ICP-AES测定耐冬山茶花中的矿质元素

用浓硝酸-高氯酸（4V∶1V）的混合溶液对耐冬花样品进行消解,采用电感耦合等离子体-原子发射光谱法（ICP-AES）测定了样品中Zn、Ca、Mn、Fe、Mg、B、和K7种矿质元素含量.各元素在实验范围内,线性关系良好,回收率在98％-103％,结果令人满意.     

长白山区野生金灯藤种子及种皮中总黄酮的含量测定

以芦丁为对照品,采用分光光度法测定了长白山区野生金灯藤成熟种子及种皮中总黄酮含量.芦丁在8.16-48.96μg/mL的范围内呈良好线性关系,测得种子及种皮中总黄酮含量分别为2.71％、0.91％,平均加标回收率为99.32％.该方法简单可行,灵敏度高,测定结果可靠,可用于测定金灯藤种子及种皮中总黄酮含量.     

RP-HPLC同时测定溪黄草药材中芦丁和槲皮素的含量

采用Kromasil C<,18>柱(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸水溶液(45:55,V/V),流速1.0mL/min,检测波长350nm,柱温25℃.结果表明:芦丁和槲皮素进样浓度分别在7.5-240.0μg/mL(r=0.9998)和5.0-160.0μg/mL(r=0.9995)范...     

STUDIES ON THE ISOLATION PURIFICATION AND CHARACTERIZATION OF THE FUCOIDAN-GALACTOSAN SULFATE (FGS) FROM LAMINARIA JAPONICA

1INTRODUCTIONFGSanditsderivationpossessremarkablemedicinalvalues:antitumouseffect,antiviralactivity,immunomodulation,bloodfatregulation,andhypoglycemia.Studiesonthepolysaccharidefromlaminariajaponicastartedatthebeginningof20thcentury.Thoughalginicacidfromlaminariajaponicahasalreadybeenindustrialized[1],peopledonotknownclearlyaboutlaminarinandfucoidan[2]becauseofrestrictionintheisolationandpurificationmethods[3].Weusedenzymolysistechnique,modernchromatographymethods,andspectralanalysistos…     

Abietane Quinones from Rabdosia serra

Rabdosiu Serra (M4-YId Hara is a chinese traditional medicine for the treatment ofhepatitis. enteritis. acute cholecystitis and dysentery'. The present communicationdescribes the first isolation of three abietane quinone diterpenoids from the herb (Figure1 ). The new abietane quinone, 16-acetoxy-7Q-ethoxyroyleanone I was elucidated. Inaddition. two known abietane quinones, horminone 2, 16-acetoxy-7-0-acetylhorminone 3and 6-sitosterol. stigmasterol. ursolic acid. palmitic acid were identifie…     

Simultaneous determination of phytochemicals and low-weight organic acids in Sophora japonica bud and premium noni juice through column-switching liquid chromatography connected with ion chromatography system

In this work, a liquid chromatography connected with an ion chromatography by the column-switching technique was developed for the simultaneous determination of three phytochemicals (scopoletin, rutin, and quercetin), three anions (chloride, sulfate, and phosphoric), and seven organic acids (lactic, acetic, propionic, formic, malic, oxalic, and citric acid) in Chinese traditional herbs, S. japonica bud and premium noni juice. A concentration column was used to collect organic acids and anions, and then the concentrated analytes were eluted to an analytical column by column-switching technique in which target analytes could be separated successfully and detected simultaneously within 70?min. This method showed good linearity with the linear correlation coefficients in the range of 0.9971–1.0000. The relative standard deviations of the peak areas were all less than 5.63% (n?=?6). The limits of detection (LOD, S/N?=?3) of anions and organic acids were in the range of 0.0006–0.0857?mg/L, whereas phytochemicals were in the range of 0.0123–0.0197?mg/L. Recoveries were in the range of 86.53–117.01% for the spiked sample. This method was successfully applied to the determination of the low-weight organic acids and phytochemicals in Chinese traditional herbal medicines, S. japonica bud and premium noni juice.     

Rearranged Calamenene and Eudesmane Sesquiterpenoids from two Chinese Liverworts

Four novel rearranged calamenene sesquiterpenoids, 1 – 4 , and two eudesmane sesquiterpenoids, 5 and 6 , were isolated from the Chinese liverworts Chiloscyphus polyanthus (L.) and Bazzania japonica S. (Lac .) Lindb . Their structures and relative configurations were determined by chemical derivatization and in‐depth spectroscopic methods, especially 1D‐ and 2D‐NMR as well as HR‐MS analyses.     

三种西藏香茶菜挥发油化学成分的研究

唇形科植物香茶菜广泛分布于全国各地,民间一直用作抗菌消炎药使用,近年来发现,香茶菜属植物有抑制肿瘤和抗癌活性,其活性成分是二萜。我们对采自西藏的3种香茶菜的挥发油进行了GC/MS分析。从小叶香茶菜[Rabdosia Parrifolia(Batal)Hara; Litte leaf Rabdosia]油中分出84个峰,鉴定了27个,占总面积的60％;从川藏香茶菜(Rabbosia Pseudo-irrarata C.Y.Wu,Szechwan-Tibet Rabdosia)油中分出72个峰,鉴定了39个,占总面积的82.5％;从皱叶香茶菜[Rabdosia rugosa(Wcll.)Hara,Wrinkted leaf Rabdosia]中分出了90个峰,鉴定了39个,占总面积的62.1％。3种香茶菜的分析尚未见报道。     

Physicochemical Properties and Anticoagulant Activity of Purified Heteropolysaccharides from Laminaria japonica

Laminaria japonica is widely consumed as a key food and medicine. Polysaccharides are one of the most plentiful constituents of this marine plant. In this study, several polysaccharide fractions with different charge numbers were obtained. Their physicochemical properties and anticoagulant activities were determined by chemical and instrumental methods. The chemical analysis showed that Laminaria japonica polysaccharides (LJPs) and the purified fractions LJP0, LJP04, LJP06, and LJP08 mainly consisted of mannose, glucuronic acid, galactose, and fucose in different mole ratios. LJP04 and LJP06 also contained minor amounts of xylose. The polysaccharide fractions eluted by higher concentration of NaCl solutions showed higher contents of uronic acid and sulfate group. Biological activity assays showed that LJPs LJP06 and LJP08 could obviously prolong the activated partial thromboplastin time (APTT), indicating that they had strong anticoagulant activity. Furthermore, we found that LJP06 exerted this activity by inhibiting intrinsic factor Xase with higher selectivity than other fractions, which may have negligible bleeding risk. The sulfate group may play an important role in the anticoagulant activity. In addition, the carboxyl group and surface morphology of these fractions may affect their anticoagulant activities. The results provide information for applications of L. japonica polysaccharides, especially LJP06 as anticoagulants in functional foods and therapeutic agents.     

Ligand fishing of anti‐neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B

In this work, monoamine oxidase B was immobilised onto magnetic nanoparticles to prepare a new type of affinity solid‐phase extraction adsorbent, which was used to extract the possible anti‐neurodegenerative components from the Lonicera japonica flower extracts. Coupled with high‐performance liquid chromatography with mass spectrometry, two monoamine oxidase B ligands were fished‐out and identified as isochlorogenic acid A and isochlorogenic acid C, which were found to be inhibitors of the enzyme for the first time, with similar half maximal inhibitory concentration values of 29.05 ± 0.49 and 29.77 ± 1.03 μM, respectively. Furthermore, equilibrium‐dialysis dissociation assay of enzyme‐inhibitor complex showed that both compounds have reversible binding patterns to monoamine oxidase B, and kinetic analysis demonstrated that they were mixed‐type inhibitors for monoamine oxidase B, with K_i and K_is values of 9.55 and 37.24 μM for isochlorogenic acid A, 9.53 and 35.50 μM for isochlorogenic acid C, respectively. The results indicated that isochlorogenic acid A and isochlorogenic acid C were the major active components responsible for the anti‐degenerative activity of the flowers of L. japonica, while magnetic nanoparticles immobilised monoamine oxidase B could serve as an efficient solid‐phase extraction adsorbent to specifically extract monoamine oxidase B inhibitors from complex herbal extracts.