低剂量氯化钐对大鼠血清激素水平的影响

应用放射免疫法测定了经腹腔注射、灌胃入尾静脉注射等３种不同方式，隔日给预寺鼠低剂量氯化钐０．０５ｍｇ／ｋｇ，一个月后，测试血清中生长激素，甲状腺素Ｔ３、Ｔ３及胰岛素水平。结果显示各实验组动物血清中生长激素、胰岛素水平均明显增高，甲状腺素Ｔ４及腹腔注射增高，其余两组无明显变化。     

固相复合抗体放射免疫分析法测定人体SOD的含量

本文描述了固相复合抗体放射免疫分析法测定人血清和红血球中超氧化物歧化酶的含量，该法灵敏度为０．５ｎｇ，非特异为２．０±０．６％，批内误差在３．４％～４．２％（ｎ＝９）之间，批间误差在７．０％～７．５％（ｎ＝６）之间，经测定，人血清正常值为２９１±７９（ｘ±ｓ）ｎｇ／ｍＬ，红血球正常值为８６１±９５（ｘ±ｓ）×１０＾－６Ｈｂ。该法灵敏度高、特异性好，简便，快速，适于分析大量样品。     

Specific N-terminal ACTH-radioimmunoassay after plasma extraction

Conclusions The ACTH-antiserum used here has a high N-terminal specificity, which allows a measurement of biologically active ACTH after plasma extraction for investigations of the function of the hypothalamo-pituitary-adrenal axis in various diseases. This assay is not suitable in our hand to measure paraneoplastic production of biologically inactive peptides of the ACTH-LPH family in plasma from patients with carcinomas but without clinical signs of ectopic ACTH-production. To answer this special question other specific radioimmunoassays (e.g. CLIP, LPH) must be performed.Supported by Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 51)     

A Spectrofluorimetric Method For Renin. Comparison With Bioassay And Radioimmunoassay

Abstract

A spectrofluorimetric method for protein estimation based on the use of l-4-diamino-2-3-dich1oro-anthraquinone as a fluorogenic reagent is presented and applied to renin study. The method is optimized and the variables that influence the development of the fluorescence are studied. Data obtained by this method for Renal Renin Activity (RRA) are compared with those obtained by Bioassay (BA) and Radioinmunoassay (RIA), finding that this spectrofluorimetric method is a good one related to Radioinmunoassay.     

A Radioimmunoassay for Norethindrone. II. Plasma Levels in Hypertensive and Nomotensive Oral Contraceptive Users

Abstract

A sensitive radioimmunoassay for measurement of norethindrone (N) in blood plasma has been developed. The coefficient of variation within the assay and between assays was 7 and 13.5% respectively. The procedural blanks were negligible, and recovery was approximately 81.8%. Blood plasma levels of N in 20 normotensive and 6 hypertensive women who were ingesting oral contraceptives (OCs) were measured, and the results indicate that the subjects who became hypertensive while taking OCs had significantly higher levels of N compared with normotensive OC users (p < .001).     

Radioimmunoassay of Testosterone in Murine Plasma

Abstract

This report describes a radioimmunoassay method for measuring unconjugated plasma testosterone (T) and its application to the measurement of T in murine plasma. The parameters of assay reliability, sensitivity, and accuracy were established. Di-hydrotestosterone, the only steroid which showed significant cross-reaction with the testosterone antibody used, was not found in significant quantities in male mice of 19 inbred strains examined. This suggested that chromatographic separation of T from other plasma steroids was unnecessary for the measurement of T in males of this species. Biological levels of T in normal adult male mice of 19 inbred strains showed a range of 0.64 to 21.9 ng/ml plasma and a mean of 5.10 ng/ml. Castration reduced testosterone levels to about 4% of the normal level; castration plus adrenalectomy reduced testosterone to levels below the sensitivity of this method. These investigations demonstrated the validity and reliability of this method for measuring uncanjugated plasma T in male mice.