全文获取类型
收费全文 | 91篇 |
免费 | 2篇 |
国内免费 | 13篇 |
专业分类
化学 | 89篇 |
综合类 | 1篇 |
数学 | 10篇 |
物理学 | 6篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 3篇 |
2012年 | 13篇 |
2011年 | 7篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 3篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 3篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 7篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有106条查询结果,搜索用时 15 毫秒
81.
Chitosan Based Diamond Paste Stochastic Microsensors Modified with Gold Nanoparticles Detect Hepatitis C Virus Core Antigen
下载免费PDF全文
![点击此处可从《Electroanalysis》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Iuliana Moldoveanu Raluca‐Ioana Stefan‐van Staden Jacobus Frederick van Staden 《Electroanalysis》2015,27(8):1842-1846
Three types of chitosan (chitosan I (n=371–744), chitosan II (n=682–930), and chitosan III (n=868–1365)) as well as gold nanoparticles (10 nm diameter) were used to modify diamond paste for the design of new stochastic microsensors. Hepatitis C virus core antigen was used as model analyte to prove the stochastic behavior of the proposed microsensors. The microsensors cover a linear range of concentration between 40 fg/mL and 4 ng/mL. The highest sensitivity (1.38×105 s?1/mg/mL) and the lower limit of determination (40 fg/mL) were obtained for chitosan III based microsensor. The hepatitis C virus core antigen was assayed from whole blood samples with recoveries higher than 98.00 %. 相似文献
82.
可生物降解聚合物乙肝疫苗微球的制备和表征 总被引:5,自引:0,他引:5
采用双乳液体系(W1/O/W2)溶剂抽提法制备了聚-DL-乳酸-聚乙二醇嵌段共聚物(Poly-dllactide-b-polyethylene glycol,PELA)包裹乙肝表面抗原(HBsAg)微球。微球外观规整,平均粒径2.17μm,抗原包裹量达1.25%,包裹效率71.8%。经聚丙烯酰胺凝胶电泳(PAGE)检测,HBsAg在微球制备过程中保持了结构完整性。以乙肝常规铝佐剂疫苗为对照,BAL 相似文献
83.
锗元素和肝炎关系的研究 总被引:1,自引:0,他引:1
为研究肝炎和锗元素的关系,检测了肝炎患者在服用Ge--132治疗前和一或二个疗程治疗的头发、因清中锗元素含量,并设立对照组50例。治疗结果显示,患者临床症状和实验室检查有明显改善,时其昨有一定的临床和治疗价值。 相似文献
84.
L Jian-nan WANG Song XU Yan ZHANG Man-hua SIQING Tunala JI Shang-wei JIAO Jian WANG Xu WANG Jiang-bin 《高等学校化学研究》2012,28(1):79-83
The candidate polymorphism rs12979860 of interleukin 28B(IL28B) gene was genotyped by means of polymerase chain reaction(PCR) amplification and direct PCR sequencing, and then the role of this single nucleotide polymorphism(SNP) in the response to the treatment of 172 Chinese patients with hepatitis C virus(HCV) chronic infection was analyzed. The results show that the frequencies of major homozygotes(CC) and heterozygotes(CT) of rs12979860 were 0.84 and 0.16, respectively, and the minor homozygotes(TT) wasn't found in this cohort. A highly significant association was found between the CC genotype and sustained virological response(SVR) in HCV genotype 1 infected patients(P<0.001) but not in HCV non-genotype 1 infected patients. In addition, a strong association was found between rs12979860 CC genotype and rapid virological response(RVR) in both HCV genotype 1 infected patients(P<0.001) and non-genotype 1 infected patients(P<0.001). Taken together, these results indicate that IL28B polymorphism plays a key role in response to hepatitis C therapy in Chinese patients. Combine assessment of clinical predictors and the IL28B polymorphism may be beneficial to the individualized treatment decision in Asian chronic hepatitis C(CHC) patients. 相似文献
85.
P. Sospedra M. Espina M. Castro S. Corrales I. Haro C. Mestres 《Colloid and polymer science》2001,279(4):331-339
Mixed monolayers of an acylated derivative of hepatitis A synthetic peptide VP3(110-121) with neutral, cationic or anionic
lipids were spread at the air/water interface. Deviations from ideality as well as thermodynamic values were calculated at
different surface pressures using the free-excess energy, the interaction parameter and the enthalpy. The miscibility at the
collapse point was also checked. Maximum deviations from ideality were found for mixtures containing the anionic lipid phosphatidylglycerol
(PG), and it seems that the monolayer composition is not stable through compression, as the peptide is ejected from the film.
Films containing neutral [dipalmitoylphosphatidylcholine (DPPC)] or cationic [stearylamine (SA)] lipids showed more regular
behaviour. As the peptide has a net negative charge it is probable that electrostatic interactions are in part responsible
of the good miscibility of palmitoyl VP3(110-121) with SA. In order to prepare liposomes containing palmitoyl VP3(110–121),
lipids such as SA or DPPC/SA will be a more suitable choice than anionic lipids such as PG.
Received: 26 May 2000 Accepted: 22 September 2000 相似文献
86.
87.
建立运用表面加强激光解吸电离-飞行时间质谱获取乙肝病人和健康人血清蛋白指纹图谱数据,并用偏最小二乘(PLS)变量筛选法建立乙型肝炎(HBV)病人和健康人的分类模型,最终得到分类模型的交叉检验相关系数达0.97以上,判别准确率显著提高.对模型进行分析,找出对乙型肝炎病人和健康人的差异有重要影响的因素或变量.这些变量为某些质荷比区间内特定蛋白的峰强度值,反映这些质荷比区间内蛋白量的增加或减少,与乙肝病的形成有密切关系,可作为重要的生物标志物,进一步加以研究.本研究采用所得模型的拟合值等一些信息来做分类图,能较好地表达回归模型的分类效果. 相似文献
88.
Seung Up Kim Young Chul Kim Ji Soo Choi Kyung Sik Kim Gi Hong Choi Jin Sub Choi Jun Yong Park Do Young Kim Sang Hoon Ahn Eun Hee Choi Young Nyun Park Chae Yoon Chon Kwang-Hyub Han Myeong-Jin Kim 《Magnetic resonance imaging》2010
Liver fibrosis determines the functional liver reserve. Several studies have reported that the apparent diffusion coefficient (ADC) values of diffusion-weighted magnetic resonance imaging (DW-MRI) can assess liver fibrosis. We investigated whether DW-MRI predicts postoperative hepatic insufficiency and liver fibrosis in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Twenty-six patients with HBV-related HCC who received preoperative DW-MRI on a 3-T MRI system were enrolled between July and December 2008. ADC values were measured twice by two observers. Three “b values” were used: 50, 400 and 800 s/mm2. Postoperative hepatic insufficiency was defined as persistent hyperbilirubinemia (total bilirubin level >5 mg/dl for more than 5 days after surgery) or postoperative death without other causes. The mean age (21 men and 5 women) was 51.4 years. Three patients experienced postoperative hepatic insufficiency. liver stiffness measurement predicted postoperative hepatic insufficiency, advanced fibrosis (F3–4), and cirrhosis significantly [area under the receiving operator characteristic curve (AUROC)=0.942, 0.771 and 0.818, respectively, with P=.047, 0.048 and 0.006, respectively]; ADC values of DW-MRI, however, did not (AUROC=0.797, 0.648 and 0.491, respectively, with P=.100, 0.313 and 0.938, respectively). Reliability of ADC values between right and left hepatic lobes (ρ=0.868 and ρ=0.910 in the first and second measures of Observer A; ρ=0.865 and ρ=0.831 in the first and second measures of Observer B) was high and the intra- and interobserver reliability (ρ=0.958 in observer A and ρ=0.977 in observer B; ρ=0.929 in the first measure and ρ=0.978 in the second measure between the two observers) were high. All reliability was significant (P<.001). Our results suggest that DW-MRI on a 3-T MRI system is not suitable for predicting postoperative hepatic insufficiency, advanced liver fibrosis, and cirrhosis in patients with HBV-related HCC, despite significantly high reliability. 相似文献
89.
Though the prevalence of hepatitis B began to decline for the first time in 2010, it remains unclear whether this downward trend is permanent and the disease will be eradicated in mainland China under the current measures. Because a large number of hepatitis B virus (HBV) carriers and unknown HBV infections is characteristic of HBV infections in China, a mathematical model was designed and fitted to the reported hepatitis B data. The estimated basic reproduction number is 1.2861 (95\% confidence interval (CI) 1.2386-1.3302), which remains greater than one. Thus, the decline in 2010 may be part of the temporary benefits of public policy measures and should not be interpreted as indicative of successful intervention, although interventions do provide some benefits. To assess the effects of various interventions, the global uncertainty and sensitivity analyses revealed that the contribution of carriers is always greater than that of acute infections, and the prevalence of hepatitis B in China may be primarily a result of transmission by unknown patients. Therefore, strategies for controlling the HBV endemic, which target known patients, are unlikely to be highly effective. Additionally, three feasible strategies are proposed, although the benefits of these strategies may change radically over time. 相似文献
90.
Hepatitis B virus (HBV) is a major causative agent of human hepatitis. Its viral genome comprises partially double-stranded DNA, which is complexed with viral polymerase within an icosahedral capsid consisting of a dimeric core protein. Here, we describe the effects of capsid assembly modulators (CAMs) on the geometric or kinetic disruption of capsid construction and the virus life cycle. We highlight classical, early-generation CAMs such as heteroaryldihydropyrimidines, phenylpropenamides or sulfamoylbenzamides, and focus on the chemical structure and antiviral efficacy of recently identified non-classical CAMs, which consist of carboxamides, aryl ureas, bithiazoles, hydrazones, benzylpyridazinones, pyrimidines, quinolines, dyes, and antimicrobial compounds. We summarize the therapeutic efficacy of four representative classical compounds with data from clinical phase 1 studies in chronic HBV patients. Most of these compounds are in phase 2 trials, either as monotherapy or in combination with approved nucleos(t)ides drugs or other immunostimulatory molecules. As followers of the early CAMs, the therapeutic efficacy of several non-classical CAMs has been evaluated in humanized mouse models of HBV infection. It is expected that these next-generation HBV CAMs will be promising candidates for a series of extended human clinical trials. 相似文献