抚顺烟煤吡啶抽出物ZM2馏份结构表征

本文采用了多种现代结构分析方法(FTIR,~1H-、~(13)C-NMR,FDMS,MS/MS)对抚顺烟煤吡啶抽出物的正庚烷不溶、正庚烷/苯(1∶1)可溶馏份(ZM2馏份)进行了详细的结构解析,得到了其平均分子量、分子量分布及其中30种化合物的分子结构,为研究抚顺烟煤的结构提供了有价值的信息。     

Anti-inflammatory activity of 4′,6,7-trihydroxy-5-methoxyflavone from Fridericia chica (Bonpl.) L.G.Lohmann

Abstract

Fridericia chica, Bignoniaceae, is a tropical tree-creeper used as a traditional remedy for a number of diseases, highlighting inflammation. Our objective was to corroborate the popular anti-inflammatory use of the hydroethanolic extract from the leaves (HEFc) and of its isolated 4′,6,7-trihydroxy-5-methoxyflavone (5-O-methylscutellarein) [1], described here for the first time. Quantitative analysis indicated 8.77?±?0.23?mg/g of this compound in the extract. Neither HEFc nor [1] was cytotoxic in vitro. In LPS-induced peritonitis in mice, oral pre-treatment with HEFc or [1] led to decreased leukocyte migration to the peritoneal cavity and a reduction in the concentrations of pro-inflammatory cytokines (TNFα and IL-1β). Also, the anti-inflammatory cytokine IL-10 was enhanced following treatment with [1]. Overall, these results validate the traditional use of Fridericia chica as anti-inflammatory, and indicate that the compound 5-O-methylscutellarein may participate in this effect.     

High performance liquid chromatography with electrochemical detection. 1: Separation of Cannabis constituents and quantitation of Δ9 tetrahydrocannabinol

A simple, isocratic high performance liquid chromatography system (HPLC) with electrochemical detection (EC) was used to study Cannabis constituents. Several Cannabis extracts and reference standards were examined. A total of eleven constituents were separated, and three major cannabinoids; Δ⁸, Δ⁹ tetrahydrocannabinol (THC) and cannabidiol (CBD) were identified. A linear relationship was established for the quantitation of the halucinogenic constituent Δ⁹ THC. Minor contaminants in Δ⁹ THC reference standard, which were not detected by gas chromatography (GC) were detected for the first time. The detection limits of Δ⁹ THC and related cannabinoids were in the low nanogram range (2–20 ng).     

两种陆生植物浸提液对蛋白核小球藻(Chlorella pyrenoidosa)的化感作用

通过合果芋(Syngonium podophyllum)和榕树(Ficus microcarpa)两种陆生植物不同浓度、不同部位浸提液对蛋白核小球藻化感作用的研究,结果表明:两种植物对蛋白核小球藻都具有化感作用但强度不同,榕树的抑制效果比合果芋强且稳定.从化感作用的效果上看,合果芋浸提液对蛋白核小球藻呈现先抑制后促进的作用特性,而榕树浸提液始终具有抑制作用.除榕树气生根浸提液外,在抑制作用期间内不同浓度浸提液对蛋白核小球藻的化感作用强度大致随浸提液浓度的增大而增强.不同陆生植物或同株植物不同部位浸提液的化感作用强度也有所不同,一般榕树叶(EC50,96h为13.16g·L-1)合果芋叶(EC50,96h为14.37g·L-1)榕树气生根(EC50,96h为30.66g·L-1).     

Aroma Properties of Cocoa Fruit Pulp from Different Origins

Cocoa pulp occurs as a by-product of cocoa bean production and can be repurposed to different food applications, such as jams, fruit preparations and beverages, improving the sustainability of cocoa production, as well as the livelihoods of cocoa farmers. In this work, aroma-active compounds of fresh cocoa fruit pulps from different origins were investigated by applying aroma extract dilution analyses in combination with gas chromatography-mass spectrometry/olfactometry for identification. In total, 65 aroma-active compounds were determined in four different pulps originating from Indonesia, Vietnam, Cameroon, and Nicaragua. Vietnamese pulp showed the highest number of aroma-active regions, while Cameroonian pulp accounted for the lowest. Moreover, Cameroonian cocoa pulp showed the lowest FD factors. Overall, the odorants with the highest FD factors were trans-4,5-epoxy-(E)-decenal, 2- and 3-methylbutanoic acid, 3-(methylthio)propanal, 2-isobutyl-3-methoxypyrazine, (E,E)-2,4-nonadienal, (E,E)-2,4-decadienal, 4-vinyl-2-methoxyphenol, δ-decalactone, 3-hydroxy-4,5-dimethylfuran-2(5H)-one, dodecanoic acid, and linalool. This study provides insights into the aroma composition of fresh cocoa pulp from different origins for future food applications.     

Bioactive Pectin-Murta (Ugni molinae T.) Seed Extract Films Reinforced with Chitin Fibers

This study investigated the biocomposite pectin films enriched with murta (Ugni molinae T.) seed polyphenolic extract and reinforced by chitin nanofiber. The structural, morphological, mechanical, barrier, colorimetric, and antioxidant activity of films were evaluated. The obtained data clearly demonstrated that the addition of murta seed extract and the high load of chitin nanofibers (50%) provided more cohesive and dense morphology of films and improved the mechanical resistance and water vapor barrier in comparison to the control pectin film. The antioxidant activity ranged between 71% and 86%, depending on the film formulation and concentration of chitin nanofibers. The presented results highlight the potential use of chitin nanofibers and murta seed extract in the pectin matrix to be applied in functional food coatings and packaging, as a sustainable solution.     

Inhibition of Interleukin-6-Induced Matrix Metalloproteinase-2 Expression and Invasive Ability of Lemon Peel Polyphenol Extract in Human Primary Colon Cancer Cells

Among matrix metalloproteinases (MMPs), MMP-9/2 are key enzymes involved in the proteolysis of extracellular matrices in the inflammatory process and in cancer. Since MMP-9/2 expression levels, activity, and secretion is up-regulated during inflammation in response to pro-inflammatory cytokines, such as interleukin-6 (IL-6), many efforts have been devoted to identifying factors that could inhibit the IL-6-induced MMP-9/2 expression. Up to now, several reports indicated that polyphenols from fruits and vegetables are among the major components of health promotion for their antioxidant properties and also for their anti-inflammatory and anti-cancer agents. Among plant derived polyphenols, lemon (Citrus limon) peel extract (LPE) shows anti-cancer properties in various cancer types. In our previous work, we demonstrated that LPE can reduce IL-6-induced migration/invasiveness and MMP-9/2 up-regulation in some gastric cancer cell lines. This study aims to exploit the anti-cancer properties of LPE using an in vitro system model of inflammation, consisting of IL-6-exposed human primary colon cancer cells. We first analyzed the effect of LPE on IL-6-induced cell migration and invasiveness by wound healing and Boyden chamber assay, respectively. The MMP-2 mRNA expression levels and gelatinolytic activity in the cell culture media were determined by q-PCR analysis and gelatin zymography, respectively, and finally, the effects of LPE on IL-6-induced JAK2/STAT3 signaling pathways have been investigated by Western blotting analysis. Our results show that LPE is able to inhibit the IL-6-dependent cell migration and invasiveness associated with the up-regulation of MMP-2 expression levels and that these effects are correlated to the STAT3 phosphorylation in human primary T88 and T93 colon cancer cells.     

Isolation and Characterization of a Bioactive Polysaccharide from Panax Quinquefolium L.

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导数分光光度法测定毒饵中的敌鼠钠

用紫外二阶导数分光光度法测定毒饵中敌鼠钠含量。当样品中含有0.4g·L-1、2g·L-1敌鼠钠时平均回收率为96.8%。标准曲线范围为1～5mg·L-1,相关系数为0.9998,相对标准偏差为0.6%～2.2%,检出限为0.15mg·L-1。