单链构象多态性-毛细管电泳分析中样品纯化研究

聚合酶链反应(PCR)样品中的Cl^-、引物等小分子对单链构象多态性一毛细管电泳分析有重要影响。本文用毛细管电泳一间接紫外法测定了PCR样品中的Cl^-浓度,并对乙醇沉淀法和试剂盒纯化法用于PCR样品的纯化效果进行比较,在此基础上研究了Cl^-浓度、引物及其二聚体在SSCP-CE分析中的影响。结果表明,它们主要影响DNA进样量而对分离效率影响不大。     

邮递和保存质粒DNA的简易有效方法

邮递和保存质粒ＤＮＡ的简易有效方法程汉华，周荣家（武汉大学生命科学学院，武汉，４３００７２）中国法分类号Ｑ７８１随着现代分子生物学研究工作的不断深入和发展，国家或地区间的科技交流活动日趋平凡．常常需要在不同国家或地区的实验室之间邮递或携带质粒ＤＮＡ．...     

光纤通信中有色散影响的受激喇曼散射信道特性研究

提出了在波分复用光纤通信系统中，考虑光纤色散“走离”时受激喇曼散射(SRS)信道的新模型,给出了计算中距离步长的选取公式。根据该模型，数值计算了各信道在随机数字序列调制下和在受激喇曼散射(SRS)非线性效应作用下经过有色散“走离”的波分复用光纤系统的传输功率。得出光纤色散会降低SRS效应引起的输出功率波动的结论,画出了在SRS效应作用下输出功率标准差随色散系数变化的曲线，并对产生该现象的机理进行了定性分析。该模型适合于任意光纤色散、任意输入功率和任意信道数目。     

利用交叉增益调制实现光时分复用波分复用波长变换

采用半导体光放大器的交叉增益调制效应 ,通过全光方法 ,实现了 1.2Gbit/s光时分复用信号转换成2× 6 2 2Mbit/s波分复用信号的解复用。它同样具有波分复用信号到光时分复用信号波长变换的功能。     

Novel Real-Time Fluorescent PCR Chip Applied to Examine the Ankylosing Spondylitis

In this article, we introduce a novel real-time polymerase chain reaction (PCR) chip, which integrated the pretreatment of biological sample, the PCR reaction, and the real-time fluorescent detection. We have built a dam in the chamber of the chip and formed a crack underneath it to separate the white blood cell from the whole blood. After the lysis of white blood cells, which were separated from the whole blood, the real-time PCR reaction was produced by the integrated microheaters and resistance temperature detector (RTD), and the real-time fluorescent measurement was made in the identical chamber. Compared with conventional tubular PCR analysis, this chip-based PCR analysis can obtain more accurate results with a smaller amount of samples and reagents. We have designed the corresponding detection and control platform, which is composed of syringe pump module, temperature control module, and fluorescence sampling module. We also used the constructed chip for the examination of HLA-B27 related to ankylosing spondylitis, and the results show that the chip is available for the accurate and rapid analysis of HLA-B27 in whole blood.     

Quantification of Textile Dyes in Industrial Effluent Using UV-Vis Spectrophotometry Combined with Principal Components Regression

UV-Vis spectrophotometry and multivariate calibration model, Principal Components Regression (PCR), were used for individual and simultaneous quantification of ternary mixtures of reactive dyes (Yellow Procion HE4R, Navy Blue Procion HER, and Blue Procion HEGN) in a sample of industrial effluent and effluents obtained in the laboratory. A Centroid-Simplex-type experimental design was used. The percentages of the dyes Yellow Procion HE4R, Navy Blue Procion HER, and Blue Procion HEGN not fixed to the fiber in the industrial dyeing process and laboratory scale were 47.40%, 10.99%, 24.72% and 4.20%, 46.06%, 32.04%, respectively.     

Development of the 16 X‐STR loci typing system and genetic analysis in a Shanghai Han population from China

This study developed a new multiplex PCR system that simultaneously amplifies 16 X‐STR loci in the same PCR reaction, and the polymorphism and mutation rates of these 16 X‐STR loci were explored in a Shanghai Han population from China. These loci included DXS10134, DXS10159, DXS6789, DXS6795, DXS6800, DXS6803, DXS6807, DXS6810, DXS7132, DXS7424, DXS8378, DXS9902, GATA165B12, GATA172D05, GATA31E08, and HPRTB. Samples from 591 unrelated individuals (293 males and 298 females) and 400 two‐generation families were successfully analyzed using this multiplex system. Allele frequencies and mutation rates of the 16 loci were investigated, with the comparison of allele frequency distributions among different populations performed. Polymorphism information contents of these loci were all >0.6440 except the locus DXS6800 (0.4706). Nine cases of mutations were detected in the 16 loci from the investigation of 9232 meioses. Pairwise comparisons of allele frequency distributions showed significant differences for most loci among populations from different countries and ethnic groups but not among the Han population living in other areas of China. These results suggest that the 16 X‐STR loci system provides highly informative polymorphic data for paternity testing and forensic identification in the Han population in Shanghai, China, as a complementary tool.     

ResonSense: simple linear fluorescent probes for quantitative homogeneous rapid polymerase chain reaction

Here we report a strand-specific fluorescent homogeneous assay format for rapid polymerase chain reaction (PCR). A number of similar assays are commonly used for research applications and are an ideal solution for a closed tube quantitative PCR. These assays use fluorescent resonant energy transfer (FRET) between donor and acceptor fluorescent moieties as the reporting mechanism. However, for different reasons these assays do not report amplification when very rapid cycling times are used. This is because current assays, such as TaqMan^®, are limited, in terms of assay speed, by the 5′-3′ exonuclease activity of Taq DNA polymerase. Other assays based on hybridisation require either a complex de-conformational event to occur, or require more than one probe to report amplification. Reducing the complexity of the experiment reduces costs in terms of design, optimisation and manufacture. Here, we describe ResonSense^® chemistries that use a simple linear fluorescent-labelled probe and a DNA minor-groove binding dye as either donor or acceptor moieties in a homogeneous assay format on the LightCycler^®. This assay format will provide for rapid analysis of samples and so it is particularly well suited to point-of-use testing.