A New Furospirostanol Saponin, Ophiofurospiside B from Ophiopogon japonicus（ Thunb. ） Ker-Gawl

Introduction The tuber ofOphiopogon japonicus(L.f)KerGawl,which is a Chinese traditional medicine named "maidong"have been recorded to have various medical functions for curing cardiovascular diseases and bacterial infections,especially heart diseases.Phytochemical studies on this plant were reported previously[1].     

Two New Diterpenoids and Other Constituents from Isodon japonicus

Two new ent‐kaurene diterpenoids, 15α,20‐dihydroxy‐6,7‐seco‐entkaur‐16‐ene‐7,1α(6,11α)‐diolide ( 1 ), 6β‐butyroxy‐3β‐hydroxy‐6,7‐seco‐6,20‐epoxy‐7,1α‐olide‐entkaur‐16‐en‐15‐one ( 2 ), together with 25 known compounds, 3 – 27 , were isolated from the leaves of Isodon japonicus. Their structures were established by spectroscopic methods, including 2D‐NMR techniques.     

An efficient combination of supercritical fluid extraction and high‐speed counter‐current chromatography to extract and purify homoisoflavonoids from Ophiopogon japonicus (Thunb.) Ker‐Gawler

Supercritical fluid extraction (SFE) was used to extract homoisoflavonoids from Ophiopogon japonicus (Thunb.) Ker‐Gawler. The optimization of parameters was carried out using an orthogonal test L₉ (3)⁴ including pressure, temperature, dynamic extraction time and the amount of modifier. The process was then scaled up by 100 times with a preparative SFE system under the optimized conditions of 25 MPa, 55°C, 4.0 h and 25% methanol as a modifier. Then crude extracts were separated and purified by high‐speed counter‐current chromatography (HSCCC) with a two‐phase solvent system composed of n‐hexane/ethyl acetate/methanol/ACN/water (1.8:1.0:1.0:1.2:1.0 v/v). There three homoisoflavonoidal compounds including methylophiopogonanone A 6‐aldehydo‐isoophiopogonone A, and 6‐formyl‐isoophiopogonanone A, were successfully isolated and purified in one step. The collected fractions were analyzed by HPLC. In each operation, 140 mg crude extracts was separated and yielded 15.3 mg of methylophiopogonanone A (96.9% purity), 4.1 mg of 6‐aldehydo‐isoophiopogonone A (98.3% purity) and 13.5 mg of 6‐formyl‐isoophiopogonanone A (97.3% purity) respectively. The chemical structure of the three homoisoflavonoids are identified by means of ESI‐MS and NMR analysis.     

Two new sesquiterpenes from Chloranthus japonicus Sieb

Two new sesquiterpenes, namely, 1β,10β-dihydroxy-eremophil-7(11), 8-dien-12,8-olide (1) and 8,12-epoxy-1β-hydroxyeudesm-3,7,11-trien-9-one (2), together with three known sesquiterpenoids, shizukolidol (3), 4α-hydroxy-5α(H)-8β-methoxy-eudesm-7(11)-en-12,8-olide (4), and neolitacumone B (5), and two known monoterpenes, (3R,4S,6R)-p-menth-1-en-3,6-diol (6) and (R)-p-menth-1-en-4,7-diol (7), were isolated from the whole plant of Chloranthus japonicus Sieb. Their structures were elucidated on the basis of spectroscopic data analysis and comparison with those of related known compounds. Compounds 4–7 were isolated from this plant for the first time.     

日本续断中新五糖常春藤皂苷的结构和NMR研究

从日本续断根部的乙醇提取物中分得一个新的三萜皂苷.经过测定,它为:3-O-[β-D-吡喃葡萄糖(1→4)][α-L-吡喃鼠李糖(1→3)]-β-D-吡喃葡萄糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖常春藤甙元.结果表明,采用一维SEMDY和旋转坐标NOE差谱核磁共振新技术相结合的方法测定这一类化合物的糖链结构不需要对化合物进行化学降解或衍生化,方法简便、快速,测定结果可靠,每个糖基的¹H NMR信号可以分辨和明确归属.     

日本续断中新的双糖链三萜皂苷的结构研究

从日本续断根部的乙醇提取物中分得一个新的三萜皂苷。经过测定,它为：3-O-α-L-吡喃鼠李糖(1→3)-β-D-吡喃葡萄糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖9常春藤苷元－28-O-β-吡喃葡萄糖酯苷)。结果表明,采用一维SEMDY,旋转坐标NOE差谱和选择性远程DEPT核磁共振新技术相结合的方法测定糖链结构不需要对化合物进行化学降解或衍生化,方法简便、快速,测定结果可靠,每个糖基的信号可以分辨和明确归属。     

刺参中无机元素的聚类分析和主成分分析

探讨多元素分析对中国刺参产地溯源的可行性,筛选判别刺参产地的有效指标.采用电感耦合等离子体质谱(ICP-MS)法测定了7个产地刺参样品中Al,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,As,Se,Mo,Cd,Hg和Pb共15种无机元素的含量,建立刺参无机元素数据库.原始数据经过标准化处理后,结合聚类分析和主成分分析...     

在滞尘影响下的植被叶片光谱变化特征研究

为建立以高光谱数据为基础的叶片滞尘质量反演模型,沿北京市区采集了30个大叶黄杨叶片样本。利用电子分析天平和光谱仪(analytical spectral devices ASD FieldSpec Pro)测定“除尘前”与“除尘后”叶片质量及光谱反射率曲线,以获取叶片尘埃量、光谱信息等数据。随后以传统意义和偏最小二乘(PLS)回归模型为基础,以探究空气尘埃量与光谱曲线之间可能存在的关系,阐述了叶片尺度上尘埃量对植物光谱特征的影响。结果为：除尘前后叶片光谱曲线在350～700, 780～1 300, 1 900～2 500 nm波段区间内有较大差异,同时尘埃量与叶片单波段光谱反射率比值呈负相关,相关度最大值点为737波段,属于近红外波段,相关系数可达-0.8左右。在尘埃量与叶片光谱多波段组合关系研究中得到,948和945波段构成的NDVI指数与尘埃量的相关度最大,相关系数可达0.76。在叶片滞尘量反演研究中,对比传统意义滞尘量回归模型,引进的偏最小二乘算法(PLS)可使叶片滞尘量反演精度略有提高,最后由回归模型精度评定可得偏最小二乘法反演效果较传统回归好。     

A new triterpene saponin from Panax japonicus C. A. Meyer var major (Burk.) C. Y. Wu et K. M. Feng

<正>One new triterpene saponin was isolated from Panaxjaponicus C.A.Meyer var major(Burk.) C.Y.Wu et K.M.Feng,and established as oleanolic acid 3-O-[β-D-glucopyranosyl-(1→2)-β-D-glucuronopyranosyl-6'-O-n-butyl ester]which showed moderate antitumor activities against the A2780 cells and OVCAR-3 cells.Its structure was established by means of spectral data, particularly NMR,including HSQC and HMBC techniques.     

Three minor new compounds from the aerial parts of Leonurus japonicus

Phytochemical investigation of the aerial parts of Leonurus japonicus led to the isolation of one new labdane diterpenoid, leojaponin D(1) and two new ionone derivatives, leojaponones A and B(2 and 3),together with seven known diterpenoids(4–10). Their structures were elucidated by extensive 1D and2 D NMR spectroscopic data and by comparison with data reported in the literature. Selected isolates were evaluated their effects on Jurkat IL2 secretion.