Role of Time Scales in the Coupled Epidemic-Opinion Dynamics on Multiplex Networks

Modelling the epidemic’s spread on multiplex networks, considering complex human behaviours, has recently gained the attention of many scientists. In this work, we study the interplay between epidemic spreading and opinion dynamics on multiplex networks. An agent in the epidemic layer could remain in one of five distinct states, resulting in the SIRQD model. The agent’s attitude towards respecting the restrictions of the pandemic plays a crucial role in its prevalence. In our model, the agent’s point of view could be altered by either conformism mechanism, social pressure, or independent actions. As the underlying opinion model, we leverage the q-voter model. The entire system constitutes a coupled opinion–dynamic model where two distinct processes occur. The question arises of how to properly align these dynamics, i.e., whether they should possess equal or disparate timescales. This paper highlights the impact of different timescales of opinion dynamics on epidemic spreading, focusing on the time and the infection’s peak.     

大鼠去势后肾上腺与前列腺中Cyp17a1基因的表达

通过定量检测大鼠去势前后肾上腺和前列腺中Cyp17a1基因的表达, 试图从分子水平解释去势手术后大鼠机体内雄性激素水平的变化.     

正常人和结直肠癌患者血清的拉曼光谱探测与区别

本文对比分析了正常人和结直肠癌患者的血清的拉曼光谱.选择拉曼峰信息和荧光背景信息作为参数进行统计分析.用主成分回归(Principal component regression,PCR)和线性辨别分析(linear discriminant analysis,LDA)对所选参数进行分析以观察这些参数对光谱的代表性,得到...     

密集型波分复用薄膜干涉滤光片的设计

介绍了波分复用系统对薄膜干涉滤光片的基本要求，为了满足这些要求，一方面需要精心选择基板和薄膜材料，另一方面要寻找性能优良，制造容易的膜系，文中提出了二种适宜于设计这种滤光片的方法。     

烟叶中六种成分OSC-PCR定量模型的研究

采用近红外光谱技术对烟草中常规化学成分总糖、 还原糖、 烟碱、 总氮、 淀粉和挥发性碱进行测定。利用正交信号校正法(OSC)对烟叶的近红外光谱进行预处理,再使用主成分回归方法(PCR)建立烟叶中六种化学成分的定量分析模型,采用蒙特卡洛交互验证作为集成的建模策略优化模型参数,使用外部预测的相对预测性能(RPD)评价模型。结果表明,OSC有效解决了PCR投影方向并非浓度相关性最大方向的问题,同时解决了噪声、 基线漂移、 杂散光等问题。OSC-PCR建立的模型能够有效检测烟草常规化学成分。该研究方法通过确定化学值的波动范围初步监控烟叶中常规成分的含量,对于烟草品质评价和控制质量稳定性以及烟草香气成分分析具有重要意义。     

Quantitative Detection of PremicroRNA-21 Based on Chimeric Molecular Beacon

As an important tumor marker, the expression level of premicroRNA-21 (pre-miR-21) can provide important information for early diagnosis, drug treatment and prognosis of tumor. Thus, developing pre-miR-21 detection is of great importance for diagnosis of disease. Herein, a direct, simple and rapid method for quantitative detection of pre-miR-21 was developed. This detecting system contained the tool of molecular beacon which could hybridize specifically with pre-miR-21 and form duplex to induce fluorescence signal change. When loop bases hybridized with the pre-miR-21 in the solution to form a double-stranded duplex, the fluorophore and the quencher was separated and caused fluorescence recovery. We developed the new method for pre-miR-21 assay with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the level of pre-miR-21 in different tumor cells and tissues. The results showed that pre-miR-21 level was tightly related with tumor cells origins and malignancy degree. In 16 cases of gastric cancer tissues and adjacent tissues, the level of pre-miR-21 in 12 cases of cancer tissues was higher than that of adjacent tissues; 3 cases had lower expression level than that of adjacent tissues, and 1 case had no significant difference. Furthermore, Quantitative real-time PCR (qRT-PCR) method was used in to confirm the reliability of the detection results. The consistent results of the two different methods clearly showed that the molecular beacon assay with advantages of simplicity and rapidity for pre-miR-21 detection was hopeful for the wide usability in the function research and clinical diagnosis of pre-microRNA.     

Multiplexed time-correlated single-photon counting

We review the technique of multiplexed time-correlated single-photon counting whereby multiple fluorescence decay curves are recorded in parallel by statistically time-sharing a single time-to-amplitude converter. Application of the multiplexing technique to measuring the fluorescence lifetime topography of a self-absorbing sample is demonstrated. Further possibilities are discussed for multiplexed optical fiber sensor networks with built-in intelligence for detecting and discriminating between different metal ions in solution.     

A strategy to sequence repetitive DNA based on partial restriction enzyme cleavage

The strategy to sequence repetitive DNA described in this article is based on partial restriction enzyme cleavage. It is an alternative to using nested deletion with exonuclease III or similar enzymes in which progressively more remote regions of the target DNA are brought into range for sequencing by universal primers.     

<Emphasis Type="Italic">DegP</Emphasis> and Related Genes as Stress-Markers for <Emphasis Type="Italic">E.Coli</Emphasis>-Viability – Ultra-Sensitive RT-Real-Time PCR

Summary. The study was done to set up an on-line viability test system based on the stress-response of pathogenic E.coli. Fully automated and computerized pathogen detection was achieved via RNA expression monitoring using an high-speed real-time PCR-system with single molecule sensitivity. The device was developed in a collaboration of SAUR, France, the TU Delft (Kluyver Laboratorium – Analytical Biotechnology, Netherlands) and Attophotonics Bioscience, Austria.The automated viability assay is fully hands-off, using a continuous concentration process of the pathogens from litres sample down to tens of millilitres, cell cracking to DNA/RNA solution and further concentration down to a few hundred microlitres. RT-PCR and real-time-PCR are done with a robotic system. DegP found from E.coli to human was selectively induced within the device via heat shock at 50°C. Up to 1000 fold induction was achieved from environmental to heat-shock level. It was proven that degP quantification via RT-real-time PCR provides an excellent basis for multi-organism viability detection.Received September 16, 2002; accepted February 20, 2003 Published online September 15, 2003