measured from an Eneyne SAM. A time-dependent experiment of Eneyne SAM formation and immersion experiment of Eneyne SAM into Diyne solution or into Diene solution were performed. The relative ion yield ratio of 0.3 was due to a different secondary ion formation and not due to the difference in the amount of adsorbates on the surface, nor to the different binding strengths onto the gold surface. Our study shows that a mixed SAM with well-controlled spacing can be produced and quantified by using the ToF-SIMS technique.