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31.
C. Gonalves Joaquim C.G. Esteves da Silva M.F. Alpendurada 《Analytica chimica acta》2006,560(1-2):164-171
An extensive monitoring programme of pesticides was carried out in soil samples from an intensive horticulture area in north of Portugal, putting into practice the needs for increased control of soil quality as far as organic pollution is concerned. The area under investigation was additionally defined as vulnerable to nitrates due to local soil and aquifer characteristics, which might be extended to pesticides contamination. Five sampling sites were selected and soils analysed at three depths in eight sampling campaigns, for the period of 2 years. A stepwise multivariate statistical approach was selected to uncover most relevant patterns inside a complex environmental data matrix. Cluster analysis was applied both to group pesticides and samples, giving a primary and unsupervised overlook of privileged relationships. Clusters of persistent pesticides and selected herbicides were identified, whereas sample classes were also formed and disposed geographically. Thirty eight percent of analysed soils samples fell into one class characterized by low contamination (class 1 in cluster analysis), which is entirely representative of the sampling site no. 1. Afterwards, linear discriminant analysis was applied to identify those pesticides, which had a higher impact in the definition of classes. Finally, factor analysis using a five component model was implemented in order to bring to light the constitution and data variance explained by each of the five main principal components, as well as, their relation to pest management practices. A factor was identified (PC1 – 22% variance) composed of chlorinated pesticides, which was representative of one of the investigated sites indicating its high contamination status. Qualitative main findings and class average concentration values were obtained through this multivariate statistical approach. 相似文献
32.
Yvonne C. Martin Elizabeth B. Danaher Catherine S. May David Weininger 《Journal of computer-aided molecular design》1988,2(1):15-29
Summary MENTHOR is a database system for the storage and retrieval of three-dimensional coordinate and charge information on molecules as well as of traditional biological and physical properties. Our molecular graphics system retrieves from MENTHOR structural information in individual molecules and receptor map/macromolecular binding site hypotheses. Substructural searches of MENTHOR are used to find starting coordinates for molecular modeling and traditional database searches of MENTHOR identify compounds for which modeling is needed. It also forms the data to be searched with ALLADDIN, our substructure/geometric search program. MENTHOR expedites molecular modeling by organizing previous work and facilitating transmission of information between individuals. Examples from modeling of D-2 receptor agonists are shown. 相似文献
33.
一种基于流动注射梯度技术识别异常峰及校正的方法 总被引:1,自引:0,他引:1
基于流动注射梯度信息提出了一种利用梯度比均值进行定量的校正方法。方法具有在线自动判别和修复异常峰并自行校正的功能,和通用的标准系列定量方法相比,两者测定精度相当。但本文提出的方法抗干扰能力明显优于后者,可适用于在线过程监测。 相似文献
34.
Haifang Liu Yuanqiang Sun Zhaohui Li Ran Yang Jie Yang Aaron Albert Aryee Xiaoge Zhang Jia Ge Lingbo Qu Yuehe Lin 《中国化学快报》2019,30(9):1647-1651
Intracellular pH plays a significant role in various biological processes, including cell proliferation, apoptosis, metabolism, enzyme activity and homeostasis. In this work, a novel design strategy for the preparation of pH responsive carbon dots (CDs-pH) for ratiometric intracellular imaging was reported. By using SciFinder database, fluorescent CDs-pH with the required pKa value of 6.84 were rationally designed, which is vital important for precise sensing of intracellular pH. As a result, the synthesized CDs-pH demonstrated robust ability to test pH fluctuations within the physiological range of 5.4-7.4. The CDs-pH was further utilized for fluorescent ratiometric imaging of pH in living HeLa cells, effectively avoided the influence of autofluorescence from native cellular species. Moreover, real-time monitoring of intracellular pH fluctuation under heat shock was successfully realized. This SciFinder-guided design strategy is simple and flexible, which has a great potential to be used for the development of other types of CDs for various applications. 相似文献
35.
A simple high-performance liquid chromatographic method was developed for the simultaneous determination of the therapeutic levels of acyclovir and ganciclovir in human plasma. After precipitation of plasma proteins with 6% perchloric acid, acyclovir and ganciclovir were simultaneously determined by reversed-phase chromatography with spectophotometric detection at 254 nm. The peak heights for acyclovir and ganciclovir were linearly related to their concentrations ranging from 0.063 to 2.080 micro g/mL. The recovery was 100.48-102.84% for acyclovir and 99.26-103.07% for ganciclovir. The intra- and inter-day relative standard deviation values were in the range 0.186-8.703% for acyclovir and 0.137-6.424% for ganciclovir. The detection limits for both compounds were 0.01 micro g/mL determined as the signal-to-noise ratio of 3. The present method is applicable to therapeutic monitoring during antiviral medication. 相似文献
36.
利用商品化ITO玻璃导电层的温阻效应, 无需任何微加工手段, 实现了自加热和传感的芯片温度自动程序控制, 最大程度地减小了传感滞后对温度控制稳定性的影响, 温度控制的稳定性达到了0.2 ℃, 升温速度最快可达20 ℃/s以上, 在冷却风扇辅助下降温速度最快达到了8 ℃/s. 芯片温控单元的引线从传统的两对(一对用于传感, 一对用于加热)减少为一对. 通过在该芯片上直接构建多个开放微池反应器的方法成功地实现了λDNA 157 bp片段的并行扩增. 将该芯片置于倒置荧光显微镜样品台上, 以蓝色(575 nm)发光二极管为光源, 以光电倍增管为检测手段检测了dsDNA和SYBR Green Ⅰ嵌合物的荧光强度随温度的实时变化曲线. 相似文献
37.
工业废水中微量铜(Ⅱ)和氰化物的流动注射光度分析 总被引:2,自引:0,他引:2
采用FIA技术,以光度比色法为基础,建立了工业废水中微量Cu^2+和CN^-的顺序自动分析方法和装置。其对二种离子的测定频率为60样/h,相对标准偏差<1.0%,工作曲线的r值≥0.999,各项分析技术指标均优于手工法操作。 相似文献
38.
Weil Joseph Brewer Mark Hendrickson Richard Sarikaya Ayda Ladisch Michael R. 《Applied biochemistry and biotechnology》1998,(1):99-111
Yellow poplar wood sawdust consists of 41% cellulose and 19% hemicellulose. The goal of pressure cooking this material in
water is to hydrate the more chemically resistive regions of cellulose in order to enhance enzymatic conversion to glucose.
Pretreatment can generate organic acids through acid-catalyzed degradation of monosaccharides formed because of acids released
from the biomass material or the inherent acidity of the water at temperatures above 160°C. The resulting acids will further
promote the acid-catalyzed degradation of monomers that cause both a reduction in the yield and the formation of fermentation
inhibitors such as hydroxymethyl furfural and furfural. A continuous pH-monitoring system was developed to help characterize
the trends in pH during pretreatment and to assist in the development of a base (2.0 M KOH) addition profile to help keep
the pH within a specified range in order to reduce any catalytic degradation and the formation of any monosac-charide degradation
products during pretreatment. The results of this work are discussed. 相似文献
39.
Linsinger TP Gawlik BM Trapmann S Lamberty A Emons H 《Analytical and bioanalytical chemistry》2004,378(5):1168-1174
Over the years, the nature of CRMs has changed considerably. Recently, more and more CRMs have been certified in their "natural" form, that is processed as little as possible, with analytes at their natural concentration level. This and the trend towards certified properties other than the concentrations of clearly defined molecules/elements have made guaranteeing stability of CRMs and estimating a shelf life an even more important issue for reference material producers than it has been before. One way to meet this challenge is to take more care in processing, storage and dispatch of CRMs. At IRMM, approximately 20 % of the RMs are stored at –20 °C or below and about 10% require cooled transportation. In addition, increased efforts for assessing stability are needed. Shelf lives are estimated using addition of an uncertainty component based on real-temperature stability studies rather than by accelerated stability studies. These pre-certification efforts are complemented by a stability-monitoring program, which at IRMM includes 80 % of the non-nuclear and non-isotopic materials. Although the costs for these efforts are high in absolute terms, they are only a minor and indispensable contribution to the total costs of CRM production. 相似文献
40.
Using column-switching liquid chromatography/tandem mass spectrometry (LC-MS/MS), we developed an improved analytical method of urinary estriol glucuronides. This new method is derived predominantly from maternal and fetal precursors in pregnancy. We used in the following procedure: first, we filtered urine samples with a membrane filter. Next, we directly injected the 50 microL aliquot of urine samples onto a pre-column. Then, after activating the column-switching valve, we backflushed the loaded samples onto the C(18) analytical column. Urine samples can be assayed within 20 min without any sample preparation steps. We monitored separated estriol glucuronides by negative electrospray ionization (ESI) and selected-reaction monitoring (SRM). The calibration range of estriol-3-glucuronide (E3-3G) and estriol-16-glucuronide (E3-16G) was 0.1-20 microg/mL and the linearity of the method was 0.9984 for E3-3G and 0.9987 for E3-16G. The limits of detection at a signal-to-noise (S/N) ratio of 3 were 10 ng/mL (E3-3G) and 5 ng/mL (E3-16G). The analytical recovery was over 85% and, in general, inter-day and intra-day variability for precision and accuracy were less than 10%. When applied to a pregnancy urine sample to biomedical monitoring of the function of the maternal/fetal unit, the proposed method allowed rapid and sensitive screening for the detection of E3-3G and E3-16G. 相似文献