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71.
《Soft Materials》2013,11(2-3):85-108
Abstract

Liposomes containing rigid and bulky lipid molecules such as sterols and pyrene‐labeled lipids can exhibit biphasic changes in membrane properties at several specific mole fractions predicted by the theory of lipid regular distributions (e.g., superlattices) in the plane of the membrane. This phenomenon has been observed in two‐component as well as multicomponent liquid‐crystalline liposomal membranes. The extent of sterol regular distribution plays a role in drug partitioning into membranes, the activities of surface‐acting enzymes such as cholesterol oxidase and phospholipase A2, and in free‐radical‐induced sterol oxidation. This article summarizes the original fluorescence studies of lipid superlattices, reviews the recent findings in this area, and discusses the current controversial issues related to lipid regular distributions.  相似文献   
72.
The main focus of this paper is to examine the consequence of laser pulses of narrow width impinging on phosphatidylcholine liposomes containing sulforhodamine dye molecules. The release of dye molecules following short-pulsed laser excitation and localized heating was measured and its dependence on laser excitation parameters studied. A characterization of the optimal conditions necessary for release of liposome contents can be applied to the targeted delivery of therapeutic drugs.  相似文献   
73.
The fluorescence quenching of fluorescein (FL) by merociyanine 540 (MC540) was examined in L-egg lecithin phosphatidycholine (PC) liposomes using spectroscopic methods. The type of quenching mechanism (dynamic or static) was evaluated using the Stern-Volmer plots. Findings were also supported by the temperature studies and florescence decay measurements. The Stern-Volmer equation was utilized to calculate bimolecular quenching constants (Kq). Furthermore, the bimolecular quenching constant of the quencher in the liposomes (KSV), partition coefficient (Kp), binding constant (K), and corresponding thermodynamic parameters ΔH, ΔS, and ΔG were calculated. The quenching property was also used in determining quantitatively (Kp) the partition coefficient of Merociyanini 540 in PC liposome.The obtained data indicated that static quenching occurred in the system and the KSV values decreased with increasing lipid concentration. In addition, thermodynamic analysis suggested that van der Waals interactions and hydrogen bonding were the main acting forces between fluorescein and merociyanine 540 molecules in the medium.  相似文献   
74.
75.
Curcumin shows huge potential as an anticancer and anti-inflammatory agent. However, to achieve a satisfactory bioavailability and stability of this compound, its liposomal form is preferable. Our detailed studies on the curcumin interaction with lipid membranes are aimed to obtain better understanding of the mechanism and eventually to improve the efficiency of curcumin delivery to cells. Egg yolk phosphatidylcholine (EYPC) one-component monolayers and bilayers, as well as mixed systems containing additionally dihexadecyl phosphate (DHP) and cholesterol, were studied. Curcumin binding constant to EYPC liposomes was determined based on two different methods: UV/Vis absorption and fluorescence measurements to be 4.26 × 104 M−1 and 3.79 × 104 M−1, respectively. The fluorescence quenching experiment revealed that curcumin locates in the hydrophobic region of EYPC liposomal bilayer. It was shown that curcumin impacts the size and stability of the liposomal carriers significantly. Loaded into the EYPC/DPH/cholesterol liposomal bilayer curcumin stabilizes the system proportionally to its content, while the EYPC/DPH system is destabilized upon drug loading. The three-component lipid composition of the liposome seems to be the most promising system for curcumin delivery. An interaction of free and liposomal curcumin with EYPC and mixed monolayers was also studied using Langmuir balance measurements. Monolayer systems were treated as a simple model of cell membrane. Condensing effect of curcumin on EYPC and EYPC/DHP monolayers and loosening influence on EYPC/DHP/chol ones were observed. It was also demonstrated that curcumin-loaded EYPC liposomes are more stable upon interaction with the model lipid membrane than the unloaded ones.  相似文献   
76.

Diamagnetic dipalmitoylphosphatidylcholine (DPPC) liposomes dispersed in glucose solution as well as their paramagnetic analogs encapsulating a paramagnetic contrast agent used in magnetic resonance imaging (Gd-HPDO3A, ProHance ® ) were prepared and characterized. The vesicle diameter was assessed by photon correlation spectroscopy (PCS). 31 P NMR spectroscopy was used to measure the phospholipid content and to confirm the highly unilamellar character of the liposome membrane. For both types of liposome preparation, the internal water volume was evaluated below the phase transition temperature ( T m ) by natural abundance 17 O NMR spectroscopy in the presence of a shift reagent confined to the external compartment. For the paramagnetic vesicles, the internal water content was independently assessed by analysis of the biexponential decay of the proton transverse magnetization below T m . Knowing the unilamellarity of the vesicles ( 31 P NMR measurements), the number concentration of liposomes was assessed from the liposomal internal volume calculated from PCS data and the total internal water content obtained by 17 O NMR spectroscopy or 1 H relaxometry. The results obtained are in good agreement and validate these techniques as non invasive methods for the assessment of the number concentration of liposome in suspension.  相似文献   
77.
Liposomes have now evolved into a commercially-important drug delivery vehicle by overcoming a host of problems that were initially encountered with first generation liposomes. In spite of these impressive advances, the great potential of liposomes as drug delivery vehicles will not be fully realized until more effective targeting and membrane fusion mechanisms have been incorporated into their formulations. Our laboratory has developed several plasmenyl-type lipids for use in acid- or photooxidatively-triggerable liposomes. This review summarizes our progress toward the design, synthesis, and triggered release of encapsulated agents upon acid-catalyzed hydrolysis or photosensitized oxidation of plasmenyl-type lipid systems. Application of these materials in cascade triggering and intracellular drug delivery schemes is also described.  相似文献   
78.
The electric field correlation function of light scattered from a polydispersed population of spherical particles having log-normal distribution with varying polydispersity is simulated. The correlation function with different polydispersity is compared with the method of cumulants over a wide range of correlation time. The large positive deviation of the method of cumulants at long correlation time is identified. This necessitates the truncation of the data at long correlation time or use of an appropriate weighting function to eliminate errors in the analysis. A modified cumulant analysis is used to overcome the limitation of truncating the correlation function. QELS data from polydisperse samples of micelles, liposomes and polyaniline nanoparticles are compared using the two methods. This method can be extended to the analysis of other multi-exponential decays such as stress relaxation, positron annihilation and NMR relaxation.  相似文献   
79.
Gold nanoparticles were loaded in the bilayer of dipalmitoylphosphatidylcholine (DPPC) liposomes, named as gold-loaded liposomes. Above the gel to liquid-crystalline phase transition temperature, membrane fluidities of DPPC liposomes were changed by loading the gold nanoparticles. Compared with liposomes without loading the gold nanoparticles, gold-loaded liposomes showed the lower fluorescence anisotropy values. That is, the membrane fluidities of DPPC bilayer were increased by loading the gold nanoparticles. The membrane fluidities were increased as the amount of gold nanoparticles increased. The existence of gold nanoparticles in the DPPC bilayer was observed by transmission electron microscopy. Through the energy dispersive X-ray spectrometer, the particles in DPPC bilayer were confirmed to be gold nanoparticles.  相似文献   
80.
This work reports the development of an automatic methodology based on the use of 1-anilinonaphthalene-8-sulfonate (ANS) as an interfacial fluorescent probe for detecting the hydrophobic environment shift around the probe, caused by the hydrolytic action of PLA2 on the liposomes. The implementation of this reaction in a sequential injection analysis (SIA) system along with the use of the mixing chambers permitted the evaluation of PLA2 activity and assessment of the inhibitory effect of the non-steroidal anti-inflammatory drugs (NSAIDs) on PLA2 activity.Several studies were performed with the aim of establishing the appropriate flow system configuration: the liposome substrate; PLA2 and ANS optimum concentrations and incubation times before and after the enzyme addition. Based on these studies, the optimum reaction conditions were selected. It was shown that PLA2 is effectively inhibited by the NSAIDs tested (meloxicam, tolmetin and ibuprofen) and by the α-lipoic acid, used as a positive control.Results obtained from the flow system are in agreement with those provided by the comparison batch procedures. The proposed methodology is in fact more efficient and rapid than the comparison batch experiments, enabling the exact timing of fluidic manipulations and precise control of the reaction conditions.  相似文献   
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