多勒普加宽斯塔克效应调制分析

用半经典理论分析了含多勒普加宽的斯塔克效应调制，使用密度矩阵近似，导出了吸收线附近ＮＨ３分子的吸收系数和调制度表达式，实验研究了１０．７８４μｍ波长的斯塔克效应调制特性，调制频率１ＭＨｚ时，调制度达到３３％。     

Reply by Liu et al.

The experimental results indicate that the composition of our samples is homogeneous. We have explained the reason why the composition of the BCN film is influenced by the r.f. power. The Fourier transformed infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) measurements suggest that the samples are atomic-level hybrids of B, C and N.     

受驱无序胶体动力学

利用Langevin分子动力学，本数值研究点钉扎中心随机分布的二维胶体动力学．随着钉扎中心强度的提高，我们发现了从弹性脱钉到塑性脱钉的渡越，并伴随临界钉扎力在渡越区的明显提高，类似于超导体中的峰值效应．另外，我们首次发现：当塑性流动发生时，高速运动胶体粒子感受到的平均钉扎力在从玻璃态到液态的转变过程中会出现峰值效应，并伴随有速度-驱动力曲线的交叠．     

二维卷积及其在色谱峰纯度鉴定中的应用

光电二极管阵列检测器与色谱仪的联用 ( HPLC- DAD)为复杂体系中多组分的定性定量分析提供了可能 .Savitzky等 [1]提出了以简单的卷积运算完成数值微分方法 .Gorry等 [2 ]曾以 Gram正交多项式替代幂级数拟合 ,让卷积技术可以覆盖到谱图包括起讫部分的任一点 .Edwards[3]与 Kuo[4 ]等提出的二维卷积法对处理和利用二变量数据矩阵具有普遍意义 ,但理论推导繁琐 .我们曾完善了推求二维卷积整数的普遍方法 [5] ,并研究和解决了一维和二维卷积中的信息丢失问题 [6 ,7] .本文提出了一种简化的求算二维卷积整数的方法 .理论推导表明 ,二维卷积…     

胰岛素反相制备色谱的方法开发

以胰岛素反相制备色谱方法的开发和优化为目标,通过考察色谱保留参数、峰展宽及样品流出曲线的浓度分布等色谱参数,对流动相梯度、色谱填料、载样量等色谱条件进行了优化,并建立了胰岛素制备色谱峰参数的描述方法。结果表明,所建立的方法可快速筛选出最适于胰岛素分离的色谱条件(包括流动相梯度及分离填料),即流动相中的强洗脱溶剂(有机相)需采取缓梯度窄区间的变化条件,筛选出的分离填料需具备峰向两侧展宽且展宽程度较小、样品最高浓度居中分布的特点。将方法用于实际胰岛素粗品的纯化制备,获得了杂质去除效果好、胰岛素纯度高的产品。该法为胰岛素反相色谱纯化制备方法的快速建立提供了指导,具有较强的实用价值,同时为发展大分子化合物的制备色谱方法提供了参考。     

Electron broadening of isolated lines with stationary non-equilibrium level populations

It is shown that a quantum kinetic theory approach to line broadening, extended to stationary non-equilibrium states, yields corrections to the standard electron impact widths of isolated lines that depend on the population of the radiator internal levels. A consistent classical limit from a general quantum treatment of the perturbing electrons also introduces corrections to the isolated line widths. Both effects are essential in preserving detailed-balance relations. Preliminary analysis indicates that these corrections may resolve existing discrepancies between theoretical and experimental widths of isolated lines. An experimental test of the results is proposed.     

Wavelength-dispersive spectral analysis and quantification of monazites by electron-probe microanalysis

The problem of determining the chemical composition of monazite grains through electron probe microanalysis is studied, by using a scanning electron microscope with a wavelength dispersive spectrometer. A careful qualitative analysis is performed with the purpose of determining all the elements present in the samples, the lines to be used in the quantifications trying to minimize interferences, the angular positions and the acquisition times for the measurement of peak and background intensities and the crystals to be used. Particular emphasis is devoted to the analysis of Th, U and Pb, which are used to determine the age of the rock by means of the U-Th-Pb method, commonly used in geochronology. Quantitative determinations of the chemical composition of monazite grains are performed, optimizing the experimental conditions on the basis of the qualitative analysis. The determinations were made under two different criteria of quantification of oxygen, and the dissimilar results obtained are discussed.     

Characterization of interaction kinetics between chiral solutes and human serum albumin by using high-performance affinity chromatography and peak profiling

Peak profiling and high-performance columns containing immobilized human serum albumin (HSA) were used to study the interaction kinetics of chiral solutes with this protein. This approach was tested using the phenytoin metabolites 5-(3-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) and 5-(4-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) as model analytes. HSA columns provided some resolution of the enantiomers for each phenytoin metabolite, which made it possible to simultaneously conduct kinetic studies on each chiral form. The dissociation rate constants for these interactions were determined by using both the single flow rate and multiple flow rate peak profiling methods. Corrections for non-specific interactions with the support were also considered. The final estimates obtained at pH 7.4 and 37°C for the dissociation rate constants of these interactions were 8.2-9.6 s(-1) for the two enantiomers of m-HPPH and 3.2-4.1 s(-1) for the enantiomers of p-HPPH. These rate constants agreed with previous values that have been reported for other drugs and solutes that have similar affinities and binding regions on HSA. The approach used in this report was not limited to phenytoin metabolites or HSA but could be applied to a variety of other chiral solutes and proteins. This method could also be adopted for use in the rapid screening of drug-protein interactions.