Methodische Untersuchungen zur radioimmunologischen Bestimmung von 3,4-Dimethoxyphenyl?thylamin im Urin

Ohne Zusammenfassung

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Methodical investigations on the determination of 3,4-dimethoxyphenylethylamine in urine by radioimmunoassay

     

Radioimmunoassay of Plasma Corticosterone

Abstract

This report describes a radioimmunoassay method for the measurement of unconjugated corticosterone (B) in plasma. Utilizing an anti-Cortisol antiserum cross reacting with B combined with a one step celite microcolumn chromatographic system, we have measured this latter steroid accurately in small aliquots of plasma from both male and female subjects. The plasma levels of B obtained by this method agreed well with levels observed in adult men and women, using other techniques. During a menstrual cycle studied, wide fluctuations were observed with levels ranging from 1, 3 to 14, 4 ng/ml. Adrenal suppression decreased the levels of B below 1 ng/ml. The mineralocorticoid B is predominantly ACTH -dependent.     

HPLC-RIA: A convenient alternative for the specific estimation of multiple steroid hormones

Conclusion The outlined technique of combining automatic HPLC and RIA has been proved to be a practicable and specific method for the estimation of multiple adrenal steroids from different body fluids. It is equivalently suited for the simultaneous and specific assessment of any other steroid groups.

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HPLC-RIA: Eine praktische Alternative für die spezifische Bestimmung von mehreren Steroidhormonen aus einer Probe

     

A Radioimmunoassay for Serum and Urine Aldosterone by Celite Column Chromatography

Abstract

A relatively simple and rapid radioimmunoassay (RIA) for the measurement of aldosterone in serum and urine has been developed. The method involves extraction of 1 ml of serum or urine (after acid hydrolysis) with dichloromethane, followed by partition chromatography on celite microcolumns prior to RIA. Dextran coated charcoal is used for separation of free from antibody-bound aldosterone. The method is very sensitive, blanks are negligible and recovery is approximately The%. 80 coefficient of variation is 4. 3% (within assay) and 10. 4% (between assay). When known amounts of aldosterone were added to serum and urine pools containing low endogenous levels of this steroid recovery was quantitative. Aldosterone concentrations measured under various physiological conditions were in agreement with published data. Up to 150 samples can be assayed by 1 technician during 5 working days.     

Radioimmunoassay of Plasma 17-Hydroxypregnenolone

Abstract

This report describes a radioimmunoassay method for the measurement of unconjugated 17-hydroxypregnenolone (17-⁵δ-P) in plasma. Utilizing a highly specific 17-⁵δ-P antiserum combined with a one step celite microcolumn chromatographic system, we have measured this steroid accurately in small aliquots of plasma from both male and female subjects. The plasma levels of 17-⁵δ-P obtained by this method agreed well with levels observed in adult men and women, using two other techniques. Compared with the latters, however, the present method offered a tenfold greater sensitivity and a better precision.     

Simulataneous Measurement of Plasma Dehydroepiandrosterone and 16a-Hydroxydehydroepiandrosterone by Radioimmunoassay

Abstract

Combining a simple chromatographic system on celite microcolumns with partially specific antisera as binding reagents, simultaneous radioimmunoassay of dehydroepiandrosterone and 16a-hydroxydehydroepiandrosterone could be performed on the same aliquot of plasma. These steroids have been measured in plasma samples obtained from subjects of both sexes under various conditions.     

Ein Radioimmunoassay (RIA) zur Bestimmung von menschlichem Lysozym (Muramidase) im Serum, Urin und Liquor

Ohne Zusammenfassung

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A human lysozyme radioimmunoassay for serum, urine and cerebrospinal fluid

     

Radioimmunoassay for parathyroid hormone (PTH): Characterization of commercially available antigens and antisera

Ohne ZusammenfassungSupported by Deutsche Forschungsgemeinschaft.