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111.
The on-line incorporation of cloud point extraction (CPE) to flow injection analysis (FIA) is modified to extract and preconcentrate metal species rapidly, avoiding the formation of hydrophobic complexes, using a mixed micellar medium. Coupling the procedure with chemiluminescence (CL) detection based on the catalytic activity of metal species on the luminol-hydrogen peroxide reaction and enhancing the signal with the presence of a micellar carrier containing bromide ions produces a powerful tool for the preconcentration and determination of metal species at ng l−1 levels. As an analytical demonstration ultratrace concentrations of chromium were conveniently detected and quantified in samples with a complex matrix such as seawater and wastewater. The figures of merit for the determination of chromium were: 0.9-1.6% R.S.D. (n=5) with detection and quantification limits 0.5 and 2.0 ng l−1, respectively. The calibration graph was rectilinear from 2 to 200 ng l−1 (r=0.9996, n=6). Several other metal ions were determined in ideal situations, with analogous results. 相似文献
112.
以作者开发的从蛋白质结合部位推导出其界面所具有的疏水性质和氢键性质的计算程序PP_SITE为基础,利用蛋白质结构数据库(PDB),对蛋白质-蛋白质相互作用界面进行了统计分析.从PDB中挑出非冗余的链间相互作用对,计算出这个数据集中所有链间界面的疏水和氢键相互作用特征.对得到的界面特征进行统计分析,寻找能够明显聚类的界面特征.结果表明,界面大小、氢键和疏水相互作用在界面所占比例以及疏水相互作用的集中程度可以作为分类的依据. 相似文献
113.
Electrically conducting poly(3,4‐ethylenedioxythiophene) (PEDOT) film doped with silicomolybdate (SiMo12O404? or SiMo12) was synthesized by electrochemical polymerization. The synthesized film is capable of fast charge propagation during redox reactions in strong acid medium 0.2 M H2SO4 solution. The modified electrode was used towards reduction of bromate and successfully employed as an amperometric sensor for bromate and also above modified electrode was investigated for ascorbic acid oxidation. 相似文献
114.
Single cell analytics for proteomic analysis is considered a key method in the framework of systems nanobiology which allows a novel proteomics without being subjected to ensemble-averaging, cell-cycle, or cell-population effects. We are currently developing a single cell analytical method for protein fingerprinting combining a structured microfluidic device with latest optical laser technology for single cell manipulation (trapping and steering), free-solution electrophoretical protein separation, and (label-free) protein detection. In this paper we report on first results of this novel analytical device focusing on three main issues. First, single biological cells were trapped, injected, steered, and deposited by means of optical tweezers in a poly(dimethylsiloxane) microfluidic device and consecutively lysed with SDS at a predefined position. Second, separation and detection of fluorescent dyes, amino acids, and proteins were achieved with LIF detection in the visible (VIS) (488 nm) as well as in the deep UV (266 nm) spectral range for label-free, native protein detection. Minute concentrations of 100 fM injected fluorescein could be detected in the VIS and a first protein separation and label-free detection could be achieved in the UV spectral range. Third, first analytical experiments with single Sf9 insect cells (Spodoptera frugiperda) in a tailored microfluidic device exhibiting distinct electropherograms of a green fluorescent protein-construct proved the validity of the concept. Thus, the presented microfluidic concept allows novel and fascinating single cell experiments for systems nanobiology in the future. 相似文献
115.
A new high-performance liquid chromatographic (HPLC) method for measuring low molecular weight (LMW) thiol-containing compounds, including cysteine (CysH), glutathione (GSH), N-acetylcysteine (Nac), penicillamine (PA), and 2-mercaptoethanol (2-ME), has been developed by using 5-methyl-(2-(m-iodoacetylaminophenyl)benzoxazole (MIPBO) as fluorescence-labeling reagent. The derivatization and separation conditions have been investigated in detail. Detection limits ranging from 3.5 to 15.0 fmol were achieved for the thiols investigated in a 16 min separation with detection wavelengths 310 and 375 nm for the excitation and emission, respectively. The utility of the proposed method has been validated by measuring CysH in human urine samples. 相似文献
116.
In the context of a European project, a new approach of sampling of volatile polycyclic aromatic hydrocarbons (PAHs) from air was developed. In fact, the aim of this project was to test the efficiency of an air cleansing prototype reactor, which was operating by non-thermal plasmolysis. With an eye to model the atmosphere ejected by the prototype, we needed to vaporise the volatile PAHs in an air stream at concentrations as low as those recommended by European Directives (96/62/CE) for PAHs in ambient air (i.e. 1 ng m−3). Our strategy was based on the analysis of PAHs trapped in an aqueous medium, in order to avoid important losses of volatile compounds observed during the delicate desorption-concentration step when classical solid supports are used. Then a study was carried out to determine: the design of the collecting part, the flow-rate of the air sampling, the nature and concentration of chemical additives used to enhance PAH solubility in water. The very highly diluted aqueous media obtained after the bubbling step were concentrated by solid-phase extraction (SPE) on hydrophobic cartridges and analysed on-line by reversed-phase HPLC with UV and fluorimetric detections. Lastly, the sampling technique was directly applied to the outlet of the air cleansing prototype and the analysis after 3-6 h of non-thermal plasmolysis showed that the target volatile PAHs were not present in an air stream initially polluted by volatile organic compounds. 相似文献
117.
A simple high-performance liquid chromatographic method was developed for the simultaneous determination of the therapeutic levels of acyclovir and ganciclovir in human plasma. After precipitation of plasma proteins with 6% perchloric acid, acyclovir and ganciclovir were simultaneously determined by reversed-phase chromatography with spectophotometric detection at 254 nm. The peak heights for acyclovir and ganciclovir were linearly related to their concentrations ranging from 0.063 to 2.080 micro g/mL. The recovery was 100.48-102.84% for acyclovir and 99.26-103.07% for ganciclovir. The intra- and inter-day relative standard deviation values were in the range 0.186-8.703% for acyclovir and 0.137-6.424% for ganciclovir. The detection limits for both compounds were 0.01 micro g/mL determined as the signal-to-noise ratio of 3. The present method is applicable to therapeutic monitoring during antiviral medication. 相似文献
118.
119.
《Electroanalysis》2005,17(8):706-712
Capillary electrophoresis with amperometric detection was applied to determine some β2‐agonists, such as clenbuterol, cimaterol and salbutamol in this paper. The working electrode used was a 0.3 mm diameter carbon disk electrode. In this work, the pH 6.0–6.4 borax‐potassium dihydrogen phosphate was used as running buffer (150 mmol/L), 10 kV as the separation voltage and 1.05 V (vs. Ag/AgCl, 3 mol/L KCl) as the detection potential. Under the optimum conditions, the analytes were baseline separated within 16 min. Linear range for cimaterol, clenbuterol and salbutamol was 1.0–2000, 2.0–2000 and 1.0–2000 ng/mL, respectively. The detection limits (define as 3σ/k) were 0.5, 1.0 and 0.4 ng/mL for cimaterol, clenbuterol and salbutamol, respectively. The developed method has been applied to determine these three analytes in feed and urine by standard addition. The mean recoveries for these three analytes ranged from 89.0% to 102.0%. 相似文献
120.
Both absolute molecular weight and molecular sizes (radius of gyration and hydrodynamic radius) of a vinyl-type polynorbornene eluting from size-exclusion chromatography columns were determined by combined with a static and dynamic laser light scattering detector. The hydrodynamic radius of polymer fraction eluting from size-exclusion chromatography columns was obtained from dynamic laser light scattering measurements at only a single angle of 90° by introducing a correction factor. According to the scaling relationship between molecular sizes and molecular weight and the ratio between radius of gyration and hydrodynamic radius, the vinyl-type polynorbornene took a random coil conformation in 1,2,4-trichlorobenzene at 150 °C. 相似文献