全文获取类型
收费全文 | 91篇 |
免费 | 2篇 |
国内免费 | 13篇 |
专业分类
化学 | 89篇 |
综合类 | 1篇 |
数学 | 10篇 |
物理学 | 6篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 3篇 |
2012年 | 13篇 |
2011年 | 7篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 3篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2002年 | 1篇 |
2001年 | 3篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 7篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有106条查询结果,搜索用时 15 毫秒
41.
《Analytical letters》2012,45(4):592-605
This work reports an efficient method to quantify the Hepatitis B surface antigen and α-fetoprotein in human serum using a functional magnetic nanoparticle-assisted sandwich-type electrochemical immunoassay. The Fe 3 O 4 magnetic nanoparticles were first modified with carboxyl functional groups to permit stable bioconjugation to the amine groups of most biological targets. The primary antibodies were then covalently stained on the surface of the functional magnetic nanoparticles, followed by the analyte and secondary antibodies, resulting in a sandwich-type (antibody-antigen-antibody/enzyme) immune complex. The secondary antibodies were labeled with horseradish peroxidase for the catalytic oxidation of 2-aminophenol to yield electrochemically reducible molecules. The separation using an external magnetic field guaranteed fast and reliable purification and enrichment of analytes. Quantitative analysis was performed upon representative clinical targets: Hepatitis B surface antigen and α-fetoprotein in human serum. The detection limits were 0.06 ng/mL for the former and 0.5 ng/mL for the latter, which were about 10 times lower than values obtained by conventional enzyme-linked immunosorbent assays. The reported method may be adopted as a general strategy for the sensitive and selective determination of additional proteins and biological molecules. 相似文献
42.
A mathematical model is formulated to describe the spread of hepatitis B. The stability of equilibria and persistence of disease are analyzed. The results shows that the dynamics of the model is completely determined by the basic reproductive number ρ0. If ρ0 < 1, the disease-free equilibrium is globally stable. When ρ0 > 1, the disease-free equilibrium is unstable and the disease is uniformly persistent. Furthermore, under certain conditions, it is proved that the endemic equilibrium is globally attractive. Numerical simulations are conducted to demonstrate our theoretical results. The model is applied to HBV transmission in China. The parameter values of the model are estimated based on available HBV epidemic data in China. The simulation results matches the HBV epidemic data in China approximately. 相似文献
43.
通过捕获探针与纳米金膜之间的共价连接, 保证了滚环扩增(RCA)产物始终结合于金膜表面, Phi29 DNA聚合酶的高效扩增和Escherichia coli DNA链接酶的高度精确性使检测达到单碱基识别, 检测灵敏度达到104 copies/mL. 实验结果表明, 与单碱基错配序列相比, RCA可明显增强检测的灵敏度. 该RCA基因传感器操作简单, 灵敏度和特异性较高, 在乙型肝炎病毒的快速检测方面具有一定的开发潜力. 相似文献
44.
用于乙肝表面抗原检测的压电免疫传感器的研制 总被引:6,自引:0,他引:6
研制了一种用于乙肝表面抗原(HBsAg)检测的新型传感器———石英压电免疫传感器。采用聚乙烯亚胺粘附和戊二醛交联法在金电极上固定抗体蛋白,对固定化过程进行了监测。HBsAg含量的检测范围为1~40mg/L。使用过的电极用02mol/L乙醇胺(pH=8)解吸,可重复使用。将此传感器用于实际血清样品的检测,与酶联免疫吸附法所得结果吻合。 相似文献
45.
乙肝病毒聚合酶链反应扩增产物的毛细管电泳测定 总被引:3,自引:2,他引:3
本文用毛细管电泳对乙肝病毒聚合酶反应扩增产物进行了检测,并用这一方法和酶联免疫吸附实验以及PCR与传统电泳联用的方法进行比较,探讨了用高效,快速,微量,易自动化的毛细管电泳代替传统检测方法的可能性,取得了令人满意的结果。 相似文献
46.
The cholesterol molecules interact with p7 channel between the adjoint H3 helixes, resulting in prominent conformational changes of these helical segments, which further exerts influence on the His17 residues of i + 2 monomer, leading to the residues facing towards to the lumen of the pore. Such side chain orientations of His17 are considered to be essential for the channel’s ionic selectivity and gating. 相似文献
47.
将Zr-8-羟基喹啉-5-磺酸荧光体系与对氟苯酚为底物的酶促反应相偶合,建立了一种测定辣根过氧化物酶(HRP)及其标记物的新方法.测定HRP的线性范围为0.031~31mU/mL,检出限(3σ)为0.007mU/mL.用于测定人血清中乙肝表面抗原和E抗体,结果令人满意. 相似文献
48.
DNA detection plays an important role in early diagnosis of genetic disease. The conventional detection methods of DNA are based on expensive equipment, which do not meet the demands of developing countries. Thus, we developed a colorimetric method, which could be observed with naked eye and used copper nanoclusters for cost-effective. Moreover, the target of this method is the DNA in Hepatitis B virus that is one of the most popular chronic viral infections in developing countries over the past years. Our method was sensitive and the limit of detection was 12 × 109 molecules. Three-base-pair mismatches target DNA was detected easily. These results revealed the favorable sensitivity and selectivity of this approach. Most importantly, our method may have potential applications in correct diagnosis of genetic disease and monitoring of gene therapy in the poverty-stricken areas. 相似文献
49.
Sheng Tang Zong-Gen Peng Xin Zhang Xin-Yue Cheng Wen-Jing Li Jian-Dong Jiang Ying-Hong Li Dan-Qing Song 《中国化学快报》2016,27(7):1052-1057
A new series of 12-benzyl matrinic amide/ethanamide derivatives were synthesized from matrinine(1)and evaluated for their anti-HCV activity,taking compound 2 as the lead.SAR revealed that the introduction of a suitable substituent at the N'-end of matrinic amide might greatly enhance the potency.Among them,matrinic acid 17 and N'-substituted matrinic amides 18a-d exhibited promising potency with low micromolar EC_(50) values ranging from 1.03μmol/L to 7.54 μmol/L,and better therapeutic window with SI from 66 to 132.Moreover,compound 17 displayed an excellent PK and safety profile in vivo,demonstrating good drug-like characteristics.Thus,it has been selected for further investigation,with an advantage of decreased chances of inducing drug-resistance mutations. 相似文献
50.
N‐Arylaminomethyl‐3H‐1,3,4‐oxadiazole‐2‐thiones 2a,b were prepared from the corresponding N‐arylglycinoylhydrazides. A number of their thioglycoside derivatives 4–7a–c and S‐functionalized analogs 8–11a,b were synthesized by the reaction with different acetobromosugars and acyclic hydroxyalkylating agents. The antiviral activity of a number of the synthesized compounds against herpes simplex virus type 1 (HSV‐1) and hepatitis A virus (HAV) was evaluated. Compounds 5a and 5b showed promising results against HAV. 相似文献