On-line coupling of counter-current chromatography and macroporous resin chromatography for continuous isolation of arctiin from the fruit of Arctium lappa L.

In this work, we have developed a novel hybrid two-dimensional counter-current chromatography and liquid chromatography (2D CCC × LC) system for the continuous purification of arctiin from crude extract of Arctium lappa. The first dimensional CCC column has been designed to fractionalize crude complex extract into pure arctiin effluent using a one-component organic/salt-containing system, and the second dimensional LC column has been packed with macroporous resin for on-line adsorption, desalination and desorption of arctiin which was effluent purified from the first CCC dimension. Thus, the crude arctiin mixture has been purified efficiently and conveniently by on-line CCC × LC in spite of the use of a salt-containing solvent system in CCC separation. As a result, high purity (more than 97%) of arctiin has been isolated by repeated injections both using the ethyl acetate–8% sodium chloride aqueous solution and butanol–1% sodium chloride aqueous solution. By contrast with the traditional CCC processes using multi-component organic/aqueous solvent systems, the present on-line CCC × LC process only used a one-component organic solvent and thus the solvent is easier to recover and regenerate. All of used solvents such as ethyl acetate, n-butanol and NaCl aqueous solution are low toxicity and environment-friendly. Moreover, the lower phase of salt-containing aqueous solution used as mobile phase, only contained minor organic solvent, which will save much organic solvent in continuous separation. In summary, our results indicated that the on-line hybrid 2D CCC × LC system using one-component organic/salt-containing aqueous solution is very promising and powerful tool for high-throughput purification of arctiin from fruits of A. lappa.     

Accelerated Transfer and Spillover of Carbon Monoxide through Tandem Catalysis for Kinetics-boosted Ethylene Electrosynthesis

Cu-based catalysts have been widely applied in electroreduction of carbon dioxide (CO₂ER) to produce multicarbon (C₂₊) feedstocks (e.g., C₂H₄). However, the high energy barriers for CO₂ activation on the Cu surface is a challenge for a high catalytic efficiency and product selectivity. Herein, we developed an in situ *CO generation and spillover strategy by engineering single Ni atoms on a pyridinic N-enriched carbon support with a sodalite (SOD) topology (Ni-SOD/NC) that acted as a donor to feed adjacent Cu nanoparticles (NPs) with *CO intermediate. As a result, a high C₂H₄ selectivity of 62.5 % and an industrial-level current density of 160 mA cm⁻² at a low potential of −0.72 V were achieved. Our studies revealed that the isolated NiN₃ active sites with adjacent pyridinic N species facilitated the *CO desorption and the massive *CO intermediate released from Ni-SOD/NC then overflowed to Cu NPs surface to enrich the *CO coverage for improving the selectivity of CO₂ER to C₂H₄.     

Analgesic Buxus alkaloids with Enhanced Selectivity for the Low-Voltage-Gated Calcium Channel Cav3.2 over Cav3.1 through a New Binding Mode

Low-voltage-gated calcium channels (LVGCCs; Ca_v3.1-3.3) represent promising drug targets for epilepsy, pain, and essential tremor. At present, modulators with heightened selectivity for a subtype of LVGCCs are still highly desired. In this study we explored three classes of Buxus alkaloids and identified 9(10/19)abeo-artanes Buxusemine H and Buxusemine L (BXSL) as an unprecedented type of Ca_v3.2 inhibitors. Particularly, BXSL exhibited Ca_v3.2 inhibition comparable to Z944, a non-subtype-selective LVGCCs inhibitor under clinical trial. While lacking specificity for Ca_v3.3, BXSL showed a 30-fold selectivity of Ca_v3.2 over Ca_v3.1. As compared to several well-known inhibitors, the experimental and computational studies suggested BXSL exhibits a distinct binding mode to Ca_v3.2, notably through the essential interaction with serine-1543 in domain III. Furthermore, BXSL showed minimal impact on various recombinant and native nociceptive ion channels, while significantly reducing the excitability of isolated mouse dorsal root ganglion neurons. Animal studies in wild-type and Ca_v3.2 knock-out mice revealed that BXSL (5 mg/kg), by inhibiting Ca_v3.2, exhibits an analgesic effect equivalent to Z944 (10 mg/kg) or mibefradil (10 mg/kg). Moreover, we proposed a structural rationale for the high selectivity of 9(10/19)abeo-artane-type alkaloids towards Ca_v3.2 over Ca_v3.1. This study introduces a novel analgesic agent and valuable molecular insight for structure-based innovative Ca_v3.2 drug development.     

DAD检测干鱿鱼制品中苯甲酸、山梨酸的含量

通过乙醚提取样品,在C18色谱柱上(4.6×250mm,5μm)以磷酸缓冲溶液和甲醇以95:5的比例作为流动相进行色谱分离,用HPLC法DAD 225nm处检测干鱿鱼制品中的苯甲酸、山梨酸含量.本法在标准浓度为0.01-0.1mg/mL范围内,线性良好,相关系数大于0.995,该方法适合日常检测.     

I.I.D.随机变量两两乘积之和的Hsu-Robbins型定理(Ⅱ)

在本部分中,采用由 Ｌｅｖｙ函数（４．ｌ）所确定的值ｂｎ取代（Ｉ）［１］中的１／ｎ“位点”ａｎ,使得（Ｉ）中定理１中的附加条件（Ａ）得以解除,从而获得了可以称之为Ｈｓｕ－Ｒｏｂｂｉｎｓ型的充分必要条件．作为推论,还给出了两个互为复制的ｉｉｄ随机变量三角阵对应行之和乘积的Ｋｏｌｍｏｇｏｒｏｖ强大数律．     

A new water treatment technology for degradation of B[a]A by hydrodynamic cavitation and chlorine dioxide oxidation

Managing environmental contamination with Benz[a]anthracene (B[a]A) is essential due to its carcinogenic, teratogenic and mutagenic effects on humans and the environment. At present, the mainly B[a]A degradation methods used are photodegradation, bioremediation and traditional advanced oxidation, although they all have disadvantages. In this study, B[a]A was degraded by hydrodynamic cavitation (HC), chlorine dioxide (ClO₂), or an innovative combination of the two methods. According to high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC–MS) analysed the degradation products and degradation pathway of B[a]A, with the kinetics of different degradation methods discussed. Under optimal conditions, HC combined with ClO₂ oxidation can further degrade products to achieve ring cleavage. Compared with the two separate degradation process methods, the combined method exerts a synergistic effect on the degradation of B[a]A, with an enhancement factor of 1.48. Experimental results showed that the combination method can realize enhanced complete degradation of B[a]A, reduce ClO₂ requirements, improve efficiency, reduce energy consumption and produce less harmful products with ring cleavage achieved.     

2n阶微分算子乘积自伴的充分必要条件

给定Hilbert空间L2[a,∞)上两个由2n阶对称微分算式生成的微分算子Li(i=1,2),该文给出了乘积算子L2L1是自伴算子的一个充分必要条件．     

Squares of characters and finite groups

Let G be a finite group and χ be an irreducible complex character. We study the character χ² in the case that χ(1) is a prime power.     

Stable Laws and Products of Positive Random Matrices

Let S be the multiplicative semigroup of q×q matrices with positive entries such that every row and every column contains a strictly positive element. Denote by (X _n ) _n≥1 a sequence of independent identically distributed random variables in S and by X ⁽ⁿ⁾=X _n ⋅⋅⋅ X ₁,  n≥1, the associated left random walk on S. We assume that (X _n ) _n≥1 satisfies the contraction property