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排序方式: 共有251条查询结果,搜索用时 15 毫秒
91.
Emiko Kinoshita‐Kikuta Hiroshi Kusamoto Syogo Ono Keisuke Akayama Yoko Eguchi Masayuki Igarashi Toshihide Okajima Ryutaro Utsumi Eiji Kinoshita Tohru Koike 《Electrophoresis》2019,40(22):3005-3013
In the bacterial signaling mechanisms known as two‐component systems (TCSs), signals are generally conveyed by means of a His–Asp phosphorelay. Each system consists of a histidine kinase (HK) and its cognate response regulator. Because of the labile nature of phosphorylated His and Asp residues, few approaches are available that permit a quantitative analysis of their phosphorylation status. Here, we show that the Phos‐tag dye technology is suitable for the fluorescent detection of His‐ and Asp‐phosphorylated proteins separated by SDS‐PAGE. The dynamics of the His–Asp phosphorelay of recombinant EnvZ‐OmpR, a TCS derived from Escherichia coli, were examined by SDS‐PAGE followed by simple rapid staining with Phos‐tag Magenta fluorescent dye. The technique permitted not only the quantitative monitoring of the autophosphorylation reactions of EnvZ and OmpR in the presence of adenosine triphosphate (ATP) or acetyl phosphate, respectively, but also that of the phosphotransfer reaction from EnvZ to OmpR, which occurs within 1 min in the presence of ATP. Furthermore, we demonstrate profiling of waldiomycin, an HK inhibitor, by using the Phos‐tag Cyan gel staining. We believe that the Phos‐tag dye technology provides a simple and convenient fluorometric approach for screening of HK inhibitors that have potential as new antimicrobial agents. 相似文献
92.
Chun Cai Wen-Bin Yi Wei Zhang Ming-Gui Shen Mei Hong Li-Yan Zeng 《Molecular diversity》2009,13(2):209-239
The new phase-separation and immobilization technique known as fluorous biphase system (FBS) has become an active topic among
researchers in both industry and academia. A series of fluorous biphasic reactions catalyzed by Lewis acid-type metal perfluorooctanesulfonates
and metal bis(perfluorooctanesulfonyl)amides are reviewed in this paper. The recent development of fluorous phase transfer
catalyst (PTC) is also discussed. 相似文献
93.
基于原子蒸汽池中铯里德伯原子的电磁感应透明光谱在微波场作用下的Aulter-Towns效应,测量了无芯射频识别标签线形散射单元的近场散射微波电场二维空间分布,空间分辨率可达到亚微波波长.实现了射频电场极化方向与线形散射体标签夹角的有效分辨.电磁仿真软件的仿真结果与实验测量符合得很好.本研究提供了一种测量微波电场近场测量的新方法,对无芯射频识别标签的散射单元设计和标定以及电子电路的电磁辐射测量具有重要的意义. 相似文献
94.
在表面活性剂油酸和油胺,液相环境二苄醚体系中,利用多元醇还原法,采用1,2-十二烷二醇还原前驱体乙酰丙酮铁Fe(acac)3,通过表面活性剂、金属前驱体以及液相环境的共同作用,制备出了单分散片状六边形Fe3O4纳米颗粒。分析了表面活性剂以及还原剂多元醇对纳米颗粒尺寸及形貌的影响。TEM表征结果显示:与未使用表面活性剂的情况相比,油酸和油胺的加入抑制了颗粒的生长,使颗粒尺寸从24.2 nm降低到10.7 nm;颗粒形貌多样化,出现了片状六边形形貌的Fe3O4纳米颗粒。磁性能检测表明: Fe3O4纳米颗粒具有高饱和磁化强度(Ms=88 emu/g)和零剩磁的特点,有望作为磁标记材料应用在生物检测上 相似文献
95.
A new synthetic protocol for sclerotigenin-type benzodiazepine-quinazolinone library scaffold is introduced. A fluorous benzyl protecting group is used for the synthesis of 4-benzodiazepine-2,5-dione intermediate and also as a phase tag for fluorous solid-phase extraction (F-SPE). 相似文献
96.
Wei Zhang Tadamichi Nagashima Yimin Lu Christine Hiu-Tung Chen 《Tetrahedron letters》2004,45(24):4611-4613
The perfluorooctylsulfonyl group is introduced as a traceless tag for solution-phase palladium-catalyzed deoxygenation reactions. The synthetic efficiency is improved by microwave irradiation for reaction and fluorous solid-phase extraction for separation. Further application of this traceless tag for multistep synthesis of substituted hydantoin and pyrimidine is also described. 相似文献
97.
The novel fluorous support Hfb (hexakisfluorous chain-type butanoyl) was easily prepared. The Hfb group was readily introduced into the anomeric hydroxyl group of a carbohydrate, and was recyclable after cleavage. The use of the Hfb group was applicable for the rapid oligosaccharide synthesis in which the synthetic intermediates could be purified using fluorous and normal organic solvents. Each synthetic intermediate could be monitored by TLC, NMR and mass spectrometry. 相似文献
98.
Lucie ?ervenková Št’astná Jaroslav Kví?ala 《Journal of organometallic chemistry》2007,692(10):1974-1982
Fluorous partition coefficients in perfluorohexane/toluene system for 37 fluorinated silylcyclopentadienes, titanium(IV) complexes derived from them, perfluoroalkyl substituted tetramethylcyclopentadienes, and their Rh(III) and Rh(I) complexes were determined. Specific fluorophilicity and fluorousness according to Kiss and Rábai were calculated for each compound with the help of molecular volume computed with the Gaussian program. The results show relative unimportance of fluorine content parameter for fluorophilicity as shown by a rhodium(I) complex being fluorophilic at fluorine content as low as 46.3%. As expected, fluorophilicity increased with the fluorous ponytail length and ponytail number in series of similar compounds, whereas polar M-Cl bonds were decreasing it. Fluorophilicities of tetramethyl(perfluoroalkyl)cyclopentadiene tautomers varied considerably despite only small differences in molecular volume being found. Most of the compounds were prepared previously, several new silylcyclopentadienes and titanium(IV) silylcyclopentadienyl complexes are reported here for completion. 相似文献
99.
The roles of the element fluorine and its compounds in relationship to green chemistry and clean chemical manufacturing are considered. 相似文献
100.
Dithiophosphoric acids [HS2P(OC2H4CnF2n+1)2] (n = 4, 6) have been prepared in high yields. Deprotonation and reaction with transition metal substrates affords fluorous metal complexes which have been characterised by elemental analysis, mass spectrometry, IR and NMR spectroscopies. The structures of [Cu{S2P(OC2H4CnF2n+1)2}(PPh3)2] (n = 4, 6) and [Cu{-S2P(OC2H4C4F9)2}(PPh3)]2 have been determined by single crystal X-ray diffraction. 相似文献