排序方式: 共有50条查询结果,搜索用时 12 毫秒
41.
Danyang Wang Shubo Du Dr. Amaury Cazenave-Gassiot Dr. Jingyan Ge Prof. Dr. Jun-Seok Lee Prof. Dr. Markus R. Wenk Prof. Dr. Shao Q. Yao 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(21):5923-5927
The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein–phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated. 相似文献
42.
Dr. Clève D. Mboyi Dr. Delphine Vivier Ahmad Daher Prof. Dr. Paul Fleurat-Lessard Dr. Hélène Cattey Dr. Charles H. Devillers Dr. Claire Bernhard Prof. Dr. Franck Denat Dr. Julien Roger Prof. Dr. Jean-Cyrille Hierso 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(3):1165-1170
Click chemistry at a tetrazine core is useful for bioorthogonal labeling and crosslinking. Introduced here are two new classes of doubly clickable s-aryl tetrazines synthesized by Cu-catalyzed cross-coupling. Homocoupling of o-brominated s-aryl tetrazines leads to bis(tetrazine)s structurally characterized by tetrazine cores arranged face-to-face. [N]8 π-stacking interactions are essential to the conformation. Upon inverse electron demand Diels–Alder (iEDDA) cycloaddition, the bis(tetrazine)s produce a unique staple structure. The o-azidation of s-aryl tetrazines introduces a second proximal intermolecular clickable function that leads to double click chemistry opportunities. The stepwise introduction of fluorophores and then iEDDA cycloaddition, including bioconjugation to antibodies, was achieved on this class of tetrazines. This method extends to (thio)etherification, phosphination, trifluoromethylation and the introduction of various bioactive nitrogen-based heterocycles. 相似文献
43.
《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2018,130(7):1957-1961
Thionyl tetrafluoride (SOF4) is a valuable connective gas for sulfur fluoride exchange (SuFEx) click chemistry that enables multidimensional linkages to be created via sulfur–oxygen and sulfur–nitrogen bonds. Herein, we expand the available SuFEx chemistry of SOF4 to include organolithium nucleophiles, and demonstrate, for the first time, the controlled projection of sulfur–carbon links at the sulfur center of SOF4‐derived iminosulfur oxydifluorides (R1−N=SOF2). This method provides rapid and modular access to sulfonimidoyl fluorides (R1−N=SOFR2), another array of versatile SuFEx connectors with readily tunable reactivity of the S−F handle. Divergent connections derived from these valuable sulfonimidoyl fluoride units are also demonstrated, including the synthesis of sulfoximines, sulfonimidamides, and sulfonimidates. 相似文献
44.
《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2018,130(33):10654-10659
The bioorthogonal cleavage of allylic carbamates from trans‐cyclooctene (TCO) upon reaction with tetrazine is widely used to release amines. We disclose herein that this reaction can also cleave TCO esters, carbonates, and surprisingly, ethers. Mechanistic studies demonstrated that the elimination is mainly governed by the formation of the rapidly eliminating 1,4‐dihydropyridazine tautomer, and less by the nature of the leaving group. In contrast to the widely used p‐aminobenzyloxy linker, which affords cleavage of aromatic but not of aliphatic ethers, the aromatic, benzylic, and aliphatic TCO ethers were cleaved as efficiently as the carbamate, carbonate, and esters. Bioorthogonal ether release was demonstrated by the rapid uncaging of TCO‐masked tyrosine in serum, followed by oxidation by tyrosinase. Finally, tyrosine uncaging was used to chemically control cell growth in tyrosine‐free medium. 相似文献
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46.
《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(5):1293-1297
Three‐dimensional (3D) ordered arrays of human immunoglobulin G (IgG) were fabricated using well‐defined full‐length antibody–polymer conjugates (APCs). The conjugates were prepared through a two‐step sequential click approach with a combination of oxime ligation and strain promoted alkyne–azide cycloaddition. They were able to self‐assemble into lamellar nanostructures with alternating IgG and poly(N ‐isopropylacrylamide) (PNIPAM) nanodomains. As a proof‐of‐concept, these materials were fabricated into thin films and their specific binding ability was tested. The nanostructure not only improves the packing density and the proper orientation of the IgG, but also provides nanochannels to facilitate substrate transport. 相似文献
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48.
Manh Linh Nguyen Jaeduk Byun Suwoong Kim June Won Hyun Kahyun Hur Tae Joo Shin Byoung‐Ki Cho 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(9):2775-2779
The 1,2,3‐triazole molecule, which is a product of click chemistry, possesses a high dipole moment and can be a useful polar motif for ferroelectric columnar liquid crystal (LC) materials—though it has not been used to date. Herein, we report the helical assembly and ferroelectric switching properties of a columnar liquid crystal comprising a naphthalene core and 1,2,3‐triazolyl linkages. The molecule assembles into a double‐stranded helical columnar LC structure (Colhel). The X‐ray simulations of cisoid and transoid columnar models suggest that the helical assembly comprises cisoid conformers with a non‐zero dipole moment. The helical columns in the Colhel phase are aligned homeotropically under an electric field. The ferroelectric switching of the axial polarization can be observed in the temperature range of 105–115 °C in the Colhel phase, wherein the triazolyl hydrogen bonding along the column axis is weakened. The ferroelectric switching event is attributed to the rotation of the polar triazolyl units in response to the electric field. 相似文献
49.
Faming Wang Yan Zhang Zhengwei Liu Zhi Du Lu Zhang Jinsong Ren Xiaogang Qu 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(21):7061-7066
As a typical bioorthogonal reaction, the copper‐catalyzed azide–alkyne cycloaddition (CuAAC) has been used for drug design and synthesis. However, for localized drug synthesis, it is important to be able to determine where the CuAAC reaction occurs in living cells. In this study, we constructed a heterogeneous copper catalyst on a metal–organic framework that could preferentially accumulate in the mitochondria of living cells. Our system enabled the localized synthesis of drugs through a site‐specific CuAAC reaction in mitochondria with good biocompatibility. Importantly, the subcellular catalytic process for localized drug synthesis avoided the problems of the delivery and distribution of toxic molecules. In vivo tumor therapy experiments indicated that the localized synthesis of resveratrol‐derived drugs led to greater antitumor efficacy and minimized side effects usually associated with drug delivery and distribution. 相似文献
50.
Jorge Royes Oana Ilioaia Quentin Lubart Federica Angius Galina V. Dubacheva Marta Bally Bruno Miroux Christophe Tribet 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(22):7473-7477
Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high‐yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria‐based proteoliposomes are an attractive eco‐friendly alternative that can outperform currently used liposomes. 相似文献