A data base for polymer chromatography: Temperature dependence of interaction parameters in liquid adsorption chromatography

The temperature dependence of retention behaviour of polyethylene glycol (PEG) and its mono‐ and dimethyl ethers was studied on various RP columns in different mobile phases. The accessible volumes and the interaction parameters were determined from slope and intercept in a plot of the elution volumes of the oligomers of a polymer homologous series as a function of the difference of the elution volumes of consecutive oligomers. A quite different dependence of the interaction parameters was observed in the different mobile phases. While in methanol–water the interaction parameter decreases with increasing temperature, the opposite effect is observed in acetonitrile (ACN)–water. In acetone–water, the temperature dependence is almost negligible.     

荧光猝灭法研究氟比洛芬与DNA相互作用

采用荧光光谱法和紫外光谱法, 在生理条件下(pH=7.40)对氟比洛芬(FP)与脱氧核糖核酸(DNA)的作用方式进行了研究.证实了氟比洛芬通过沟槽结合方式与DNA发生相互作用.实验表明, DNA与氟比洛芬的相互作用为静态猝灭过程,测得结合常数为5.53×10~5 L·mol~(-1),结合位点数为1.12.     

对硝基苯胺与牛血清白蛋白的相互作用研究

用荧光光谱和质谱研究了对硝基苯胺(PNA)与牛血清白蛋白(BSA)的相互作用.结果表明,对硝基苯胺能与BSA相结合,结合后对硝基苯胺的ESI-MS选择正离子峰明显减弱,并有规律地猝灭BSA的内源荧光,其机理属静态猝灭过程.实验获得了不同温度下,对硝基苯胺与BSA作用的结合常数和热力学参数,根据所得结果可推断对硝基苯胺与BSA的主要作用力为疏水作用力.由Frster非辐射能量转移理论计算得出了对硝基苯胺与BSA结合位置的距离.采用同步荧光研究发现,对硝基苯胺能进入BSA的疏水区,从而对BSA的构象产生一定的影响,这与对硝基苯胺的生物毒性有关.     

热重-差示扫描量热法研究金属铝盐与载体的相互作用

通过浸渍法将相同负载量的不同类型的金属铝盐负载在介孔分子筛HMS内外表面,用热重-差示扫描量热(TG-DSC)法研究了金属铝盐热性质的变化。将负载型金属铝盐干燥、焙烧,得到负载型催化剂Al2O3/HMS样品,用X射线衍射(XRD)、N2吸附-脱附等手段对样品进行了介孔结构表征,并初步考察了催化剂上烷基化反应的活性。实验结果表明:负载金属盐的起始分解温度均高于金属盐,证明了金属盐与载体之间有相互作用。Al2O3/HMS催化剂在对苯二酚烷基化反应中表现出不同的催化活性。这种催化活性的高低与催化剂比表面积、孔容的大小没有相应的顺变关系,与负载金属盐与载体的相互作用有关,相互作用越弱,催化剂在烷基化反应中表现出的催化活性越高。     

Enrichment and low‐level determination of glyphosate,aminomethylphosphonic acid and glufosinate in drinking water after cleanup by cation exchange resin

For the determination of glyphosate, aminomethylphosphonic acid and glufosinate in drinking water, different procedures of enrichment and cleanup were examined using anion exchange or SPE. In many cases interactions of, e.g. alkaline earth metal ions especially calcium could be observed during enrichment and cleanup resulting in loss of analytes. For that reason, a novel cleanup and enrichment procedure for the determination of these phosphonic acid herbicides has been developed in drinking water using cation‐exchange resin. In summary, the cleanup procedure with cation‐exchange resin developed in this study avoids interactions as described above and is applicable to calcium‐rich drinking water samples. After derivatization with 9‐fluorenylmethylchloroformate followed by LC with fluorescence detection, LOD of 12, 14 and 12 ng/L and mean recoveries from real‐world drinking water samples of 98±9, 100±16 and 101±11% were obtained for glyphosate, aminomethylphosphonic acid and glufosinate, respectively. The low LODs and the high precision permit the analysis of these phosphonic acid herbicides according to the guidelines of the European Commission.     

Cubic interaction vertices for fermionic and bosonic arbitrary spin fields

Using the light-cone gauge approach to relativistic field dynamics, we study arbitrary spin fermionic and bosonic fields propagating in flat space of dimension greater than or equal to four. Generating functions of parity invariant cubic interaction vertices for totally symmetric and mixed-symmetry massive and massless fields are obtained. For the case of totally symmetric fields, we derive restrictions on the allowed values of spins and the number of derivatives. These restrictions provide a complete classification of parity invariant cubic interaction vertices for totally symmetric fermionic and bosonic fields. As an example of application of the light-cone formalism, we obtain simple expressions for the Yang–Mills and gravitational interactions of massive arbitrary spin fermionic fields. For some particular cases, using our light-cone cubic vertices, we discuss the corresponding manifestly Lorentz invariant and on-shell gauge invariant cubic vertices.     

异烟肼与超氧化物歧化酶相互作用的光谱

利用紫外吸收光谱法和荧光光谱法研究了异烟肼(NH)与铜锌超氧化物歧化酶(Cu-ZnSOD)的结合作用。结果表明,NH与Cu-ZnSOD的结合行为使Cu-ZnSOD的内源荧光猝灭。通过猝灭常数、结合常数和结合位点的计算,证明荧光猝灭机理符合静态机制;NH和Cu-ZnSOD形成了1∶1稳定复合物,且复合物的形成不影响酶的活力。NH本身具有一定的超氧负离子清除能力。考察不同温度下的猝灭作用,进一步证实结合作用主要受范德华力和氢键驱动。     

Stress concentration/intensity around elliptical/circular cylinder shaped surface flaws in cross-ply plates and validity of St. Venant’s principle in the presence of interacting singularities

Effects of localized elliptical (circular being a special case) cylindrical surface flaws in laminated composite plates are investigated by using C°-type triangular composite plate elements, formulated on the assumptions of transverse inextensibility and layer-wise constant shear-angle theory (LCST). Numerical results for a cross-ply laminate compromised by the presence of an external part-through elliptical/circular cylindrical slot indicate the existence of severe cross-sectional warping in the vicinity of the surface flaw and plate boundaries. Furthermore, three-dimensional nature of the stress concentration factor in the neighborhood of the elliptical or circular cylinder shaped surface flaw boundary is clearly exhibited. Besides, very high stress concentration factors are found in the layer weakened by the surface flaw. Most importantly, the effects of stress singularity in the neighborhood of the circumferential re-entrant corner lines of the elliptical/circular cylindrical surface flaws, weakening laminated composite plates, are numerically assessed, because of their role in crack initiation. Finally, the interaction of this singularity with free edge stress singularity at the plate boundary, and the implication of such interactions (i.e., violation of St. Venant’s principle) in regards to testing of laminated composite specimens are thoroughly investigated.     

An analytic network process approach to operationalization of five forces model

The five forces model has been one of the most influential frameworks for strategic management. In contrast to its importance as a centerpiece of textbooks, however, it has attracted less attention from both academic researchers and practicing managers. This is due to its innate weakness, difficulty in operationalization. The vital requisites for operationalizing the five forces model are to deal with it as a complex system composed of interrelated forces and their sub-forces, and to prioritize them with consideration of their interdependency. The tenet of this study is the requisites can be achieved through the analytic network process (ANP). The ANP, which is a generalization of the analytic hierarchy process (AHP), produces priorities of elements in a complex network model with consideration of interdependency among elements. The five forces model is transformed into a network model of the ANP. The ANP procedure is then carried out to obtain the priority weights of the forces. Combining the derived weights and ratings on the forces produces the state-of-industry-competition index (SICI) values that represent the overall competitive condition of a given industry. The working of the proposed approach is provided with the help of a case study example of the Web portal Industry of Korea. The proposed ANP approach is expected to expand the five forces model into a workable system of analysis by improving its analytical power.     

Amplitude death: The emergence of stationarity in coupled nonlinear systems

When nonlinear dynamical systems are coupled, depending on the intrinsic dynamics and the manner in which the coupling is organized, a host of novel phenomena can arise. In this context, an important emergent phenomenon is the complete suppression of oscillations, formally termed amplitude death (AD). Oscillations of the entire system cease as a consequence of the interaction, leading to stationary behavior. The fixed points which the coupling stabilizes can be the otherwise unstable fixed points of the uncoupled system or can correspond to novel stationary points. Such behavior is of relevance in areas ranging from laser physics to the dynamics of biological systems. In this review we discuss the characteristics of the different coupling strategies and scenarios that lead to AD in a variety of different situations, and draw attention to several open issues and challenging problems for further study.