Protonated dye-surfactant ion pair formation between neutral red and anionic surfactants in aqueous submicellar solutions

Spectral and surface tension behavior of aqueous neutral red in the presence of sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS) and sodium dodecyl sulfonate (SDSN) have been studied to understand the nature of the interactions in their submicellar concentration ranges. The variations in spectra and surface tension with variation in the concentrations of the surfactants suggest the formation of a 1:1 close-packed dye-surfactant ion pair, HNR⁺S⁻ between the acid form, HNR⁺ of the dye and the surfactant anion at very low concentrations of the surfactant below critical micelle concentration (cmc) of the pure surfactant. The dye-surfactant ion pair behaves like a nonionic surfactant having higher efficiency and lower cmc than that of the corresponding pure anionic surfactant. The ion pairs are adsorbed on the air/water interface at very low concentrations of the surfactant. As the concentration of the surfactant increases and the ion pairs form micelles of their own, the dye in the ion pair is protonated to form H₂NR²⁺S⁻. As the cmc of the pure surfactant is approached, the protonation equilibrium gradually reverses and pure surfactant ions gradually replace the ion pairs at the interface. Finally, a homogeneous monolayer of pure surfactant anions exists at the air/water interface and the dye remain solubilized in pure micelles above the cmc of the pure surfactant. The equilibrium constants, K_c for the close-packed protonated dye-surfactant ion pair (PDSIP) formation have been determined at varying pH. The submicellar interaction has been found to be stronger with SDS than SDBS. The plots of logarithm of K_c vs. pH have been found to be quite linear which consolidates the assumption of formation of the species, H₂NR²⁺S⁻. The interaction is driven by enthalpy as well as entropy.     

堆载下单桩负摩阻力工作性状非线性数值分析

利用三维非线性数值方法对一算例在堆载作用下桩侧负摩阻力进行了计算分析。计算结果揭示了摩擦型、端承型桩负摩阻力工作性状的异同; 分析了堆载速度、桩顶荷载对负摩阻力的影响: 堆载越快,负摩阻力越小; 在无桩顶荷载作用下,由负摩阻力引起的下拉荷载最大,可视为常规方法设计的上限值; 桩顶荷载与堆载施工顺序对负摩阻力的影响也很大,先施加桩顶荷载,后进行堆载所产生的负摩阻力最大,反之最小。     

General decay asymptotic stability of neutral stochastic differential delayed equations with Markov switching

The p-th moment and almost sure stability with general decay rate of the exact solutions of neutral stochastic differential delayed equations with Markov switching are investigated under given conditions. Two examples are provided to support the conclusions.     

Investigating Electrochemical Stability and Reliability of Gold Electrode‐electrolyte Systems to Develop Bioelectronic Nose Using Insect Olfactory Receptor

This article aims to demonstrate an electrochemically stable and reliable gold electrode‐electrolyte system to develop an insect odorant receptor (Drosophila melanogaster Or35a) based bioelectronic nose. Cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS) of bare gold electrodes, after modification with the self‐assembled monolayer (SAM) of 6‐mercaptohexanoic acid (MHA) and after immobilization with Or35a integrated into the lipid bilayers of liposomes were conducted in the presence of four different redox probes. Potassium ferri/ferrocyanide [Fe(CN)₆]^3?/[Fe (CN)₆]^4? and hydroquinone (H₂Q) redox probes revealed variable and irreversible signals at the time scale of our measurements, with atomic force microscopy (AFM) images and x‐ray photoelectron spectroscopy (XPS) results suggesting gold surface etching due to the presence of CN^? ions in case of [Fe(CN)₆]^3?/[Fe (CN)₆]^4?. Although the hexaammineruthenium complex showed stable electrochemical behaviour at all stages of biosensor development, changes in CV and EIS readings after each surface modifications were insignificant. PBS buffer as a non‐Faradaic medium, was found to provide reliable systems for electrochemical probing of modified gold electrodes with Or35a/liposomes in aqueous media. Using this system, we have shown that this novel biosensor can detect its known odorant E2‐hexenal selectively compared to methyl salicylate down to femtomolar concentration.     

Absorption Spectrum of Neutral Krypton in the Near Infrared Region

High-resolution absorption spectra of atomic krypton in the range of 11870-12700 cm^?1 were recorded by employing concentration modulation absorption spectroscopy technique with a tunable single-mode cw Ti:Sapphire laser. The krypton atoms were excited to the absorbing energy states by discharge-burning in a mixture of helium and krypton. A total of 120 lines of neutral krypton were observed, among them 33 lines had already been classified in previous studies, 45 lines were newly classi ed with the known energy levels, and 42 lines cannot be classi ed. These unclassified lines indicate that up to now unknown energy levels of Kr must exist. Further, an analysis of the unclassi ed lines to get possible new energy levels with a classi cation program is reported.     

Probing Deviation of Adhered Membrane Dynamics between Reconstituted Liposome and Cellular System

The dynamics of cell‐cell adhesion are complicated due to complexities in cellular interactions and intra‐membrane interactions. In the present work, we have reconstituted a liposome‐based model system to mimic the cell‐cell adhesion process. Our model liposome system consists of one fluorescein‐tagged and one TRITC (tetramethyl‐rhodamine isothiocyanate)‐tagged liposome, adhered through biotin‐neutravidin interaction. We monitored the adhesion process in liposomes using Förster Resonance Energy Transfer (FRET) between fluorescein (donor) and TRITC (acceptor). Occurrence of FRET is confirmed by the decrease in donor lifetime as well as distinct rise time of the acceptor fluorescence. Interestingly, the acceptor's emission exhibits fluctuations in the range of ≈3±1 s. This may be attributed to structural oscillations associated in two adhered liposomes arising from the flexible nature of biotin‐neutravidin interaction. We have compared the dynamics in a cell‐mimicking liposome system with that in an in vitro live cell system. In the adhered live cell system, we used CPM (7‐diethylamino‐3‐(4‐maleimido‐phenyl)‐4‐methylcoumarin, donor) and nile red (acceptor), which are known to stain the membrane of CHO (Chinese Hamster Ovary) cells. The dynamics of the adhered membranes of two live CHO cells were observed through FRET between CPM and nile red. The acceptor fluorescence intensity exhibits an oscillation in the time‐scale of ≈1±0.75 s, which is faster compared to the reconstituted liposome system, indicating the contributions and involvement of multiple dynamic protein complexes around the cell membrane. This study offers simple reconstituted model systems to understand the complex membrane dynamics using a FRET‐based physical chemistry approach.     

关于《偶数阶中立型微分方程的线性化振动的一个注记》一文的注记

本文通过反例说明《偶数阶中立型微分方程的线性化振动的一个注记》一文中主要定理的错误（参见文[2]）.     

二阶线性中立时滞方程非振动解的存在性

考虑具有正负系数的中立时滞微分方程这里P∈R和τ∈(0,∞),σ1,σ2∈[0,∞)且Q1,Q2∈C([t0,∞),R+).对于上面方程非振动解的存在性,得到一个用,∫sQids <∞,i=1,2,来表达的充分条件。这个结果去掉了M.R.S.Kulenovic和S.Hadziomerspahic文中一个相当强的假设,改进了其中的相关定理.