荧光光谱法研究注射用盐酸头孢替安与牛血清白蛋白的相互作用

荧光光谱法研究了注射用盐酸头孢替安（Cefotiam Hydrochloride for Injection,CH）与牛血清白蛋白（BSA）的相互作用。盐酸头孢替安对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭,在291K时,求出了猝灭常数K_KV=2.49×10⁴L/mol、结合常数K_A=3.8×10³L/mol及结合位点数n=0.81。在同步荧光光谱中BSA色氨酸和酪氨酸发射峰红移,色氨酸和酪氨酸的微环境发生变化。     

近红外漫反射光谱法对益母草颗粒的定量和定性分析

利用近红外光谱结合偏最小二乘法建立了快速测定益母草颗粒的盐酸水苏碱的定量分析模型,模型的决定系数为0.9622,内部交叉验证均方差为0.0576;然后用10个预测集样本对模型的性能进行验证,决定系数为0.9495,预测集标准偏差为0.0547,相对分析误差为4.45,表明该方法能应用于生产实践。最后选取样品的近红外光谱的6502.8—3999.6cm-1波段建立9个厂家的益母草颗粒聚类分析模型,结果不同厂家的样品能很好地进行分类,然后使用预测集样品对该模型进行检验,结果表明该方法准确快速。     

Determination of metformin based on amplification of its voltammetric response by a combination of molecular wire and carbon nanotubes

Molecular wires containing copper(II) (CuMW), in the form of the coordination polymer (Cu(II)₄(bpp)₄(maa)₈(H₂O)₂).2H₂O (bpp=1,3-bis(4-pyridyl)propane, maa=2-methylacrylic acid), and multiwalled carbon nanotubes (CNT) have been combined to prepare a paste electrode (CuMW/CNT/PE). The voltammetric response of the CuMW/CNT/PE to metformin (MET) was significantly greater than that of electrodes prepared from other materials, because of both the surface effect of CuMW and CNT and coordination of MET with the Cu(II) ion in the CuMW. A novel voltammetric method for determination of MET is proposed. In pH 7.2 Britton–Robinson buffer, using single sweep voltammetry, the second-order derivative peak current for oxidation of MET at 0.97 V (relative to SCE) increased linearly with MET concentration in the range 9.0 × 10⁻⁷–5.0 × 10⁻⁵ mol L⁻¹ and the detection limit was 6.5 × 10⁻⁷ mol L⁻¹. Figure When a combination of molecular wires containing copper(II) (CuMW) and multiwalled carbon nanotubes (CNT) was used to prepare a paste electrode (CuMW/CNT/PE) the voltammetric response to metformin (curve c) was significantly higher than that at a carbon/PE (curve a) or a CNT/PE (curve b), because of the amplification effect of CNT and CuMW. A novel voltammetric method is proposed for determination of MET     

Quantum Chemical Study on the adsorption of metformin drug on the surface of pristine,Si- and Al-doped (5, 5) SWCNTs

Adsorption properties of metformin (MF) drug onto pristine, Si- and Al-doped (5, 5) armchair single-wall carbon nanotubes (SWCNTs) were studied using density functional theory (DFT) calculations at the B3LYP and ωB97XD methods with the standard 6–311 G** basis set. The most stable geometries of the MF drug molecule onto pristine, Si- and Al-doped (5, 5) CNTs were selected and evaluated in the gaseous and aqueous environments. We calculated the natural bond orbitals (NBO), Frontier molecular orbital (FMO), density of states (DOS) and molecular electrostatic potential (MEP) of systems upon adsorption of MF drug. It was found that the reaction of MF drug with pure SWCNT is physisorption in nature, while high chemisorption can be achieved by using Al- and Si-doped SWCNTs. Despite Al-doped SWCNT provides stronger adsorption, however the change in the energy gap of Si-doped SWCNT is more pronounced. It is predicted that MF drug incorporating Si-doped SWCNT can be extended as drug delivery system.     

盐酸美金刚胺含量分析方法的研究

在一定 p H值下 ,铵离子和二价铜离子反应 ,生成络合物在紫外有最大吸收 ,吸收波长为 36 5 nm,建立一种简便可靠的盐酸美金刚胺分析方法。盐酸美金刚胺在 1 0 0— 5 0 0 μg· m L-1范围内呈良好的线性关系 ,回归方程为 A=0 .0 0 2 6 C(r=0 .9987)。平均回收率为 1 0 0 .7%(n=6 ) ,相对标准偏差为 1 .37%。本方法简便、可靠、准确。     

示波极谱滴定法测定盐酸氯喘等药物

盐酸氯喘,盐酸阿米替林和维生系B_6都是常用药物,中国药典和上海市药品标准用非水滴定法测定它们的含量,比较麻烦。梁云爱等用示波滴定法测定维生素B_6的含量,采用NaoH中和滴定测定盐酸吡多醇中的盐酸部分。我们在一定pH值的醋酸—     

分光光度法测定黄瓜籽粉中盐酸小檗碱的含量

采用超声波法在2种条件下即(1)先超声处理,后放置过夜;(2)先放置过夜,后超声处理,对黄瓜籽粉中盐酸小檗碱进行提取,利用分光光度法测定盐酸小檗碱的含量,检测波长为426nm。回归方程y=0.00393+1.28929x(mg/10mL),r=0.9999;盐酸小檗碱浓度在0.099—0.796mg/10mL范围内与吸光度呈线性关系。2种方法的平均含量分别为2.4043、1.4797mg/g。平均回收率分别为94.96%、107.32%。实验结果表明,方法(1)较方法(2)提取率高。分光光度法用于黄瓜籽粉中盐酸小檗碱的含量测定,简便,可靠,快捷。