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31.
Ninhydrin reaction on thiol-reactive solid and its potential for the quantitation of d-penicillamine
While aminothiols produce weak purple-colored reactions with ninhydrin, we demonstrate for the first time that this color could be intensely developed. Using a d-penicillamine paradigm, adsorption of this compound via a disulfide bond onto thiol-reactive solid prior to ninhydrin reaction allowed spectrophotometrical monitoring of the supernatant at 570 nm. Compared with off-solid method, this approach expanded the linear concentration range to 50-600 μg mL−1 and enhanced the sensitivity so that d-penicillamine with the concentrations of less than 100 μg mL−1 could be accurately quantitated by using a second-order polynomial calibration curve. Additionally, this assay was unaffected by disulfide adduct interference, highlighting its potential for the analysis of d-penicillamine as well as other aminothiols. 相似文献
32.
Yan Cao Afrasyab Khan Hanzaleh Balakheyli Andrew Ng Kay Lup Mohammad Ramezani Taghartapeh Hassan Mirzaei Seyed Reza Khandoozi Alireza Soltani Mehrdad Aghaei Fatemeh Heidari Shaheen M. Sarkar Ahmad B. Albadarin 《Arabian Journal of Chemistry》2021,14(7):103200
The adsorption of penicillamine (PCA) on pure B12N12 and B12CaN12 nanocages in aqueous and chloroform solvents has been evaluated using density functional theory (DFT) calculations. The interaction of PCA on B12N12 nanocages is chemisorption through its four nucleophilic sites: amine, carbonyl, hydroxyl and thiol. The most stable adsorption configuration was achieved when zwitterionic PCA adsorbs via its carbonyl group in water with value of ?1.723 eV, in contrast, when neutral PCA adsorbs via its amine group in chloroform with value of ?1.68 eV. Intercalated calcium ion within B12N12 nanocage (B12CaN12) was shown to attract PCA onto nanocage surface, resulting in higher solubility and adsorption energy after their complexation in water and chloroform. The adsorption of multiple PCA molecules from their amine and carbonyl groups on pure and B12CaN12 nanocages were also evaluated where two and three molecules can be chemisorbed on boron atoms of the nanocage surfaces with the adsorption energy per PCA reduces slightly with the increasing the amount of drugs due to the curvature effects. Molecular docking study indicates that PCA from its NH2 group on B12CaN12 nanocage has the best binding affinity and inhibition potential of tumor necrosis factor-alpha (TNF-α) and Interleukin-1 (IL-1) receptors as compared with the other adsorption systems. Molecular docking and ADMET analysis displayed that the chosen compounds pass Lipinski Rule and have appropriate pharmacokinetic features suitable as models for developing anti-inflammatory agents. 相似文献